Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   E2K93_RS10805 Genome accession   NZ_CP038493
Coordinates   2505417..2506658 (-) Length   413 a.a.
NCBI ID   WP_323368245.1    Uniprot ID   -
Organism   Thalassotalea sp. HSM 43     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2500417..2511658
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E2K93_RS10775 (E2K93_10775) - 2500762..2501646 (+) 885 WP_135439096.1 LysR substrate-binding domain-containing protein -
  E2K93_RS10780 (E2K93_10780) - 2501879..2502421 (+) 543 WP_135439097.1 hypothetical protein -
  E2K93_RS10785 (E2K93_10785) yacG 2502572..2502808 (-) 237 WP_135439098.1 DNA gyrase inhibitor YacG -
  E2K93_RS10790 (E2K93_10790) zapD 2502983..2503729 (-) 747 WP_135439099.1 cell division protein ZapD -
  E2K93_RS10795 (E2K93_10795) coaE 2503833..2504444 (-) 612 WP_135439100.1 dephospho-CoA kinase -
  E2K93_RS10800 (E2K93_10800) pilD 2504445..2505332 (-) 888 WP_135439101.1 prepilin peptidase Machinery gene
  E2K93_RS10805 (E2K93_10805) pilC 2505417..2506658 (-) 1242 WP_323368245.1 type II secretion system F family protein Machinery gene
  E2K93_RS10810 (E2K93_10810) pilB 2506697..2508400 (-) 1704 WP_416316110.1 type IV-A pilus assembly ATPase PilB Machinery gene
  E2K93_RS17790 (E2K93_10815) - 2508517..2508930 (-) 414 WP_323368246.1 pilin -
  E2K93_RS10820 (E2K93_10820) nadC 2509429..2510322 (-) 894 WP_135439103.1 carboxylating nicotinate-nucleotide diphosphorylase -
  E2K93_RS10825 (E2K93_10825) - 2510393..2510914 (-) 522 WP_135439104.1 retropepsin-like aspartic protease family protein -
  E2K93_RS10830 (E2K93_10830) ampD 2510936..2511547 (+) 612 WP_228445274.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -

Sequence

Protein


Download         Length: 413 a.a.        Molecular weight: 44876.78 Da        Isoelectric Point: 7.8492

>NTDB_id=354744 E2K93_RS10805 WP_323368245.1 2505417..2506658(-) (pilC) [Thalassotalea sp. HSM 43]
MAVASSQPKMVKPKPLETFQWEGVNRKGKKIKGELSATNAMELKAQLRKQGITPLKHKKKQKSLFSGDKPVKPADIAVVT
RQIATMLGAGVPLVQTIEMIGKGNDNGNMRKLLGDIGNKVQSGLPLSECLREHPRYFDDLYCDLVESGEQSGALEAIFDR
IAIYKEKAEALKSKIKKALFYPIAVLVIAAVVTSILLIFVVPTFVDIFNDFGAELPAFTTFVVNISDFMVSSWYLMLGAL
IAAGWMFKQAHRRSLDFRDKVDAFVLKVPVIGMILDKAAVARFARTLSTTFAAGVPLIDALDSAAGASGNAVYRDAIQDI
RSEVSAGMQMNLAMRNVNVFPDMVIQMVTIGEESGALDDMLAKVANIYEQEVDDAVDGLTSLLEPLIMAVLGVVIGGLII
AMYLPIFAMGSIL

Nucleotide


Download         Length: 1242 bp        

>NTDB_id=354744 E2K93_RS10805 WP_323368245.1 2505417..2506658(-) (pilC) [Thalassotalea sp. HSM 43]
ATGGCAGTAGCAAGTTCCCAACCGAAAATGGTTAAACCTAAACCTCTTGAAACGTTTCAATGGGAAGGGGTTAACCGTAA
AGGTAAAAAAATCAAAGGTGAGTTATCCGCTACGAATGCAATGGAGCTAAAAGCGCAATTGCGAAAACAAGGTATCACGC
CGTTAAAGCATAAAAAGAAACAAAAATCCTTATTCAGCGGCGACAAGCCGGTCAAGCCAGCCGACATTGCGGTTGTAACT
CGACAAATTGCCACCATGTTAGGTGCGGGTGTTCCTTTGGTGCAAACCATTGAAATGATTGGTAAAGGTAATGATAACGG
CAATATGCGCAAACTGCTAGGAGACATAGGCAATAAAGTTCAATCTGGCCTGCCGCTATCAGAGTGTTTAAGAGAACACC
CAAGATATTTTGACGATTTATATTGTGACTTGGTTGAATCCGGTGAACAATCGGGTGCGTTAGAAGCCATTTTTGACCGG
ATTGCTATCTATAAAGAAAAAGCCGAAGCCCTAAAATCAAAAATTAAAAAAGCACTATTCTACCCCATTGCTGTATTAGT
CATTGCTGCTGTTGTTACATCAATATTGTTAATCTTTGTTGTACCGACGTTCGTCGATATATTTAATGATTTCGGTGCTG
AGCTGCCGGCATTTACGACTTTTGTGGTAAACATTTCAGACTTTATGGTGTCATCTTGGTACCTAATGCTTGGAGCATTA
ATTGCGGCGGGTTGGATGTTTAAACAAGCCCACCGAAGAAGTTTAGATTTTCGAGATAAAGTAGATGCGTTTGTATTAAA
AGTACCTGTAATAGGTATGATCTTGGACAAAGCTGCGGTTGCTCGATTTGCACGTACATTATCAACAACGTTTGCCGCAG
GCGTACCTTTAATTGATGCATTAGATTCAGCAGCAGGCGCATCAGGTAATGCCGTCTACCGAGATGCGATTCAAGATATC
CGCAGTGAGGTTTCAGCCGGTATGCAAATGAATTTGGCGATGCGAAACGTAAACGTATTTCCGGATATGGTGATCCAAAT
GGTCACTATCGGAGAAGAATCCGGCGCTCTAGATGATATGCTCGCTAAGGTTGCAAACATTTACGAGCAAGAAGTCGATG
ATGCCGTTGATGGCTTGACCTCATTATTAGAACCTCTAATTATGGCCGTATTAGGGGTTGTGATTGGTGGTCTAATCATC
GCTATGTACCTACCTATTTTCGCTATGGGTTCGATTCTATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(283-405)

Type II secretion system (T2SS), protein F (T2SSF)

(80-202)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

61.772

95.642

0.591
  pilC Acinetobacter baylyi ADP1

56.188

97.821

0.55
  pilC Acinetobacter baumannii D1279779

55.446

97.821

0.542
  pilC Legionella pneumophila strain ERS1305867

53.015

96.368

0.511
  pilC Vibrio cholerae strain A1552

44.853

98.789

0.443
  pilC Vibrio campbellii strain DS40M4

43.902

99.274

0.436
  pilG Neisseria meningitidis 44/76-A

43.392

97.094

0.421
  pilG Neisseria gonorrhoeae MS11

43.142

97.094

0.419

Multiple sequence alignment