Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   VV7356_RS20225 Genome accession   NZ_CP041683
Coordinates   4520493..4520960 (+) Length   155 a.a.
NCBI ID   WP_017789877.1    Uniprot ID   -
Organism   Vibrio vulnificus strain 7356     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4515493..4525960
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VV7356_RS20210 (VV7356_20790) pdhR 4517562..4518329 (-) 768 WP_011079554.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  VV7356_RS20215 (VV7356_20795) ampD 4518718..4519290 (-) 573 WP_017789879.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  VV7356_RS20220 (VV7356_20800) nadC 4519383..4520270 (+) 888 WP_017789878.1 carboxylating nicotinate-nucleotide diphosphorylase -
  VV7356_RS20225 (VV7356_20805) pilA 4520493..4520960 (+) 468 WP_017789877.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  VV7356_RS20230 (VV7356_20810) pilB 4520960..4522648 (+) 1689 WP_017789876.1 type IV-A pilus assembly ATPase PilB Machinery gene
  VV7356_RS20235 (VV7356_20815) pilC 4522696..4523922 (+) 1227 WP_017789875.1 type II secretion system F family protein Machinery gene
  VV7356_RS20240 (VV7356_20820) pilD 4524016..4524885 (+) 870 WP_026130630.1 prepilin peptidase Machinery gene
  VV7356_RS20245 (VV7356_20825) coaE 4524887..4525495 (+) 609 WP_017789873.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 155 a.a.        Molecular weight: 16299.82 Da        Isoelectric Point: 8.7897

>NTDB_id=373019 VV7356_RS20225 WP_017789877.1 4520493..4520960(+) (pilA) [Vibrio vulnificus strain 7356]
MKKLNKTKKQQGFTLIELMIVVAIIGILAAFAIPAYQDYTKRATMAEFPRVASSTKLAVELCAHEHAADGAGFKSKCYLA
ANGIPAEFTLNNLKVTPTTGTASGSVDITVAATADKGSIKTGEKYILTATYNATGITWEAVCKDAGGTAQTAYCP

Nucleotide


Download         Length: 468 bp        

>NTDB_id=373019 VV7356_RS20225 WP_017789877.1 4520493..4520960(+) (pilA) [Vibrio vulnificus strain 7356]
ATGAAGAAATTGAACAAAACCAAGAAACAACAAGGTTTTACCTTGATTGAATTAATGATAGTGGTGGCGATTATTGGTAT
TTTGGCCGCCTTTGCTATTCCTGCTTATCAAGACTACACAAAGAGAGCTACTATGGCCGAGTTTCCGAGAGTAGCGTCTT
CAACAAAATTAGCAGTCGAACTTTGCGCTCACGAACATGCAGCAGATGGAGCTGGATTCAAGTCAAAATGCTACCTAGCA
GCAAATGGTATTCCTGCAGAGTTTACGTTAAACAATTTAAAAGTAACTCCAACCACAGGAACTGCATCAGGTTCCGTTGA
CATTACAGTAGCAGCAACAGCAGACAAAGGCTCTATTAAGACCGGTGAAAAGTATATTCTTACCGCAACCTATAACGCTA
CTGGCATTACATGGGAAGCAGTTTGTAAAGATGCTGGTGGTACAGCACAAACAGCCTACTGTCCATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(8-32)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Vibrio campbellii strain DS40M4

74.51

98.71

0.735
  pilA2 Legionella pneumophila strain ERS1305867

43.919

95.484

0.419
  pilA2 Legionella pneumophila str. Paris

43.243

95.484

0.413
  pilA Haemophilus influenzae 86-028NP

40.278

92.903

0.374

Multiple sequence alignment