Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   CTCNB1_RS03860 Genome accession   NC_013446
Coordinates   856153..857895 (-) Length   580 a.a.
NCBI ID   WP_012837104.1    Uniprot ID   -
Organism   Comamonas thiooxydans     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 851153..862895
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CTCNB1_RS03830 (CtCNB1_0756) - 851668..852138 (+) 471 WP_012837099.1 NUDIX domain-containing protein -
  CTCNB1_RS03835 (CtCNB1_0757) - 852256..852465 (-) 210 WP_039049611.1 DNA gyrase inhibitor YacG -
  CTCNB1_RS03840 (CtCNB1_0758) zapD 852569..853324 (-) 756 WP_012837101.1 cell division protein ZapD -
  CTCNB1_RS03845 (CtCNB1_0759) coaE 853412..854038 (-) 627 WP_012837102.1 dephospho-CoA kinase -
  CTCNB1_RS03850 (CtCNB1_0760) - 854042..854911 (-) 870 WP_003062192.1 prepilin peptidase -
  CTCNB1_RS03855 (CtCNB1_0761) pilC 854911..856128 (-) 1218 WP_012837103.1 type II secretion system F family protein Machinery gene
  CTCNB1_RS03860 (CtCNB1_0762) pilB 856153..857895 (-) 1743 WP_012837104.1 type IV-A pilus assembly ATPase PilB Machinery gene
  CTCNB1_RS03870 (CtCNB1_0763) - 858149..859078 (-) 930 WP_003062198.1 polyprenyl synthetase family protein -
  CTCNB1_RS03875 (CtCNB1_0764) rplU 859423..859734 (+) 312 WP_003058536.1 50S ribosomal protein L21 -
  CTCNB1_RS03880 (CtCNB1_0765) rpmA 859751..860008 (+) 258 WP_003058534.1 50S ribosomal protein L27 -
  CTCNB1_RS03885 (CtCNB1_0766) cgtA 860165..861280 (+) 1116 WP_003062200.1 Obg family GTPase CgtA -
  CTCNB1_RS03890 (CtCNB1_0767) proB 861391..862530 (+) 1140 WP_012837105.1 glutamate 5-kinase -

Sequence

Protein


Download         Length: 580 a.a.        Molecular weight: 63102.33 Da        Isoelectric Point: 5.1537

>NTDB_id=35556 CTCNB1_RS03860 WP_012837104.1 856153..857895(-) (pilB) [Comamonas thiooxydans]
MAAADTLQKNTPAPIAIPGLGRALISAGKVSQQAAENAAKKAATGKTPFITELSQSGEISALEIAETISMMFSTPLLDLN
AIDVQQLPRELLDPKISNAYRVLALSKRGNRLTIATADPTQQEAAEQIKFTTQLLVDWVVVEADKLQKLVEQTGKSVSES
LDSYSSSGDFDFDDVKIEDAPEEKDNAVGAEVEDAPVVKFLHKMLLDAFNMRASDLHFEPYEHNYRVRFRIDGELREIAS
PPIAIKDKLASRIKVISRLDISEKRVPQDGRMKLKVGPDRVIDFRVSTLPTLFGEKIVIRILDPSSAKMGIDALGYEPEE
KERLLKAINRPYGMILVTGPTGSGKTVSLYTCLNLLNQPGVNIATAEDPSEINMPGVNQVNVNEKAGLTFAAALKSFLRQ
DPDIIMVGEIRDLETADISIKAAQTGHLVLSTLHTNDAPTTLTRMRNMGIAPFNIASSVILITAQRLARRLCAQCKEPAD
IPHDALIDAGYDEAELDGSWVSYKPVGCPACNNGYKGRVGIYQVMPISEEIQRIILRDGSALEIAAQAKAEGVRSLRGSG
LHKVKLGLTSLEEVLAVTNE

