Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   EZJ17_RS08290 Genome accession   NZ_CP038018
Coordinates   1605000..1606673 (+) Length   557 a.a.
NCBI ID   WP_067441990.1    Uniprot ID   A0AAX1F929
Organism   Eikenella exigua strain PXX     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1600000..1611673
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EZJ17_RS08270 (EZJ17_08255) fumC 1600931..1602325 (-) 1395 WP_067444247.1 class II fumarate hydratase -
  EZJ17_RS08285 (EZJ17_08270) recJ 1603060..1604757 (+) 1698 WP_067444245.1 single-stranded-DNA-specific exonuclease RecJ -
  EZJ17_RS08290 (EZJ17_08275) pilF 1605000..1606673 (+) 1674 WP_067441990.1 type IV-A pilus assembly ATPase PilB Machinery gene
  EZJ17_RS08295 (EZJ17_08280) pilG 1606707..1607942 (+) 1236 WP_067444243.1 type II secretion system F family protein Machinery gene
  EZJ17_RS08300 (EZJ17_08285) pilD 1608032..1608889 (+) 858 WP_067444241.1 A24 family peptidase Machinery gene
  EZJ17_RS08305 (EZJ17_08290) coaE 1608891..1609496 (+) 606 WP_067444239.1 dephospho-CoA kinase -
  EZJ17_RS08310 (EZJ17_08295) zapD 1609500..1610276 (+) 777 WP_185737496.1 cell division protein ZapD -
  EZJ17_RS08315 (EZJ17_08300) yacG 1610273..1610455 (+) 183 WP_067442002.1 DNA gyrase inhibitor YacG -
  EZJ17_RS10355 - 1610378..1610599 (-) 222 WP_167508160.1 hypothetical protein -

Sequence

Protein


Download         Length: 557 a.a.        Molecular weight: 62106.78 Da        Isoelectric Point: 5.1922

>NTDB_id=351366 EZJ17_RS08290 WP_067441990.1 1605000..1606673(+) (pilF) [Eikenella exigua strain PXX]
MSVGLLRILFQTKLIEQDQVEKYREKVNQNKNIIPILAEAGIIKQTDLAQLLAKLFHYPILDLSLYSRSMMVQDVLNDSQ
MMEYRCVPLFKRGQKLFLGVSDPTLLQTYQKLVFPSGLIVDLILVNDEQLDRLLEFASQTSTAILDEISADPTGVTAPAL
KVDDEEEDGPIAKFIHKVLSDALNTGASDIHFEFYEEMARIRFRTDGQLREVVQPPLNIRGQIASRIKVMSKMDISEKRV
PQDGRIQIQFNKNQKPIDFRVNTLPTLFGEKIVMRILNSDAATLNINQLGFEPFQKELLLEAIHRPYGMVLVTGPTGSGK
TVSLYTCLNILNTDSVNISTAEDPAEINLPGINQVNVNDKQGLTFAAALKAFLRQDPDIIMVGEIRDLETADIAIKAAQT
GHMVFSTLHTNNAPATLSRMLNMGVAPFNIASSVNLIMAQRLVRRLCTCKQPIERPPAEALLKVGFTEEDLTKDDWQLYR
QVGCDRCKGKGFKGRAGVYEIMPVTDAMQKIIINNGTEVDIAEQAYADGLVDLRRAGILKAMQGITTLEEILASTND

