Detailed information NTG0354743

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: E2K93_RS10800
• Genome accession: NZ_CP038493
• Coordinates: 2504445..2505332 (-)
• Length: 295 a.a.
• NCBI ID: WP_135439101.1
• Uniprot ID: A0A4P7JRH8
• Organism: Thalassotalea sp. HSM 43
• Function: type IV pilus biogenesis and function (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2499445..2510332

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
E2K93_RS10770 (E2K93_10770)	-	2499765..2500280 (-)	516	WP_135439095.1	DUF3465 domain-containing protein	-
E2K93_RS10775 (E2K93_10775)	-	2500762..2501646 (+)	885	WP_135439096.1	LysR substrate-binding domain-containing protein	-
E2K93_RS10780 (E2K93_10780)	-	2501879..2502421 (+)	543	WP_135439097.1	hypothetical protein	-
E2K93_RS10785 (E2K93_10785)	yacG	2502572..2502808 (-)	237	WP_135439098.1	DNA gyrase inhibitor YacG	-
E2K93_RS10790 (E2K93_10790)	zapD	2502983..2503729 (-)	747	WP_135439099.1	cell division protein ZapD	-
E2K93_RS10795 (E2K93_10795)	coaE	2503833..2504444 (-)	612	WP_135439100.1	dephospho-CoA kinase	-
E2K93_RS10800 (E2K93_10800)	pilD	2504445..2505332 (-)	888	WP_135439101.1	prepilin peptidase	Machinery gene
E2K93_RS10805 (E2K93_10805)	pilC	2505417..2506658 (-)	1242	WP_323368245.1	type II secretion system F family protein	Machinery gene
E2K93_RS10810 (E2K93_10810)	pilB	2506697..2508400 (-)	1704	WP_416316110.1	type IV-A pilus assembly ATPase PilB	Machinery gene
E2K93_RS17790 (E2K93_10815)	-	2508517..2508930 (-)	414	WP_323368246.1	pilin	-
E2K93_RS10820 (E2K93_10820)	nadC	2509429..2510322 (-)	894	WP_135439103.1	carboxylating nicotinate-nucleotide diphosphorylase	-

Sequence

Protein

• Length: 295 a.a.
• Molecular weight: 32760.13 Da
• Isoelectric Point: 6.2260

>NTDB_id=354743 E2K93_RS10800 WP_135439101.1 2504445..2505332(-) (pilD) [Thalassotalea sp. HSM 43]
MLSDLTTLFEQSPGFYYSFALILGLIIGSFLNVVIFRLPKMIEAEWDCECREFLADALKDSAKDKEPQTVTLSKPASTCP
KCGHKIKVWENIPVISWLLLRGKCSSCANPISARYPIIETITGISTVVIAMHFGVSWQAAVMMVLSWCLITLTMIDFDKM
ILPDQITLPLVWIGLILGLNNVFVSLDQAVIGAIAGYMSLWSIYWLFKIVTGKEGMGYGDFKLFAVFGAWFGWQVLPMLI
LMASLVGAIVGISLMVFKSHEGSKPIPFGPYLAVAGWITALWGEGIWAWYMGMMV

Nucleotide

• Length: 888 bp

>NTDB_id=354743 E2K93_RS10800 WP_135439101.1 2504445..2505332(-) (pilD) [Thalassotalea sp. HSM 43]
GTGCTTTCCGACTTAACCACTTTATTTGAACAATCCCCAGGTTTTTATTATTCATTTGCATTAATCCTTGGCCTTATCAT
TGGCAGTTTTCTAAATGTGGTAATCTTTCGTTTACCTAAAATGATTGAGGCTGAATGGGATTGCGAATGCCGTGAATTTT
TAGCGGATGCTTTGAAAGATTCAGCTAAGGACAAAGAGCCACAAACAGTGACTCTTTCAAAGCCCGCTTCGACCTGCCCT
AAATGCGGTCATAAAATCAAGGTATGGGAAAATATCCCAGTAATTTCCTGGTTATTATTGCGCGGTAAATGTTCATCATG
CGCCAACCCGATATCCGCTCGCTACCCGATCATCGAAACCATAACCGGTATCTCGACGGTAGTTATCGCTATGCACTTTG
GTGTCAGTTGGCAGGCAGCTGTGATGATGGTATTAAGCTGGTGTCTTATCACCTTAACCATGATTGATTTTGATAAAATG
ATTCTGCCAGATCAAATCACTCTACCATTAGTCTGGATTGGTTTGATTCTTGGTTTAAATAATGTTTTCGTTAGCCTAGA
CCAGGCGGTTATTGGTGCCATCGCCGGTTACATGAGTTTATGGTCTATCTACTGGCTATTTAAAATCGTCACAGGTAAAG
AAGGCATGGGGTATGGCGACTTTAAGCTGTTCGCCGTGTTTGGCGCCTGGTTTGGTTGGCAAGTACTGCCGATGCTTATC
TTGATGGCGTCTTTAGTAGGTGCGATTGTCGGTATTAGTTTAATGGTATTTAAAAGCCATGAAGGCTCAAAGCCTATCCC
GTTTGGTCCTTATCTGGCGGTTGCCGGTTGGATCACCGCATTGTGGGGTGAAGGTATCTGGGCTTGGTATATGGGAATGA
TGGTATAG

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (22-133)

• Type IV leader peptidase family (Peptidase_A24) (143-252)

3D structure

• AlphaFold DB: A0A4P7JRH8

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio campbellii strain DS40M4	52.113	96.271	0.502
pilD	Vibrio cholerae strain A1552	52.113	96.271	0.502
pilD	Acinetobacter baumannii D1279779	47.766	98.644	0.471
pilD	Acinetobacter nosocomialis M2	47.423	98.644	0.468
pilD	Neisseria gonorrhoeae MS11	47.203	96.949	0.458