Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   E2K93_RS10810 Genome accession   NZ_CP038493
Coordinates   2506697..2508400 (-) Length   567 a.a.
NCBI ID   WP_416316110.1    Uniprot ID   -
Organism   Thalassotalea sp. HSM 43     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2501697..2513400
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E2K93_RS10780 (E2K93_10780) - 2501879..2502421 (+) 543 WP_135439097.1 hypothetical protein -
  E2K93_RS10785 (E2K93_10785) yacG 2502572..2502808 (-) 237 WP_135439098.1 DNA gyrase inhibitor YacG -
  E2K93_RS10790 (E2K93_10790) zapD 2502983..2503729 (-) 747 WP_135439099.1 cell division protein ZapD -
  E2K93_RS10795 (E2K93_10795) coaE 2503833..2504444 (-) 612 WP_135439100.1 dephospho-CoA kinase -
  E2K93_RS10800 (E2K93_10800) pilD 2504445..2505332 (-) 888 WP_135439101.1 prepilin peptidase Machinery gene
  E2K93_RS10805 (E2K93_10805) pilC 2505417..2506658 (-) 1242 WP_323368245.1 type II secretion system F family protein Machinery gene
  E2K93_RS10810 (E2K93_10810) pilB 2506697..2508400 (-) 1704 WP_416316110.1 type IV-A pilus assembly ATPase PilB Machinery gene
  E2K93_RS17790 (E2K93_10815) - 2508517..2508930 (-) 414 WP_323368246.1 pilin -
  E2K93_RS10820 (E2K93_10820) nadC 2509429..2510322 (-) 894 WP_135439103.1 carboxylating nicotinate-nucleotide diphosphorylase -
  E2K93_RS10825 (E2K93_10825) - 2510393..2510914 (-) 522 WP_135439104.1 retropepsin-like aspartic protease family protein -
  E2K93_RS10830 (E2K93_10830) ampD 2510936..2511547 (+) 612 WP_228445274.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  E2K93_RS10835 (E2K93_10835) ampE 2511634..2512479 (+) 846 WP_135439105.1 regulatory signaling modulator protein AmpE -

Sequence

Protein


Download         Length: 567 a.a.        Molecular weight: 62176.27 Da        Isoelectric Point: 4.9301

>NTDB_id=354745 E2K93_RS10810 WP_416316110.1 2506697..2508400(-) (pilB) [Thalassotalea sp. HSM 43]
MPMKGLHRQSSILSAVVRSELLEKDKAAAIFEQCQKKQMSLVSFLIEKEKVSSIALIKVLSKSFGIPLVNLKSLDLNALP
EGLRNEKLVKKHNALPLYLRGGLLHVAVSDPTNIDALEEFQFNTGHNTEMVLADDKALRDTVEGILEDENSALELTDLDA
DELSDIDVDDTEKEEEQGSGENDAPIVVYINKILLDAIKKGASDLHFEPFETKFRIRFRVDGILTEVATPPVNLANRMTA
RLKVMSKLDIAERRVPQDGRIKLAITKSKSIDFRVSTLPTMWGEKVVMRILDSSSAKLGIDILGYEEEQKELYLDALSKP
QGMILVTGPTGSGKTVSLYTGLNILNTEERNISTAEDPVEINLEGVNQVQINNRAGLTFAEALKSFLRQDPDIVMVGEIR
DLETAEIAIKAAQTGHLVLSTLHTNSAAETLTRLLNMGVPSYNVASSVSLIIAQRLARRLCNHCKEVDDISEQALIELGF
DAAEVSDITIYKPKGCDECTKGYKGRVGIYEVMKISTANAKLIMEGGSSLDIAQQAQSEGYNNLRQSALLKAKAGVTSLA
EVSRVTA

