Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   VV7356_RS20230 Genome accession   NZ_CP041683
Coordinates   4520960..4522648 (+) Length   562 a.a.
NCBI ID   WP_017789876.1    Uniprot ID   -
Organism   Vibrio vulnificus strain 7356     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4515960..4527648
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VV7356_RS20210 (VV7356_20790) pdhR 4517562..4518329 (-) 768 WP_011079554.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  VV7356_RS20215 (VV7356_20795) ampD 4518718..4519290 (-) 573 WP_017789879.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  VV7356_RS20220 (VV7356_20800) nadC 4519383..4520270 (+) 888 WP_017789878.1 carboxylating nicotinate-nucleotide diphosphorylase -
  VV7356_RS20225 (VV7356_20805) pilA 4520493..4520960 (+) 468 WP_017789877.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  VV7356_RS20230 (VV7356_20810) pilB 4520960..4522648 (+) 1689 WP_017789876.1 type IV-A pilus assembly ATPase PilB Machinery gene
  VV7356_RS20235 (VV7356_20815) pilC 4522696..4523922 (+) 1227 WP_017789875.1 type II secretion system F family protein Machinery gene
  VV7356_RS20240 (VV7356_20820) pilD 4524016..4524885 (+) 870 WP_026130630.1 prepilin peptidase Machinery gene
  VV7356_RS20245 (VV7356_20825) coaE 4524887..4525495 (+) 609 WP_017789873.1 dephospho-CoA kinase -
  VV7356_RS20250 (VV7356_20830) zapD 4525528..4526268 (+) 741 WP_017789872.1 cell division protein ZapD -
  VV7356_RS20255 (VV7356_20835) yacG 4526331..4526525 (+) 195 WP_026060801.1 DNA gyrase inhibitor YacG -
  VV7356_RS20260 (VV7356_20840) rplS 4526742..4527095 (-) 354 WP_011079544.1 50S ribosomal protein L19 -

Sequence

Protein


Download         Length: 562 a.a.        Molecular weight: 62370.37 Da        Isoelectric Point: 5.5349

>NTDB_id=373020 VV7356_RS20230 WP_017789876.1 4520960..4522648(+) (pilB) [Vibrio vulnificus strain 7356]
MQSNLATILRQANQLSLTQEQACRETIQASGVTAPEALLQLGFFQPDELTEQLSAIFGLPRTDLNQYDYQELCNQLGLRE
LITRHQALPLQKSNAGLILAVADPTNIQAEDDFRFATGVQVEIVLADCQELQAAIRRLYGKSIKQSQGSGLKEIHPDELA
NLVEVSDDEVESIEDLSQDESPVSRFINQILLDAVRKGASDIHFEPYENQYRVRLRCDGILVEVQQPPSHLSRRLSARIK
ILSKLDIAERRLPQDGRIKLRLNQETAIDMRVSTLPTLFGEKIVLRLLDSSSSSLDIDKLGYNPQQKQLYLDALRKPQGM
ILMTGPTGSGKTVSLYTGLSLLNRPEVNISTAEDPVEINLPGINQVQVKPKIGFGFAEALRSFLRQDPDIVMVGEIRDLE
TAEIAVKAAQTGHLVLSTLHTNSAAETIVRLSNMGIASFNLASSLSLIIAQRLARRLCSHCKQPHPYNATFELLGLKADD
LIYQADKQGCHHCTHGYSGRVGIYEVMPFDEQLAEAIVKGASVQSLEMLARQKGMMTLKDSGLEKLKQGITSLEELQRVL
YL

