Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   FIU11_RS15560 Genome accession   NZ_CP040990
Coordinates   3229772..3231460 (+) Length   562 a.a.
NCBI ID   WP_004729083.1    Uniprot ID   -
Organism   Vibrio furnissii strain FDAARGOS_777     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3224772..3236460
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FIU11_RS15530 (FIU11_15530) dsbC 3225092..3225856 (-) 765 WP_004729072.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
  FIU11_RS15535 (FIU11_15535) xerD 3225873..3226727 (-) 855 WP_231623439.1 site-specific tyrosine recombinase XerD -
  FIU11_RS15540 (FIU11_15540) fldB 3226923..3227444 (+) 522 WP_004729075.1 flavodoxin FldB -
  FIU11_RS15545 (FIU11_15545) ampD 3227480..3228034 (-) 555 WP_004729077.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  FIU11_RS15550 (FIU11_15550) nadC 3228183..3229070 (+) 888 WP_004729079.1 carboxylating nicotinate-nucleotide diphosphorylase -
  FIU11_RS15555 (FIU11_15555) - 3229317..3229766 (+) 450 WP_004729081.1 pilin -
  FIU11_RS15560 (FIU11_15560) pilB 3229772..3231460 (+) 1689 WP_004729083.1 type IV-A pilus assembly ATPase PilB Machinery gene
  FIU11_RS15565 (FIU11_15565) pilC 3231539..3232765 (+) 1227 WP_004729085.1 type II secretion system F family protein Machinery gene
  FIU11_RS15570 (FIU11_15570) pilD 3232817..3233686 (+) 870 WP_004729087.1 prepilin peptidase Machinery gene
  FIU11_RS15575 (FIU11_15575) coaE 3233689..3234297 (+) 609 WP_004729089.1 dephospho-CoA kinase -
  FIU11_RS15580 (FIU11_15580) zapD 3234330..3235070 (+) 741 WP_004729090.1 cell division protein ZapD -
  FIU11_RS15585 (FIU11_15585) yacG 3235128..3235325 (+) 198 WP_004729092.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 562 a.a.        Molecular weight: 62058.19 Da        Isoelectric Point: 6.1994

>NTDB_id=368544 FIU11_RS15560 WP_004729083.1 3229772..3231460(+) (pilB) [Vibrio furnissii strain FDAARGOS_777]
MLTNLIAILRQAKLISPTQEQAVIDHIHASGATVPEALIDLAIFHHHDLTEQLSQIFGLPETDLTRFDYAPLCQQLGLRE
LITRYQALPLHSNGQILTLAVADPTHLQVEDEFRFATGLQVELVLADHKALHAAIRRLYGRSLATPGTQGKEISQDELAD
LVQISDDEKTTIEDLSQDDSPVSRFINQILLDAVRKGASDIHFEPYESHYRIRLRCDGILVETQQPASHLSRRLAARIKI
LSKLDIAERRLPQDGRIKLRLDADTAIDMRVSTLPTLWGEKIVLRLLDSSAANLNIDKLGYNEQQKQLYLAALTKPQGMI
LMTGPTGSGKTVSLYTGLRILNTEQVNISTAEDPVEINLPGINQVQVQPKIGFGFAQALRSFLRQDPDVVMVGEIRDLET
AEIAIKAAQTGHLVLSTLHTNSAAETVIRLANMGIEPFNLASSLSLIIAQRLARRLCPHCKVAITPPAALQQQFGLLAED
AVYEAHPSGCTECTNGYSGRVGIYEVMAFHPALADAVMQRASIHQIETIAQQHGMQPLLQSGIEKLRAGITSFSELQRVL
YF

