Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilG   Type   Machinery gene
Locus tag   EZJ17_RS08295 Genome accession   NZ_CP038018
Coordinates   1606707..1607942 (+) Length   411 a.a.
NCBI ID   WP_067444243.1    Uniprot ID   A0AAE6M538
Organism   Eikenella exigua strain PXX     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1601707..1612942
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EZJ17_RS08285 (EZJ17_08270) recJ 1603060..1604757 (+) 1698 WP_067444245.1 single-stranded-DNA-specific exonuclease RecJ -
  EZJ17_RS08290 (EZJ17_08275) pilF 1605000..1606673 (+) 1674 WP_067441990.1 type IV-A pilus assembly ATPase PilB Machinery gene
  EZJ17_RS08295 (EZJ17_08280) pilG 1606707..1607942 (+) 1236 WP_067444243.1 type II secretion system F family protein Machinery gene
  EZJ17_RS08300 (EZJ17_08285) pilD 1608032..1608889 (+) 858 WP_067444241.1 A24 family peptidase Machinery gene
  EZJ17_RS08305 (EZJ17_08290) coaE 1608891..1609496 (+) 606 WP_067444239.1 dephospho-CoA kinase -
  EZJ17_RS08310 (EZJ17_08295) zapD 1609500..1610276 (+) 777 WP_185737496.1 cell division protein ZapD -
  EZJ17_RS08315 (EZJ17_08300) yacG 1610273..1610455 (+) 183 WP_067442002.1 DNA gyrase inhibitor YacG -
  EZJ17_RS10355 - 1610378..1610599 (-) 222 WP_167508160.1 hypothetical protein -
  EZJ17_RS08320 (EZJ17_08305) rlmD 1610523..1611816 (+) 1294 Protein_1631 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -
  EZJ17_RS08325 (EZJ17_08310) - 1612030..1612824 (+) 795 WP_067444237.1 thymidylate synthase -

Sequence

Protein


Download         Length: 411 a.a.        Molecular weight: 45617.68 Da        Isoelectric Point: 9.4213

>NTDB_id=351367 EZJ17_RS08295 WP_067444243.1 1606707..1607942(+) (pilG) [Eikenella exigua strain PXX]
MVTNKRNNHPKKVAGTSYEFEGRHMESEQIVRGEVVAKDEQDARAKLARRRIKVISLHKKKKVREKKITQSDITVFTRQL
ATMMKAGLPVMQAFDIVAKGHSNASMTKLLMEVRNDIEQGTSMANAFRRHPKYFDDFYCNLVDAGEAGGVLETLLDKLAV
YKEKTQAIKKKIKSALTYPIAVVVVAIVLVIIMMMFVLPEFKKVYDGMNAEMPVLTQFMMGISDFMVAYGWIVIVIMIGS
VVAFVQWYKRSFELQKRIDALLLKMPIFGNIVEKGTIARWARTTATLFTAGVPLVEALESVGGASGNIIYEEATRSIRNQ
VNQGTSLTTAMGSTDLFPNMVLQMASIGEESGSLDDMLNKAAEFYEEEVDIAVSTLSSLMEPIIMVVLGSIIGVILVAMY
LPLFNLGSVVG

Nucleotide


Download         Length: 1236 bp        

>NTDB_id=351367 EZJ17_RS08295 WP_067444243.1 1606707..1607942(+) (pilG) [Eikenella exigua strain PXX]
ATGGTTACAAACAAACGAAACAACCACCCCAAAAAAGTGGCGGGAACCAGCTACGAATTTGAAGGCCGACACATGGAGTC
CGAACAAATCGTCCGTGGCGAAGTAGTTGCCAAAGACGAACAGGATGCCCGTGCCAAACTCGCTCGCCGCCGCATCAAGG
TTATCAGCCTACATAAAAAGAAAAAAGTTCGCGAAAAGAAAATTACCCAGAGCGACATTACCGTTTTTACCCGCCAACTA
GCTACTATGATGAAAGCCGGCCTGCCGGTTATGCAAGCATTTGATATTGTGGCAAAAGGTCACTCCAATGCCTCTATGAC
CAAGCTTTTGATGGAAGTACGCAACGACATCGAGCAAGGGACATCTATGGCCAATGCATTCCGCAGGCATCCTAAATATT
TTGATGATTTCTATTGCAACCTAGTGGATGCCGGTGAGGCAGGTGGTGTACTAGAAACTCTGCTGGACAAACTGGCCGTA
TATAAAGAAAAAACCCAAGCCATCAAAAAGAAAATCAAGAGCGCACTAACTTACCCGATTGCAGTAGTGGTAGTAGCCAT
CGTTTTAGTTATCATCATGATGATGTTCGTATTGCCCGAGTTTAAAAAAGTGTATGACGGTATGAATGCAGAAATGCCGG
TACTTACCCAATTCATGATGGGCATTTCTGATTTCATGGTTGCTTACGGCTGGATCGTAATTGTCATTATGATTGGTTCT
GTAGTAGCTTTCGTTCAATGGTATAAACGCTCTTTTGAGCTGCAAAAACGTATCGATGCCCTCTTGCTCAAAATGCCTAT
CTTCGGCAACATCGTAGAGAAAGGTACAATCGCCCGCTGGGCTCGTACAACCGCCACCCTATTTACCGCTGGTGTACCGC
TGGTGGAGGCACTAGAATCCGTAGGCGGCGCATCAGGTAATATTATTTATGAGGAAGCTACTAGAAGTATCCGTAACCAA
GTAAACCAGGGTACTTCATTGACCACCGCAATGGGCTCAACCGACCTGTTCCCCAACATGGTTCTGCAAATGGCCTCCAT
TGGCGAAGAATCCGGCTCACTGGACGATATGCTGAACAAAGCTGCCGAATTTTATGAAGAGGAAGTAGATATTGCCGTTT
CTACTCTATCGTCCCTGATGGAACCGATTATTATGGTGGTATTAGGCAGCATTATTGGTGTGATTCTAGTTGCCATGTAC
CTGCCACTGTTCAACCTGGGCAGCGTAGTTGGCTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(76-199)

Type II secretion system (T2SS), protein F (T2SSF)

(280-402)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilG Neisseria gonorrhoeae MS11

65.217

95.134

0.62
  pilG Neisseria meningitidis 44/76-A

64.706

95.134

0.616
  pilC Legionella pneumophila strain ERS1305867

48.995

96.837

0.474
  pilC Pseudomonas stutzeri DSM 10701

45.161

98.054

0.443
  pilC Acinetobacter baumannii D1279779

45.363

97.08

0.44
  pilC Acinetobacter baylyi ADP1

45.337

93.917

0.426
  pilC Vibrio cholerae strain A1552

38.095

97.08

0.37
  pilC Vibrio campbellii strain DS40M4

37.594

97.08

0.365

Multiple sequence alignment