Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   E2I05_RS20170 Genome accession   NZ_CP037952
Coordinates   5096689..5097957 (+) Length   422 a.a.
NCBI ID   WP_121854477.1    Uniprot ID   A0A3A6TQA0
Organism   Parashewanella spongiae strain HJ039     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5091689..5102957
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E2I05_RS20155 - 5091798..5093255 (-) 1458 WP_121854474.1 hypothetical protein -
  E2I05_RS22810 - 5094160..5094609 (+) 450 WP_121854475.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  E2I05_RS20165 pilB 5094770..5096479 (+) 1710 WP_121854476.1 type IV-A pilus assembly ATPase PilB Machinery gene
  E2I05_RS20170 pilC 5096689..5097957 (+) 1269 WP_121854477.1 type II secretion system F family protein Machinery gene
  E2I05_RS20175 - 5098081..5100144 (+) 2064 WP_121854478.1 hypothetical protein -
  E2I05_RS20180 pilD 5100238..5101155 (+) 918 WP_121854479.1 A24 family peptidase Machinery gene
  E2I05_RS20185 coaE 5101186..5101812 (+) 627 WP_121854480.1 dephospho-CoA kinase -
  E2I05_RS20190 zapD 5101815..5102549 (+) 735 WP_121854481.1 cell division protein ZapD -
  E2I05_RS20195 yacG 5102593..5102808 (+) 216 WP_121854482.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 422 a.a.        Molecular weight: 46410.77 Da        Isoelectric Point: 10.1559

>NTDB_id=350926 E2I05_RS20170 WP_121854477.1 5096689..5097957(+) (pilC) [Parashewanella spongiae strain HJ039]
MAIANPASHTSRRKPKKPKAEPKVVTFQWKGTNRDGKKTSGELQAISMTEARVMLRSQGISPKSVRKKAASLFSGDKPIK
PMDIAMITRQIATMLSAGVPLVTTIQMLGQGHEKPKVRQLLGTILNDLEAGIPLSDALRPHRRYFDELYVDLVAAGENSG
ALDTVFDRIATYREKSEALKSKIKKAMFYPIAVTVVAILVTVGMLLFVVPQFEDIFKSFGAELPAFTQFILMLSRGLQEW
WWVFFIAIVAGVFLFIRSHRNSQKFRDQVDGIKLKIPVIGEILHKAAMARFARTLATTFAAGVPLIEGIESAAGASGNAV
YRDALLKVRQEVMSGMQMNVAMRTTDLFPDMLIQMVMIGEESGSLDNMLNKVANIYEMQVDDAVDGLASLIEPIMMVVIG
TIVGGLIVGMYLPIFEMGKVVG

Nucleotide


Download         Length: 1269 bp        

>NTDB_id=350926 E2I05_RS20170 WP_121854477.1 5096689..5097957(+) (pilC) [Parashewanella spongiae strain HJ039]
ATGGCCATTGCCAACCCAGCAAGTCACACAAGCCGTCGTAAGCCCAAAAAACCTAAGGCCGAGCCTAAGGTCGTCACCTT
TCAATGGAAAGGTACCAACCGCGATGGCAAAAAAACCTCCGGTGAACTGCAAGCAATCTCAATGACTGAAGCGCGAGTCA
TGTTGCGCAGTCAGGGCATTAGTCCTAAATCAGTGCGTAAAAAAGCCGCCTCCTTGTTCAGTGGTGATAAGCCAATCAAA
CCTATGGACATCGCCATGATCACCCGCCAGATAGCCACTATGCTATCAGCCGGAGTGCCATTGGTAACCACTATTCAAAT
GCTCGGCCAAGGCCACGAAAAACCAAAAGTCCGCCAACTGCTCGGCACCATATTGAATGATTTAGAGGCGGGGATCCCGC
TGTCAGACGCTTTGCGCCCACACCGCCGTTACTTTGATGAACTCTATGTTGATTTAGTCGCCGCTGGTGAAAATTCAGGG
GCGCTAGATACGGTTTTTGACCGCATTGCCACTTACCGTGAAAAGTCCGAAGCCTTAAAATCCAAAATAAAAAAAGCCAT
GTTTTACCCGATTGCCGTCACCGTTGTCGCCATTTTAGTAACGGTTGGCATGTTGCTGTTTGTTGTACCGCAATTTGAAG
ATATCTTTAAAAGCTTTGGTGCCGAGTTGCCTGCTTTTACCCAATTTATTTTAATGTTATCTCGCGGGTTACAAGAGTGG
TGGTGGGTTTTCTTCATTGCCATTGTCGCTGGAGTGTTTTTATTTATCCGCAGCCATCGTAATTCCCAAAAGTTTCGCGA
CCAAGTGGATGGAATAAAGCTCAAAATTCCTGTTATTGGTGAAATACTGCATAAAGCCGCTATGGCACGTTTTGCCCGCA
CCTTAGCCACCACTTTTGCCGCTGGTGTACCACTGATTGAAGGCATAGAGTCAGCCGCTGGTGCTTCTGGCAATGCGGTA
TATCGTGATGCCTTGCTGAAAGTACGTCAAGAAGTCATGTCTGGCATGCAAATGAATGTGGCCATGCGCACCACCGATTT
GTTCCCCGATATGCTCATTCAAATGGTGATGATTGGTGAAGAATCCGGCTCGCTGGATAATATGCTTAACAAAGTGGCTA
ATATTTATGAAATGCAAGTAGACGACGCCGTCGATGGCTTAGCCAGCTTGATTGAACCCATTATGATGGTGGTCATTGGT
ACTATTGTTGGCGGCTTAATTGTGGGCATGTACTTACCCATATTCGAAATGGGTAAAGTGGTGGGGTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(88-210)

Type II secretion system (T2SS), protein F (T2SSF)

(291-413)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3A6TQA0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

54.177

93.602

0.507
  pilC Acinetobacter baylyi ADP1

49.755

96.682

0.481
  pilC Legionella pneumophila strain ERS1305867

49.752

95.735

0.476
  pilC Acinetobacter baumannii D1279779

50

94.313

0.472
  pilC Vibrio cholerae strain A1552

47.5

94.787

0.45
  pilC Vibrio campbellii strain DS40M4

43.781

95.261

0.417
  pilG Neisseria meningitidis 44/76-A

40.447

95.498

0.386
  pilG Neisseria gonorrhoeae MS11

40.447

95.498

0.386

Multiple sequence alignment