Nucleotide


Download         Length: 1674 bp        

>NTDB_id=351366 EZJ17_RS08290 WP_067441990.1 1605000..1606673(+) (pilF) [Eikenella exigua strain PXX]
ATGAGCGTCGGACTATTGCGCATCCTATTTCAAACCAAGCTGATTGAACAGGATCAGGTTGAAAAATACCGAGAAAAGGT
TAATCAAAACAAAAATATAATTCCAATTCTGGCCGAAGCCGGCATCATCAAGCAGACAGATTTGGCACAACTTTTGGCCA
AGTTGTTCCACTATCCGATTTTAGATTTGTCCCTCTACTCCCGCAGCATGATGGTGCAGGATGTGCTCAACGACAGCCAG
ATGATGGAGTACCGCTGTGTACCTTTGTTCAAACGCGGACAAAAACTGTTTTTGGGTGTCTCCGATCCCACTTTGTTGCA
AACATACCAAAAGCTCGTTTTCCCTAGCGGCCTAATTGTCGACCTGATTCTGGTAAACGACGAGCAACTAGATCGTCTGC
TGGAATTCGCCAGCCAAACATCCACAGCCATTTTGGATGAAATTTCTGCCGATCCCACAGGCGTGACCGCACCGGCACTC
AAGGTGGATGACGAAGAAGAAGACGGCCCGATTGCCAAATTCATCCACAAAGTGCTGTCTGATGCCCTCAACACTGGCGC
ATCTGATATTCACTTTGAGTTCTACGAAGAAATGGCACGCATCCGCTTCCGTACTGACGGTCAGCTGCGTGAAGTGGTAC
AACCACCACTGAATATCCGCGGGCAGATTGCTTCACGTATAAAAGTAATGTCCAAAATGGATATTTCTGAAAAACGCGTT
CCACAAGACGGCCGTATTCAGATTCAGTTCAATAAAAACCAAAAGCCCATCGACTTCCGTGTGAATACGCTGCCCACCCT
ATTCGGCGAAAAAATCGTAATGCGTATTTTGAATTCGGATGCTGCCACGCTGAACATCAACCAACTGGGTTTCGAACCTT
TCCAAAAAGAATTGCTGCTGGAAGCCATCCACCGCCCCTATGGAATGGTACTGGTTACTGGCCCGACCGGTTCCGGTAAA
ACCGTGTCACTGTATACCTGCTTGAACATTTTAAATACCGACAGTGTAAACATTTCCACTGCCGAAGATCCGGCCGAGAT
TAACCTGCCTGGCATCAATCAAGTAAATGTGAACGATAAACAAGGCTTGACATTTGCCGCAGCACTGAAGGCTTTCCTGC
GCCAGGATCCAGACATCATCATGGTAGGTGAGATTCGTGATTTGGAAACCGCCGATATTGCCATTAAAGCGGCACAAACC
GGCCACATGGTGTTCTCCACCCTGCACACCAACAACGCCCCGGCCACTCTATCCCGTATGCTGAACATGGGGGTAGCACC
GTTCAATATCGCCAGCTCGGTTAATCTGATTATGGCACAGCGTTTGGTGCGCCGACTCTGCACCTGCAAGCAACCAATCG
AACGTCCGCCTGCCGAGGCTTTACTGAAAGTCGGCTTCACTGAAGAAGATTTGACCAAGGATGACTGGCAGCTATACCGT
CAAGTGGGTTGCGACCGTTGCAAAGGCAAAGGCTTCAAAGGCCGTGCCGGTGTATATGAAATCATGCCGGTTACTGATGC
CATGCAGAAAATCATCATCAATAACGGCACCGAGGTGGATATTGCCGAACAAGCCTATGCCGACGGACTGGTGGACTTGC
GCCGAGCCGGTATTTTAAAAGCAATGCAAGGTATTACCACCTTAGAAGAAATTCTTGCCAGCACCAATGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(177-445)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(37-135)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

69.946

100

0.702
  pilB Acinetobacter baylyi ADP1

51.706

100

0.517
  pilB Acinetobacter baumannii D1279779

50.899

99.82

0.508
  pilB Vibrio cholerae strain A1552

46.479

100

0.474
  pilB Legionella pneumophila strain ERS1305867

45.989

100

0.463
  pilB Vibrio parahaemolyticus RIMD 2210633

44.405

100

0.449
  pilB Vibrio campbellii strain DS40M4

43.134

100

0.44
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.19

94.255

0.379
  pilF Thermus thermophilus HB27

37.276

100

0.373

Multiple sequence alignment