Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   GPY01_RS16240 Genome accession   NZ_CP046839
Coordinates   2535076..2535528 (-) Length   150 a.a.
NCBI ID   WP_123161617.1    Uniprot ID   -
Organism   Vibrio cholerae strain 2011EL-1271     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2530076..2540528
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GPY01_RS16220 coaE 2530574..2531182 (-) 609 WP_170921665.1 dephospho-CoA kinase -
  GPY01_RS16225 pilD 2531179..2532054 (-) 876 WP_033931359.1 A24 family peptidase Machinery gene
  GPY01_RS16230 pilC 2532112..2533338 (-) 1227 WP_032471528.1 type II secretion system F family protein Machinery gene
  GPY01_RS16235 pilB 2533381..2535069 (-) 1689 WP_123161616.1 type IV-A pilus assembly ATPase PilB Machinery gene
  GPY01_RS16240 pilA 2535076..2535528 (-) 453 WP_123161617.1 pilin Machinery gene
  GPY01_RS16245 nadC 2535773..2536663 (-) 891 WP_002053528.1 carboxylating nicotinate-nucleotide diphosphorylase -
  GPY01_RS16250 ampD 2536963..2537508 (+) 546 WP_123161618.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  GPY01_RS16255 fldB 2537803..2538321 (-) 519 WP_000690112.1 flavodoxin FldB -
  GPY01_RS16260 xerD 2538459..2539367 (+) 909 WP_001287472.1 site-specific tyrosine recombinase XerD -
  GPY01_RS16265 dsbC 2539384..2540136 (+) 753 WP_000115049.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -

Sequence

Protein


Download         Length: 150 a.a.        Molecular weight: 15834.42 Da        Isoelectric Point: 9.6574

>NTDB_id=356366 GPY01_RS16240 WP_123161617.1 2535076..2535528(-) (pilA) [Vibrio cholerae strain 2011EL-1271]
MKAYKNKLQKGFTLIELMIVVAVIGVLAAIAMPQYQKYVAKSEVASVLATLTGSKTNVEAYTVENGIFPKKGDEIKLGVP
KMPLGEAVFPESPTNDGGTIKFTFNSVASGASSLTAGKNLTLTRTASTGAWKCSTEGTTLDAELLPKNCK

Nucleotide


Download         Length: 453 bp        

>NTDB_id=356366 GPY01_RS16240 WP_123161617.1 2535076..2535528(-) (pilA) [Vibrio cholerae strain 2011EL-1271]
ATGAAAGCGTATAAAAATAAACTACAGAAAGGTTTTACCTTAATTGAATTGATGATTGTGGTGGCAGTGATTGGTGTGTT
GGCAGCGATTGCTATGCCTCAATATCAAAAATATGTTGCTAAAAGTGAGGTAGCCTCTGTTTTAGCAACACTGACTGGCA
GCAAGACCAACGTAGAGGCATATACTGTTGAAAATGGCATTTTTCCCAAAAAAGGCGATGAAATAAAGCTAGGAGTTCCT
AAAATGCCTTTAGGTGAAGCTGTATTCCCTGAGTCACCTACAAATGATGGTGGAACAATCAAATTTACTTTTAATTCTGT
AGCTAGTGGTGCTAGCAGTTTGACAGCTGGAAAAAATTTAACCTTGACACGAACAGCATCTACAGGTGCATGGAAATGTT
CAACTGAAGGTACCACACTTGATGCCGAGCTTTTACCTAAAAATTGTAAATAG

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(7-32)

Pilin (bacterial filament) (Pilin)

(40-150)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Vibrio cholerae strain A1552

46.795

100

0.487
  pilA Vibrio cholerae C6706

46.795

100

0.487
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

46.795

100

0.487
  pilA Pseudomonas aeruginosa PAK

45.395

100

0.46
  pilA Vibrio parahaemolyticus RIMD 2210633

45.865

88.667

0.407
  pilA/pilAI Pseudomonas stutzeri DSM 10701

37.821

100

0.393
  pilA Acinetobacter baumannii strain A118

40.426

94

0.38