Nucleotide


Download         Length: 1689 bp        

>NTDB_id=373020 VV7356_RS20230 WP_017789876.1 4520960..4522648(+) (pilB) [Vibrio vulnificus strain 7356]
ATGCAATCCAACCTTGCCACGATTCTTCGTCAGGCCAACCAACTTAGCCTAACGCAAGAACAAGCGTGTCGAGAGACAAT
ACAAGCTTCTGGTGTGACAGCACCGGAAGCTTTGTTGCAACTGGGATTTTTCCAGCCGGATGAATTAACCGAGCAGCTAA
GCGCGATTTTTGGCTTACCACGCACTGACCTCAACCAATATGACTACCAAGAGCTGTGTAATCAACTTGGGTTGCGTGAG
CTGATCACCCGTCACCAAGCATTGCCACTGCAAAAAAGCAACGCTGGTTTAATACTCGCGGTTGCTGACCCTACCAATAT
TCAAGCTGAAGATGATTTCCGTTTTGCCACAGGTGTGCAGGTGGAGATTGTTTTGGCCGATTGCCAAGAGCTGCAAGCGG
CGATTCGTAGACTCTATGGAAAGAGCATCAAACAGAGCCAAGGCTCTGGTTTAAAAGAGATTCACCCAGATGAACTGGCG
AATTTAGTCGAGGTTTCAGATGACGAAGTCGAGTCCATTGAAGACTTAAGCCAAGATGAATCTCCCGTTAGCCGCTTTAT
CAACCAAATTCTGCTGGATGCGGTACGCAAAGGCGCATCTGATATTCATTTTGAACCGTACGAAAACCAATACCGCGTGC
GATTGCGTTGCGATGGTATTTTGGTGGAAGTGCAGCAGCCCCCAAGCCATTTAAGCCGCCGTTTATCCGCGAGGATCAAG
ATCCTCTCCAAGTTGGATATTGCGGAAAGGCGTCTGCCACAAGACGGACGTATTAAGCTACGCCTCAACCAAGAAACCGC
GATTGATATGCGCGTTTCCACCTTACCCACACTGTTTGGTGAAAAAATTGTGTTGCGACTTCTCGACAGCTCGAGCAGTA
GCTTGGATATTGATAAGTTAGGCTACAACCCGCAACAAAAACAGCTCTACCTCGATGCCCTGCGCAAACCGCAAGGGATG
ATTTTGATGACGGGCCCAACAGGAAGCGGCAAAACCGTTTCGCTGTACACAGGCTTGAGTTTACTTAACCGGCCAGAAGT
GAATATCTCCACCGCGGAAGACCCAGTCGAGATTAACCTCCCGGGAATCAACCAAGTGCAAGTGAAACCCAAAATTGGTT
TTGGTTTTGCCGAAGCATTGCGTTCTTTTCTCCGTCAAGACCCGGATATCGTCATGGTGGGGGAAATTCGAGATTTGGAA
ACCGCCGAGATCGCCGTCAAAGCAGCCCAAACCGGTCACCTTGTGCTATCCACCTTACATACCAACTCGGCAGCTGAAAC
CATTGTTCGTCTATCTAACATGGGGATCGCCAGCTTTAACTTAGCTTCATCCTTGAGTTTGATCATTGCTCAGCGTCTGG
CTCGTCGGTTATGCAGCCATTGCAAGCAACCTCATCCATACAACGCAACATTTGAGTTACTCGGTCTAAAAGCTGACGAC
TTGATTTATCAAGCTGATAAACAGGGTTGCCATCATTGTACTCATGGTTATTCCGGTCGTGTCGGTATATACGAAGTCAT
GCCTTTTGATGAGCAGCTTGCAGAGGCGATCGTTAAAGGCGCCTCGGTACAAAGCTTGGAGATGCTTGCTCGGCAAAAAG
GCATGATGACACTCAAAGATTCCGGCCTTGAAAAACTCAAACAGGGCATCACCAGCTTAGAAGAGCTGCAACGCGTCCTC
TATTTGTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(38-141)

Type II/IV secretion system protein (T2SSE)

(189-456)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio campbellii strain DS40M4

76.335

100

0.763
  pilB Vibrio parahaemolyticus RIMD 2210633

75.801

100

0.758
  pilB Vibrio cholerae strain A1552

74.245

100

0.744
  pilB Legionella pneumophila strain ERS1305867

53.216

91.281

0.486
  pilB Acinetobacter baumannii D1279779

51.341

92.883

0.477
  pilB Acinetobacter baylyi ADP1

50.383

92.883

0.468
  pilF Neisseria gonorrhoeae MS11

45.31

100

0.456
  pilF Thermus thermophilus HB27

40.395

99.11

0.4
  pilB/pilB1 Synechocystis sp. PCC 6803

36.888

100

0.375
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.491

94.306

0.363

Multiple sequence alignment