Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   GPY21_RS11170 Genome accession   NZ_CP046754
Coordinates   483173..483625 (-) Length   150 a.a.
NCBI ID   WP_005493986.1    Uniprot ID   -
Organism   Vibrio parahaemolyticus strain 2015AW-0174     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 478173..488625
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GPY21_RS11150 coaE 478690..479304 (-) 615 WP_005493969.1 dephospho-CoA kinase -
  GPY21_RS11155 pilD 479305..480174 (-) 870 WP_005493974.1 A24 family peptidase Machinery gene
  GPY21_RS11160 pilC 480239..481462 (-) 1224 WP_005493981.1 type II secretion system F family protein Machinery gene
  GPY21_RS11165 pilB 481486..483171 (-) 1686 WP_005493983.1 type IV-A pilus assembly ATPase PilB Machinery gene
  GPY21_RS11170 pilA 483173..483625 (-) 453 WP_005493986.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  GPY21_RS11175 nadC 483889..484776 (-) 888 WP_005493988.1 carboxylating nicotinate-nucleotide diphosphorylase -
  GPY21_RS11180 ampD 484869..485420 (+) 552 WP_005484832.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  GPY21_RS11185 pdhR 485826..486593 (+) 768 WP_005462576.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 150 a.a.        Molecular weight: 15708.99 Da        Isoelectric Point: 7.1316

>NTDB_id=354919 GPY21_RS11170 WP_005493986.1 483173..483625(-) (pilA) [Vibrio parahaemolyticus strain 2015AW-0174]
MKHSKQKKQQGFTLIELMIVVGIIGIISALAVPAYKSYVLKTEANTAVGVPRALLANVDLFVQEKGKYPDSTQTADLAAI
GAAIDMSAMGTLAITPDADGSEYGDIEFTIGSNASLSGKKVTFARSTNGWKCTHDTGQDLKGCATTPATP

Nucleotide


Download         Length: 453 bp        

>NTDB_id=354919 GPY21_RS11170 WP_005493986.1 483173..483625(-) (pilA) [Vibrio parahaemolyticus strain 2015AW-0174]
ATGAAACACAGTAAACAGAAAAAACAGCAAGGTTTTACCTTGATTGAATTGATGATTGTGGTTGGGATTATTGGGATTAT
AAGTGCATTAGCTGTACCAGCTTATAAAAGCTATGTACTAAAAACCGAAGCTAATACTGCTGTGGGCGTGCCAAGAGCCT
TGTTAGCAAACGTAGACCTCTTCGTCCAAGAAAAAGGTAAATACCCTGATTCTACTCAAACTGCCGATCTTGCAGCTATC
GGAGCTGCTATAGATATGAGTGCGATGGGAACTCTTGCTATCACTCCTGACGCAGACGGTTCTGAGTACGGGGACATTGA
GTTTACCATTGGCTCTAATGCTTCATTGAGTGGCAAAAAGGTTACGTTTGCTCGCTCAACTAACGGTTGGAAGTGTACGC
ATGATACAGGTCAAGACCTTAAAGGCTGTGCTACCACTCCAGCAACTCCTTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(7-31)

Pilin (bacterial filament) (Pilin)

(56-139)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Pseudomonas aeruginosa PAK

37.838

98.667

0.373
  pilA Vibrio parahaemolyticus RIMD 2210633

42.105

88.667

0.373

Multiple sequence alignment