Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ALVIN_RS13065 Genome accession   NC_013851
Coordinates   2954149..2955303 (+) Length   384 a.a.
NCBI ID   WP_012971795.1    Uniprot ID   D3RPD7
Organism   Allochromatium vinosum DSM 180     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2949149..2960303
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ALVIN_RS13045 (Alvin_2613) - 2950008..2951189 (+) 1182 WP_012971791.1 response regulator -
  ALVIN_RS13050 (Alvin_2614) purK 2951337..2952491 (+) 1155 WP_012971792.1 5-(carboxyamino)imidazole ribonucleotide synthase -
  ALVIN_RS13055 (Alvin_2615) purE 2952488..2953003 (+) 516 WP_012971793.1 5-(carboxyamino)imidazole ribonucleotide mutase -
  ALVIN_RS13060 (Alvin_2616) pilT 2953080..2954117 (+) 1038 WP_012971794.1 type IV pilus twitching motility protein PilT Machinery gene
  ALVIN_RS13065 (Alvin_2617) pilU 2954149..2955303 (+) 1155 WP_012971795.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ALVIN_RS13070 (Alvin_2618) - 2955313..2956386 (-) 1074 WP_012971796.1 HD domain-containing phosphohydrolase -
  ALVIN_RS13075 (Alvin_2619) - 2956440..2957687 (-) 1248 WP_223295219.1 PP2C family protein-serine/threonine phosphatase -
  ALVIN_RS18340 - 2957689..2958375 (-) 687 WP_407637041.1 methyl-accepting chemotaxis protein -
  ALVIN_RS18345 - 2958915..2959910 (-) 996 Protein_2612 MCP four helix bundle domain-containing protein -

Sequence

Protein


Download         Length: 384 a.a.        Molecular weight: 42951.25 Da        Isoelectric Point: 6.1224

>NTDB_id=36276 ALVIN_RS13065 WP_012971795.1 2954149..2955303(+) (pilU) [Allochromatium vinosum DSM 180]
MDLKRALEQLLSALVEKKGSDLFITAGWPPSIKIDGTIYPATKQPLTADQAQGMVYEIMNERQRAEFEDTKECQFALDRA
PLGRFRVSAFVQRDAVGMVLRRIETRIPTLEELKLPSVLRDLAMTKRGLVIFVGATGTGKSTSLAALIGYRNHHSSGHII
TVEDPIEYVHEHDGCIITQREVGVDTESFEVALRNTLRQAPDVILIGEIRTRQTMEYAITFAETGHLVLATLHANNANQA
MDRVINFFPEESRSQMLMDLSLNLKGIVAQQLAPRKNGQGRRAVIEILLNTPLAADLIRLGEVHKLKDLMKRSGEQGMIT
FDQALFNLYQEGEISYEDALKYADSANEVRLMIKLQSGMTPSSTTDQGIDAMSLVRDEDPRNKR

Nucleotide


Download         Length: 1155 bp        

>NTDB_id=36276 ALVIN_RS13065 WP_012971795.1 2954149..2955303(+) (pilU) [Allochromatium vinosum DSM 180]
ATGGATCTGAAGCGCGCGCTGGAACAACTCCTGAGTGCCCTGGTCGAGAAAAAGGGCTCGGACCTCTTCATCACCGCCGG
CTGGCCGCCGAGCATCAAGATCGACGGCACCATCTATCCGGCGACCAAGCAGCCGCTCACGGCCGATCAGGCGCAGGGCA
TGGTCTACGAGATCATGAACGAGCGCCAGCGCGCCGAATTCGAAGACACCAAGGAGTGTCAGTTCGCGCTCGACCGCGCC
CCGCTCGGACGCTTCCGTGTCAGCGCCTTCGTCCAGCGCGACGCCGTCGGCATGGTGCTGCGGCGCATCGAGACCCGCAT
CCCGACCCTGGAGGAACTCAAGCTCCCCTCGGTGCTGCGCGATCTGGCCATGACCAAGCGCGGTCTGGTGATCTTCGTCG
GCGCCACCGGCACGGGTAAATCGACCTCGCTCGCGGCCCTGATCGGCTACCGCAACCATCACAGCTCGGGCCACATCATC
ACGGTCGAGGACCCGATCGAGTACGTCCACGAGCACGACGGCTGCATCATCACCCAGCGCGAGGTCGGCGTGGACACCGA
GTCCTTCGAGGTCGCCCTGCGCAACACCCTGCGTCAGGCCCCGGACGTCATCCTGATCGGCGAGATCCGCACCCGCCAGA
CCATGGAGTACGCCATCACCTTCGCCGAGACCGGCCATCTGGTGCTCGCCACCCTGCACGCCAACAACGCCAATCAGGCC
ATGGATCGCGTCATCAACTTCTTCCCCGAGGAATCGCGCAGCCAGATGCTCATGGATCTCTCGCTCAACCTCAAGGGCAT
CGTCGCCCAGCAACTGGCGCCGCGCAAGAACGGCCAGGGCCGGCGTGCGGTGATCGAGATCCTGCTCAACACGCCGCTGG
CGGCCGATCTCATCCGCTTGGGCGAGGTGCACAAGCTCAAGGATCTGATGAAGCGTTCGGGCGAACAGGGCATGATCACC
TTCGATCAGGCGCTGTTCAATCTCTATCAGGAAGGCGAGATCAGCTACGAGGATGCCCTGAAGTACGCCGACTCGGCCAA
CGAGGTGCGGCTGATGATCAAGCTACAGAGCGGTATGACGCCGAGCAGTACGACGGATCAGGGCATCGACGCCATGAGTC
TGGTCAGGGACGAAGACCCCCGGAACAAACGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(117-274)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB D3RPD7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

69.516

91.406

0.635
  pilU Acinetobacter baylyi ADP1

62.824

90.365

0.568
  pilU Vibrio cholerae strain A1552

58.953

94.531

0.557
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.5

93.75

0.398
  pilT Legionella pneumophila strain Lp02

43.027

87.76

0.378
  pilT Legionella pneumophila strain ERS1305867

43.027

87.76

0.378
  pilT Acinetobacter baylyi ADP1

41.593

88.281

0.367
  pilT Pseudomonas aeruginosa PAK

41.246

87.76

0.362

Multiple sequence alignment