Nucleotide


Download         Length: 1743 bp        

>NTDB_id=35556 CTCNB1_RS03860 WP_012837104.1 856153..857895(-) (pilB) [Comamonas thiooxydans]
ATGGCTGCTGCCGACACTCTGCAAAAGAACACTCCTGCGCCCATTGCCATCCCAGGTTTAGGCAGGGCCCTGATTTCTGC
AGGAAAGGTTTCGCAGCAAGCCGCCGAGAACGCAGCCAAGAAAGCCGCCACAGGCAAGACCCCGTTCATCACCGAGCTCA
GCCAGTCTGGTGAAATCAGCGCACTGGAAATTGCCGAAACCATTTCCATGATGTTCAGCACTCCGCTGCTGGACCTCAAC
GCCATCGACGTCCAGCAACTTCCGCGCGAGCTGCTGGACCCGAAAATCAGCAACGCCTATCGCGTGCTGGCACTCAGCAA
GCGCGGCAATCGGCTCACCATCGCCACGGCAGACCCTACTCAGCAGGAAGCAGCCGAGCAGATCAAGTTCACGACTCAGT
TGCTGGTGGACTGGGTGGTTGTCGAGGCCGACAAGCTGCAAAAGCTCGTCGAGCAGACCGGCAAGAGCGTCAGCGAATCC
CTGGACTCCTATTCCAGCTCAGGAGACTTCGACTTTGACGACGTCAAGATCGAGGATGCCCCCGAGGAAAAAGACAATGC
CGTCGGCGCCGAGGTCGAGGATGCGCCGGTCGTCAAGTTTCTGCACAAGATGCTGCTGGACGCCTTCAATATGCGCGCGT
CCGACTTGCACTTCGAGCCCTACGAACACAATTACCGCGTGCGCTTTCGCATCGACGGCGAGCTGCGCGAGATTGCCTCT
CCTCCTATCGCGATCAAGGACAAGCTGGCTTCGCGCATCAAGGTGATCTCGCGCCTGGACATCTCGGAGAAACGCGTTCC
GCAAGACGGCCGCATGAAGCTCAAGGTCGGCCCCGATCGCGTCATCGACTTTCGTGTCAGCACCTTGCCCACGCTGTTTG
GCGAAAAGATCGTGATCCGTATTCTGGACCCGAGCTCGGCCAAGATGGGCATCGATGCCCTCGGCTACGAACCCGAGGAA
AAAGAGCGCCTGCTCAAGGCCATCAACCGCCCCTACGGCATGATTTTGGTTACCGGCCCCACGGGCTCGGGCAAGACCGT
GTCGCTCTACACCTGCCTGAACCTGCTCAACCAGCCGGGCGTGAACATTGCCACCGCCGAAGACCCTTCCGAAATCAACA
TGCCCGGCGTCAACCAGGTGAATGTGAACGAGAAGGCCGGCCTGACTTTTGCCGCTGCGCTCAAGTCCTTTCTGCGCCAG
GACCCGGACATCATCATGGTCGGTGAAATCCGTGACCTGGAAACCGCCGACATCTCGATCAAGGCCGCCCAGACCGGGCA
CTTGGTGCTGTCCACGCTGCACACCAATGACGCTCCGACCACGCTGACGCGCATGCGCAATATGGGCATCGCCCCTTTCA
ATATTGCCTCCAGCGTGATCCTGATCACCGCCCAGCGTCTGGCGCGACGCCTGTGCGCGCAGTGCAAGGAGCCCGCCGAC
ATACCGCACGACGCCCTGATCGATGCCGGCTACGACGAAGCCGAACTCGACGGCAGCTGGGTCAGCTACAAGCCTGTCGG
CTGCCCTGCCTGCAACAACGGCTACAAGGGACGTGTCGGTATTTATCAGGTGATGCCCATCAGCGAAGAAATTCAGCGCA
TCATCCTGCGTGACGGCAGCGCCCTGGAGATTGCCGCACAGGCCAAGGCCGAGGGAGTACGCTCTCTGCGCGGCTCGGGC
CTGCATAAAGTCAAGCTGGGTCTGACCTCACTCGAAGAAGTGCTGGCGGTCACCAACGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(203-470)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(55-153)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

54.53

98.966

0.54
  pilB Acinetobacter baumannii D1279779

55.203

97.759

0.54
  pilF Neisseria gonorrhoeae MS11

52.473

97.586

0.512
  pilB Legionella pneumophila strain ERS1305867

51.06

97.586

0.498
  pilB Vibrio cholerae strain A1552

46.898

94.483

0.443
  pilB Vibrio campbellii strain DS40M4

44.029

96.724

0.426
  pilB Vibrio parahaemolyticus RIMD 2210633

46.008

90.69

0.417
  pilF Thermus thermophilus HB27

37.755

100

0.383
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.421

98.276

0.378

Multiple sequence alignment