Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   E2I05_RS20180 Genome accession   NZ_CP037952
Coordinates   5100238..5101155 (+) Length   305 a.a.
NCBI ID   WP_121854479.1    Uniprot ID   A0A3A6TGF3
Organism   Parashewanella spongiae strain HJ039     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5095238..5106155
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E2I05_RS20170 pilC 5096689..5097957 (+) 1269 WP_121854477.1 type II secretion system F family protein Machinery gene
  E2I05_RS20175 - 5098081..5100144 (+) 2064 WP_121854478.1 hypothetical protein -
  E2I05_RS20180 pilD 5100238..5101155 (+) 918 WP_121854479.1 A24 family peptidase Machinery gene
  E2I05_RS20185 coaE 5101186..5101812 (+) 627 WP_121854480.1 dephospho-CoA kinase -
  E2I05_RS20190 zapD 5101815..5102549 (+) 735 WP_121854481.1 cell division protein ZapD -
  E2I05_RS20195 yacG 5102593..5102808 (+) 216 WP_121854482.1 DNA gyrase inhibitor YacG -
  E2I05_RS20200 mutT 5102805..5103200 (+) 396 WP_121854483.1 8-oxo-dGTP diphosphatase MutT -
  E2I05_RS20205 - 5103215..5103487 (-) 273 WP_121854484.1 HU family DNA-binding protein -

Sequence

Protein


Download         Length: 305 a.a.        Molecular weight: 33700.08 Da        Isoelectric Point: 7.0178

>NTDB_id=350927 E2I05_RS20180 WP_121854479.1 5100238..5101155(+) (pilD) [Parashewanella spongiae strain HJ039]
MSEFLILLNAQPYLFVVIAFIFAAMLGSFINVVIHRLPVMLKREWQQECNQYLNEYHPEKVKKLGETELNKAIDPFPEKY
NLIVPTSACPKCNSKIKPWHNLPILGWLILKGKCANCGNAISARYPIIEFISGALVATLAWHFGPTIDFVATAVLTLVLI
ALTGIDLDEMLLPDNLTLPLLWLGIIFSLTDGFVSLNDSVIGAVAGYLSLWSVFWLFKIVTGKEGMGYGDFKLMAVFGAW
LGWQMLPLIILLSSLVGAVVGISLIVSKKLHSGNPIPFGPYIAAAGWIAVVWGQDIVNWYLAHAI

Nucleotide


Download         Length: 918 bp        

>NTDB_id=350927 E2I05_RS20180 WP_121854479.1 5100238..5101155(+) (pilD) [Parashewanella spongiae strain HJ039]
ATGTCTGAGTTTCTCATTTTACTGAACGCACAACCATACTTGTTTGTCGTCATCGCTTTTATTTTTGCTGCGATGCTCGG
CAGTTTCATTAACGTCGTAATCCATCGACTCCCTGTGATGTTAAAAAGAGAATGGCAACAAGAGTGTAACCAGTACCTCA
ATGAATATCATCCAGAAAAAGTCAAAAAATTAGGTGAAACTGAACTCAACAAAGCCATTGATCCCTTTCCTGAGAAATAC
AATTTAATCGTGCCGACTTCGGCGTGCCCTAAATGCAACAGCAAGATCAAACCTTGGCACAATTTACCCATTTTAGGTTG
GTTGATACTCAAAGGAAAATGCGCAAACTGTGGCAATGCTATTTCTGCTCGTTACCCGATTATTGAGTTCATCAGTGGCG
CATTAGTGGCAACATTAGCGTGGCATTTCGGGCCAACCATAGATTTTGTAGCAACTGCAGTATTAACCCTAGTGTTAATT
GCACTTACAGGTATTGATCTTGATGAAATGTTACTCCCTGATAATTTAACCTTACCGCTATTGTGGCTGGGAATAATTTT
TAGCTTAACCGATGGTTTTGTGAGCCTAAATGACTCAGTCATTGGTGCCGTTGCAGGGTATTTGAGTTTATGGTCTGTAT
TCTGGTTATTTAAGATCGTCACAGGTAAGGAAGGCATGGGTTATGGCGACTTCAAATTAATGGCCGTATTTGGCGCGTGG
CTTGGCTGGCAAATGTTGCCCTTAATTATTTTACTGTCCTCTCTTGTTGGCGCAGTTGTTGGTATTAGTCTGATTGTGAG
CAAAAAACTTCATTCAGGTAACCCAATTCCATTTGGCCCTTATATCGCCGCTGCAGGATGGATAGCTGTGGTTTGGGGTC
AAGACATTGTGAATTGGTACTTAGCTCATGCAATTTAA

Domains

Predicted by InterproScan.

Type IV leader peptidase family (Peptidase_A24)

(154-262)

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(21-143)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3A6TGF3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio campbellii strain DS40M4

51.027

95.738

0.489
  pilD Vibrio cholerae strain A1552

49.488

96.066

0.475
  pilD Acinetobacter nosocomialis M2

46.713

94.754

0.443
  pilD Acinetobacter baumannii D1279779

46.367

94.754

0.439
  pilD Neisseria gonorrhoeae MS11

43.345

96.066

0.416

Multiple sequence alignment