Nucleotide


Download         Length: 1704 bp        

>NTDB_id=354745 E2K93_RS10810 WP_416316110.1 2506697..2508400(-) (pilB) [Thalassotalea sp. HSM 43]
ATACCAATGAAAGGACTCCACCGACAATCAAGCATCCTTTCCGCTGTAGTTCGTAGTGAATTGCTAGAGAAAGACAAAGC
TGCGGCAATATTTGAACAATGCCAGAAAAAGCAAATGTCTCTTGTCTCTTTTTTAATAGAAAAAGAAAAGGTTTCATCCA
TTGCTTTAATAAAAGTATTATCGAAGAGCTTTGGTATCCCTTTAGTCAATTTAAAATCACTTGATTTGAATGCCCTACCT
GAAGGTCTTCGTAACGAAAAGTTAGTTAAAAAGCATAATGCTCTACCTTTATATTTGCGCGGTGGTTTACTGCACGTTGC
TGTCTCCGACCCTACCAATATAGATGCCCTAGAAGAGTTCCAGTTTAATACCGGCCACAATACGGAAATGGTGTTGGCTG
ATGATAAAGCGCTTCGCGATACGGTTGAAGGTATACTCGAAGATGAAAATTCCGCATTAGAATTAACCGATTTAGACGCC
GATGAATTAAGTGATATCGATGTTGATGACACAGAAAAAGAAGAAGAGCAAGGTAGCGGCGAAAACGACGCGCCTATCGT
TGTTTACATCAATAAAATTTTGCTAGACGCCATTAAAAAAGGTGCTTCGGATCTACACTTTGAACCATTTGAAACAAAAT
TCAGAATCCGTTTTCGTGTAGATGGTATTTTAACCGAAGTTGCAACTCCTCCAGTAAACCTTGCAAATCGCATGACTGCT
CGTTTAAAAGTGATGTCAAAACTTGATATCGCCGAACGCCGCGTACCGCAAGATGGTCGTATTAAACTTGCTATTACAAA
GAGTAAATCCATTGATTTTCGTGTATCTACATTACCTACTATGTGGGGTGAAAAAGTGGTTATGCGTATTCTCGATAGCT
CAAGTGCAAAACTTGGTATCGATATTTTAGGTTATGAGGAAGAGCAAAAAGAGCTTTATTTAGATGCCTTATCAAAGCCG
CAAGGTATGATACTGGTTACTGGCCCTACCGGTTCCGGTAAAACTGTATCGCTTTATACTGGCTTAAATATTCTTAATAC
CGAAGAGCGAAATATATCCACCGCAGAGGATCCGGTTGAAATTAACCTTGAGGGTGTTAACCAGGTGCAAATTAACAATC
GAGCTGGCTTAACCTTTGCTGAAGCACTGAAATCATTCCTGCGTCAAGATCCAGATATCGTCATGGTTGGTGAAATTCGA
GATTTAGAAACCGCTGAAATTGCCATCAAAGCGGCACAAACCGGTCACTTGGTATTATCAACTCTGCATACAAACTCAGC
TGCTGAAACACTAACCCGTTTATTAAATATGGGGGTACCATCTTATAACGTTGCAAGTTCAGTAAGCTTAATCATCGCCC
AACGTCTTGCACGCCGTTTGTGTAATCACTGTAAAGAAGTGGATGACATTTCTGAGCAAGCGTTAATAGAACTCGGTTTT
GATGCTGCCGAAGTATCAGACATTACCATATACAAACCCAAAGGGTGTGATGAATGCACCAAAGGCTACAAAGGCCGTGT
CGGTATTTATGAGGTAATGAAAATAAGTACCGCAAATGCCAAACTGATTATGGAAGGTGGCAGTTCACTTGATATTGCCC
AACAGGCGCAAAGTGAAGGTTATAATAACCTGCGCCAGTCGGCATTGCTAAAAGCCAAAGCAGGCGTTACTAGCCTTGCC
GAAGTTAGCCGTGTAACCGCATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(45-147)

Type II/IV secretion system protein (T2SSE)

(192-459)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

56.042

100

0.564
  pilB Legionella pneumophila strain ERS1305867

54.69

99.647

0.545
  pilB Acinetobacter baylyi ADP1

56.852

95.238

0.541
  pilB Vibrio cholerae strain A1552

52.773

98.589

0.52
  pilB Vibrio parahaemolyticus RIMD 2210633

52.05

98.942

0.515
  pilB Vibrio campbellii strain DS40M4

51.865

99.295

0.515
  pilF Neisseria gonorrhoeae MS11

47.67

98.413

0.469
  pilB/pilB1 Synechocystis sp. PCC 6803

40.462

91.534

0.37
  pilF Thermus thermophilus HB27

35.965

100

0.362

Multiple sequence alignment