Nucleotide


Download         Length: 1689 bp        

>NTDB_id=368544 FIU11_RS15560 WP_004729083.1 3229772..3231460(+) (pilB) [Vibrio furnissii strain FDAARGOS_777]
ATGCTGACCAACCTTATCGCCATTCTGCGTCAGGCTAAGTTGATCAGCCCAACGCAGGAACAGGCCGTGATCGATCATAT
TCACGCTTCCGGTGCCACGGTGCCGGAGGCGTTGATCGATTTGGCGATATTTCATCATCACGATTTAACCGAGCAACTGA
GTCAGATCTTTGGCCTACCCGAAACCGATCTCACTCGGTTTGATTACGCGCCATTGTGCCAGCAGTTGGGACTTCGAGAA
CTCATCACCCGCTACCAAGCCCTGCCGCTGCACAGCAATGGCCAAATCCTGACGTTGGCGGTTGCTGACCCAACTCACCT
GCAAGTCGAAGACGAGTTTCGCTTTGCCACCGGATTACAGGTGGAACTGGTGCTCGCCGATCACAAGGCGCTGCATGCCG
CCATTCGCCGCTTGTATGGGCGCAGCCTTGCGACGCCGGGGACGCAAGGCAAAGAGATCAGTCAGGATGAATTGGCGGAT
CTGGTACAGATCTCTGACGACGAAAAAACCACCATCGAAGATCTCAGCCAAGATGATTCACCGGTCAGTCGTTTTATCAA
CCAGATCCTACTCGATGCCGTGCGCAAAGGTGCGTCCGACATTCACTTTGAGCCGTATGAGTCCCACTACCGTATTCGTC
TACGCTGTGACGGCATTCTGGTCGAAACCCAACAACCAGCCAGTCACCTCAGCCGACGCTTGGCAGCCCGAATCAAAATT
TTGTCGAAATTAGATATTGCCGAGCGCCGCCTCCCGCAAGATGGACGCATCAAACTGCGCTTGGATGCCGACACCGCGAT
TGATATGCGTGTATCGACCTTGCCAACATTGTGGGGTGAAAAGATTGTTCTGCGCCTACTCGACAGCAGTGCGGCCAACC
TCAACATCGACAAACTCGGCTACAACGAGCAGCAAAAGCAGCTCTACCTCGCGGCCCTCACCAAACCGCAAGGCATGATC
TTAATGACCGGCCCAACCGGCAGCGGCAAAACTGTGTCGCTCTACACCGGATTACGCATTCTCAATACCGAACAAGTGAA
CATCTCCACCGCCGAAGATCCGGTGGAAATCAACCTGCCCGGCATCAATCAGGTGCAAGTTCAACCCAAAATCGGCTTTG
GCTTTGCACAAGCGCTGCGCTCGTTTCTGCGTCAAGATCCCGATGTGGTGATGGTGGGCGAAATCCGCGATCTAGAAACG
GCAGAAATCGCCATTAAGGCCGCGCAAACCGGGCACTTGGTGCTCTCAACCTTGCACACCAACTCGGCCGCGGAAACCGT
GATTCGTTTAGCCAACATGGGCATTGAACCCTTTAACCTTGCCTCATCATTGAGCCTGATCATTGCGCAGCGGCTGGCCC
GCCGTTTGTGCCCGCATTGCAAGGTCGCCATCACGCCCCCAGCTGCGCTGCAGCAGCAATTTGGCTTGCTCGCCGAAGAT
GCCGTGTACGAAGCACATCCATCGGGTTGCACGGAATGCACCAATGGGTATAGCGGCCGCGTGGGCATTTATGAAGTGAT
GGCGTTTCATCCCGCCTTGGCGGACGCGGTGATGCAGCGCGCCAGCATTCATCAAATCGAAACCATTGCCCAACAGCACG
GCATGCAACCCCTGCTGCAATCGGGGATTGAAAAACTGCGCGCGGGCATCACCAGTTTTAGTGAATTACAGCGAGTACTC
TACTTTTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(39-142)

Type II/IV secretion system protein (T2SSE)

(188-455)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

82.384

100

0.824
  pilB Vibrio campbellii strain DS40M4

71.886

100

0.719
  pilB Vibrio parahaemolyticus RIMD 2210633

71.886

100

0.719
  pilB Legionella pneumophila strain ERS1305867

52.519

91.815

0.482
  pilB Acinetobacter baumannii D1279779

51.06

92.349

0.472
  pilB Acinetobacter baylyi ADP1

50.097

91.993

0.461
  pilF Neisseria gonorrhoeae MS11

44.858

100

0.45
  pilB Haemophilus influenzae 86-028NP

39.658

93.772

0.372
  pilB/pilB1 Synechocystis sp. PCC 6803

37.636

97.865

0.368
  pilF Thermus thermophilus HB27

36.655

100

0.367

Multiple sequence alignment