Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   CTCNB1_RS03855 Genome accession   NC_013446
Coordinates   854911..856128 (-) Length   405 a.a.
NCBI ID   WP_012837103.1    Uniprot ID   A0A096FFA0
Organism   Comamonas thiooxydans     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 849911..861128
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CTCNB1_RS03825 (CtCNB1_0755) - 850720..851655 (+) 936 WP_039049610.1 ATP-binding protein -
  CTCNB1_RS03830 (CtCNB1_0756) - 851668..852138 (+) 471 WP_012837099.1 NUDIX domain-containing protein -
  CTCNB1_RS03835 (CtCNB1_0757) - 852256..852465 (-) 210 WP_039049611.1 DNA gyrase inhibitor YacG -
  CTCNB1_RS03840 (CtCNB1_0758) zapD 852569..853324 (-) 756 WP_012837101.1 cell division protein ZapD -
  CTCNB1_RS03845 (CtCNB1_0759) coaE 853412..854038 (-) 627 WP_012837102.1 dephospho-CoA kinase -
  CTCNB1_RS03850 (CtCNB1_0760) - 854042..854911 (-) 870 WP_003062192.1 prepilin peptidase -
  CTCNB1_RS03855 (CtCNB1_0761) pilC 854911..856128 (-) 1218 WP_012837103.1 type II secretion system F family protein Machinery gene
  CTCNB1_RS03860 (CtCNB1_0762) pilB 856153..857895 (-) 1743 WP_012837104.1 type IV-A pilus assembly ATPase PilB Machinery gene
  CTCNB1_RS03870 (CtCNB1_0763) - 858149..859078 (-) 930 WP_003062198.1 polyprenyl synthetase family protein -
  CTCNB1_RS03875 (CtCNB1_0764) rplU 859423..859734 (+) 312 WP_003058536.1 50S ribosomal protein L21 -
  CTCNB1_RS03880 (CtCNB1_0765) rpmA 859751..860008 (+) 258 WP_003058534.1 50S ribosomal protein L27 -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44541.27 Da        Isoelectric Point: 9.6163

>NTDB_id=35555 CTCNB1_RS03855 WP_012837103.1 854911..856128(-) (pilC) [Comamonas thiooxydans]
MASAASKGIKEFLFEWEGKDRNGKIVRGETRAGGENQIQAMLRRQGVTPSKIKKRRTRGGKKIKPKDIALFTRQLATMMK
AGVPLLQSFDIVGRGNTNPNVTKLLNDIRSDVETGTSLSAAFRKFPLYFNSLYCNLVEAGEAAGILESLLDRLATYMEKT
EAIKSKIKSALMYPTSVMIVAFVVVTVIMIFVIPAFKEVFTSFGADLPAPTLLVMGISDYFVQYWWLIFGVLGGGIYFFM
QAWKRNERVQQFMDRTILKLPIFGVLIEKSCVARWTRTLSTMFAAGVPLVEALDSVGGASGNYLYRNATDRIQSEVSTGT
SLTVAMANANIFPSMVLQMCAIGEESGAIDHMLGKAADFYESEVDEMVAGLSSLMEPIIIVFLGTLIGGIVVSMYLPIFK
LGQVV

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=35555 CTCNB1_RS03855 WP_012837103.1 854911..856128(-) (pilC) [Comamonas thiooxydans]
ATGGCAAGCGCAGCGTCCAAGGGAATCAAGGAATTTCTCTTCGAGTGGGAAGGCAAGGACCGCAACGGCAAGATCGTTCG
CGGCGAAACTCGTGCCGGCGGAGAAAACCAGATCCAGGCCATGCTGCGCCGCCAGGGCGTGACTCCGTCCAAGATCAAGA
AGCGCAGGACCCGCGGCGGCAAGAAGATCAAGCCCAAGGACATTGCGCTGTTCACGCGCCAGCTGGCCACCATGATGAAG
GCCGGCGTGCCGCTGCTGCAGTCTTTCGACATCGTGGGCCGGGGCAACACCAACCCCAACGTCACCAAGCTGCTCAACGA
CATCCGCTCCGATGTGGAAACCGGCACTTCGCTGAGCGCCGCCTTTCGCAAGTTTCCGCTCTATTTCAACAGCCTCTACT
GCAATCTGGTGGAGGCCGGCGAGGCCGCAGGTATTCTGGAATCGTTGCTGGACCGTCTTGCCACCTATATGGAAAAGACG
GAAGCCATCAAGTCCAAGATCAAGTCGGCGCTGATGTACCCCACGTCGGTCATGATCGTGGCCTTTGTCGTGGTGACCGT
GATCATGATCTTCGTGATCCCTGCCTTCAAGGAGGTCTTCACCTCCTTTGGCGCCGACCTGCCCGCGCCCACGCTGCTGG
TGATGGGCATCAGCGATTACTTTGTCCAGTATTGGTGGCTGATCTTCGGCGTGCTGGGCGGCGGCATCTATTTCTTCATG
CAGGCCTGGAAGCGCAACGAGCGCGTACAGCAGTTCATGGACCGCACCATACTCAAGCTGCCCATCTTCGGCGTGCTGAT
CGAGAAGTCCTGCGTGGCCCGCTGGACACGTACGCTGTCCACCATGTTTGCCGCCGGCGTGCCGCTGGTCGAGGCGCTGG
ACTCCGTGGGAGGCGCCTCGGGCAACTACCTCTACAGAAACGCCACCGACAGGATTCAGTCGGAAGTCTCCACGGGCACC
AGCCTGACCGTGGCCATGGCCAATGCCAACATCTTCCCTTCCATGGTGCTGCAGATGTGTGCCATCGGCGAGGAATCGGG
CGCCATCGACCATATGCTGGGCAAGGCAGCCGATTTCTATGAAAGCGAAGTCGACGAAATGGTGGCGGGCCTCTCCAGCC
TGATGGAGCCCATCATCATCGTCTTCCTGGGCACCTTGATCGGCGGCATCGTGGTGTCCATGTATCTGCCTATCTTCAAG
CTGGGTCAAGTGGTCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)

Type II secretion system (T2SS), protein F (T2SSF)

(275-397)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A096FFA0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

53.713

99.753

0.536
  pilG Neisseria gonorrhoeae MS11

51.889

98.025

0.509
  pilG Neisseria meningitidis 44/76-A

51.637

98.025

0.506
  pilC Legionella pneumophila strain ERS1305867

50.758

97.778

0.496
  pilC Acinetobacter baylyi ADP1

49.749

98.272

0.489
  pilC Acinetobacter baumannii D1279779

48.363

98.025

0.474
  pilC Vibrio cholerae strain A1552

40.302

98.025

0.395
  pilC Vibrio campbellii strain DS40M4

38.06

99.259

0.378
  pilC Thermus thermophilus HB27

36.634

99.753

0.365

Multiple sequence alignment