Detailed information NTG0371509

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: FLP15_RS11860
• Genome accession: NZ_CP041356
• Coordinates: 2503752..2504408 (-)
• Length: 218 a.a.
• NCBI ID: WP_142767279.1
• Uniprot ID: A0A514ZAX5
• Organism: Lactococcus protaetiae strain KACC 19320
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2498752..2509408

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
FLP15_RS11840 (FLP15_11840)	atpB	2499135..2499848 (-)	714	WP_120772196.1	F0F1 ATP synthase subunit A	-
FLP15_RS11845 (FLP15_11845)	-	2499894..2500100 (-)	207	WP_120772197.1	F0F1 ATP synthase subunit C	-
FLP15_RS11850 (FLP15_11850)	-	2500339..2501115 (-)	777	WP_142767277.1	alpha/beta hydrolase family protein	-
FLP15_RS11855 (FLP15_11855)	comEC	2501528..2503771 (-)	2244	WP_142767278.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
FLP15_RS11860 (FLP15_11860)	comEA	2503752..2504408 (-)	657	WP_142767279.1	helix-hairpin-helix domain-containing protein	Machinery gene
FLP15_RS11865 (FLP15_11865)	-	2504522..2505913 (-)	1392	WP_142767280.1	ABC transporter permease	-
FLP15_RS11870 (FLP15_11870)	-	2505910..2506866 (-)	957	WP_142767281.1	ABC transporter ATP-binding protein	-
FLP15_RS11875 (FLP15_11875)	-	2507537..2508064 (-)	528	WP_142767282.1	hypothetical protein	-
FLP15_RS11880 (FLP15_11880)	-	2508065..2508265 (-)	201	WP_142767283.1	helix-turn-helix transcriptional regulator	-
FLP15_RS11885 (FLP15_11885)	-	2508386..2508793 (-)	408	WP_142767284.1	hypothetical protein	-

Sequence

Protein

• Length: 218 a.a.
• Molecular weight: 23281.14 Da
• Isoelectric Point: 4.6229

>NTDB_id=371509 FLP15_RS11860 WP_142767279.1 2503752..2504408(-) (comEA) [Lactococcus protaetiae strain KACC 19320]
MERIIEFIKENLKLVILSVVALFAGGIFYFVSHPKSTDSTMSATALSSVSADKTSVSTSNHFSKATEIEVDLKGAVMKPN
VYDVSSDMRVDDLIKLAGGFTTNADQNQVNLAAKLKDEEVVYVPQKGEMASGVSADSSVSTDGVGNSLTTTSQTSKVNIN
TADLTQLQTLNGIGQKKAQDIIDYRTQNGNFQSVEDLGNVPGFGDKTIAKLKDSICVD

Nucleotide

• Length: 657 bp

>NTDB_id=371509 FLP15_RS11860 WP_142767279.1 2503752..2504408(-) (comEA) [Lactococcus protaetiae strain KACC 19320]
ATGGAAAGAATAATCGAATTTATCAAAGAAAATTTGAAACTTGTGATTCTATCAGTTGTTGCACTGTTTGCTGGTGGAAT
CTTTTATTTTGTCAGTCATCCGAAAAGTACTGACAGCACAATGTCAGCAACAGCATTGAGTTCTGTCAGTGCTGACAAAA
CTTCTGTCAGTACATCAAATCATTTCTCAAAAGCGACAGAAATCGAGGTTGACCTCAAAGGAGCAGTTATGAAACCAAAT
GTCTATGATGTTTCGAGCGATATGCGTGTTGATGATTTGATTAAATTGGCTGGAGGTTTTACTACAAATGCAGATCAAAA
TCAGGTTAATTTAGCAGCAAAATTAAAAGATGAAGAGGTGGTGTATGTTCCTCAAAAGGGAGAAATGGCAAGCGGAGTAT
CTGCTGACAGCTCTGTCAGTACTGACGGAGTGGGAAATTCACTGACAACCACATCACAAACATCAAAAGTAAATATCAAC
ACTGCCGATTTGACACAGCTTCAGACTTTGAACGGTATTGGTCAAAAGAAGGCGCAAGATATCATTGATTATCGTACTCA
AAATGGTAATTTTCAATCAGTAGAAGACCTCGGAAATGTACCAGGTTTTGGCGACAAAACAATTGCAAAATTGAAAGATT
CTATCTGTGTTGACTAA

Domains

Predicted by InterproScan.

• SLBB domain (SLBB) (70-123)

• Helix-hairpin-helix motif (HHH_3) (153-215)

3D structure

• AlphaFold DB: A0A514ZAX5

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Lactococcus lactis subsp. cremoris KW2	56.561	100	0.573
comEA/celA/cilE	Streptococcus mitis NCTC 12261	41.778	100	0.431
comEA/celA/cilE	Streptococcus pneumoniae D39	41.964	100	0.431
comEA/celA/cilE	Streptococcus pneumoniae R6	41.964	100	0.431
comEA/celA/cilE	Streptococcus pneumoniae Rx1	41.964	100	0.431
comEA/celA/cilE	Streptococcus mitis SK321	41.518	100	0.427
comEA	Streptococcus thermophilus LMD-9	39.316	100	0.422
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	41.071	100	0.422
comEA	Latilactobacillus sakei subsp. sakei 23K	38.288	100	0.39
comEA	Staphylococcus aureus N315	35.345	100	0.376
comEA	Staphylococcus aureus MW2	34.914	100	0.372