Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   GNT46_RS27580 Genome accession   NZ_CP046403
Coordinates   5846525..5846989 (-) Length   154 a.a.
NCBI ID   WP_003122079.1    Uniprot ID   P17836
Organism   Pseudomonas aeruginosa strain SE5369     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5841525..5851989
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GNT46_RS27565 (GNT46_27555) nadC 5844035..5844883 (+) 849 WP_003122081.1 carboxylating nicotinate-nucleotide diphosphorylase -
  GNT46_RS27575 (GNT46_27565) - 5845064..5846449 (-) 1386 WP_003122080.1 O-antigen ligase -
  GNT46_RS27580 (GNT46_27570) pilA 5846525..5846989 (-) 465 WP_003122079.1 pilin Machinery gene
  GNT46_RS27585 (GNT46_27575) pilB 5847220..5848920 (+) 1701 WP_003122078.1 type IV-A pilus assembly ATPase PilB Machinery gene
  GNT46_RS27590 (GNT46_27580) pilC 5848924..5850141 (+) 1218 WP_003107299.1 type II secretion system F family protein Machinery gene
  GNT46_RS27595 (GNT46_27585) pilD 5850142..5851014 (+) 873 WP_016852118.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  GNT46_RS27600 (GNT46_27590) coaE 5851011..5851622 (+) 612 WP_003112838.1 dephospho-CoA kinase -
  GNT46_RS27605 (GNT46_27595) yacG 5851619..5851819 (+) 201 WP_003094656.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 154 a.a.        Molecular weight: 16277.67 Da        Isoelectric Point: 8.9987

>NTDB_id=351796 GNT46_RS27580 WP_003122079.1 5846525..5846989(-) (pilA) [Pseudomonas aeruginosa strain SE5369]
MKAQKGFTLIELMIVVAIIGILAAIAIPQYQDYTARTQVTRAVSEVSALKTAAESAILEGKEIVSSATPKDTQYDIGFTE
STLLDGSGKSQIQVTDNKDGTVELVATLGKSSGSAIKGAVITVSRKNDGVWNCKITKTPTAWKPNYAPANCPKS

Nucleotide


Download         Length: 465 bp        

>NTDB_id=351796 GNT46_RS27580 WP_003122079.1 5846525..5846989(-) (pilA) [Pseudomonas aeruginosa strain SE5369]
ATGAAAGCTCAGAAGGGTTTTACTCTGATCGAACTGATGATCGTGGTTGCGATCATCGGCATCCTGGCCGCCATTGCCAT
CCCGCAATACCAGGACTACACCGCCCGTACCCAGGTGACCCGTGCCGTGAGTGAAGTCAGCGCGCTGAAGACCGCTGCGG
AGTCGGCGATTCTGGAAGGGAAGGAGATTGTTTCCAGCGCGACTCCTAAAGATACCCAGTATGACATTGGCTTCACCGAG
TCTACTTTGCTAGATGGTTCTGGTAAGAGTCAGATCCAGGTAACGGACAATAAAGATGGCACCGTTGAGTTGGTCGCTAC
CTTGGGTAAATCTTCTGGTTCCGCCATCAAAGGGGCTGTAATCACTGTTTCGCGTAAAAATGACGGAGTCTGGAACTGCA
AAATCACCAAAACTCCTACAGCTTGGAAGCCCAACTACGCTCCGGCTAATTGCCCGAAATCCTAA

Domains

Predicted by InterProScan.

Pilin (bacterial filament) (Pilin)

(35-151)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  PDB 8V7P

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Acinetobacter baumannii strain A118

44.737

98.701

0.442
  pilA/pilAI Pseudomonas stutzeri DSM 10701

44.444

99.351

0.442
  pilA Pseudomonas aeruginosa PAK

45.985

88.961

0.409
  pilA2 Legionella pneumophila strain ERS1305867

39.073

98.052

0.383
  pilA Vibrio parahaemolyticus RIMD 2210633

37.255

99.351

0.37
  pilA2 Legionella pneumophila str. Paris

38

97.403

0.37
  pilA Vibrio cholerae strain A1552

36.774

100

0.37
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

36.774

100

0.37
  pilA Vibrio cholerae C6706

36.774

100

0.37