Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   DAIF1_RS16705 Genome accession   NZ_CP037883
Coordinates   3643281..3644540 (+) Length   419 a.a.
NCBI ID   WP_080150567.1    Uniprot ID   A0A1W1H3H8
Organism   Stenotrophomonas indicatrix strain DAIF1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3638281..3649540
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DAIF1_RS16685 (DAIF1_33030) pilR 3638392..3639765 (+) 1374 WP_025874296.1 sigma-54 dependent transcriptional regulator Regulator
  DAIF1_RS16690 (DAIF1_33040) pilB 3640003..3641730 (-) 1728 WP_059064907.1 type IV-A pilus assembly ATPase PilB Machinery gene
  DAIF1_RS16695 (DAIF1_33050) - 3641950..3642357 (-) 408 WP_080150564.1 pilin -
  DAIF1_RS16700 (DAIF1_33060) - 3642507..3642929 (-) 423 WP_080150566.1 pilin -
  DAIF1_RS16705 (DAIF1_33070) pilC 3643281..3644540 (+) 1260 WP_080150567.1 type II secretion system F family protein Machinery gene
  DAIF1_RS16710 (DAIF1_33080) - 3644548..3645411 (+) 864 WP_025874291.1 A24 family peptidase -
  DAIF1_RS16715 (DAIF1_33090) coaE 3645423..3646034 (+) 612 WP_134833003.1 dephospho-CoA kinase -
  DAIF1_RS16720 (DAIF1_33100) - 3646087..3646473 (-) 387 WP_134833004.1 hypothetical protein -
  DAIF1_RS16725 (DAIF1_33110) - 3646986..3648356 (-) 1371 WP_080150572.1 HAMP domain-containing sensor histidine kinase -
  DAIF1_RS16730 (DAIF1_33120) - 3648319..3648996 (-) 678 WP_025874286.1 response regulator transcription factor -
  DAIF1_RS16735 (DAIF1_33130) - 3649005..3649478 (-) 474 WP_134833005.1 hypothetical protein -

Sequence

Protein


Download         Length: 419 a.a.        Molecular weight: 45904.37 Da        Isoelectric Point: 10.1703

>NTDB_id=350436 DAIF1_RS16705 WP_080150567.1 3643281..3644540(+) (pilC) [Stenotrophomonas indicatrix strain DAIF1]
MSVSRSAIKKEPVARATMELQPFVWEGTDKRGVKMKGEQLAKNANLLRAELRRQGINPGTVKTKPKPLFGAAGKPIKPRD
IAFFSRQMATMMKSGVPIVSALEIIGSGHKNPRMKKMVDTIRTDIEGGSSMYEAVSKHPVQFDELYRNLVRAGEGAGVLE
TVLDTVATYKENIEALKGKIKKALFYPIMVVVVAMLVSGIMLVFVVPQFEDVFKSFGADLPAFTQMVVNLSRFMVSWWWL
MLIVAIGTAVGAVMAYKRSPKMQHAMDRFVLKVPVIGQIMHNSAIARFARTTAVTFKAGVPLVEALSIVAGATGNTVYEE
AVLRMRDDVSVGYPVNVSMKQTNLFPHMVIQMTGIGEEAGALDAMLFKVAEYYEQEVNNSVDALSSLLEPMIMVFIGTIV
GGMVIAMYLPIFKLGAVVG

Nucleotide


Download         Length: 1260 bp        

>NTDB_id=350436 DAIF1_RS16705 WP_080150567.1 3643281..3644540(+) (pilC) [Stenotrophomonas indicatrix strain DAIF1]
ATGTCTGTCAGTCGCAGTGCGATCAAGAAAGAACCGGTGGCGCGCGCCACCATGGAGCTGCAGCCGTTTGTCTGGGAGGG
GACCGACAAGCGGGGCGTGAAGATGAAGGGCGAGCAGTTGGCCAAGAATGCCAACCTGCTGAGGGCCGAACTCCGTCGCC
AGGGCATCAACCCCGGTACGGTGAAGACGAAGCCGAAGCCGTTGTTCGGTGCTGCCGGAAAACCGATCAAACCGCGGGAC
ATCGCCTTCTTCAGCCGGCAGATGGCCACGATGATGAAGTCCGGCGTACCGATCGTGTCGGCACTGGAGATCATCGGCAG
CGGGCACAAGAACCCGCGCATGAAGAAGATGGTGGACACCATCCGCACCGATATCGAGGGCGGCTCCTCGATGTACGAAG
CGGTCAGCAAGCATCCGGTGCAGTTCGACGAGCTCTATCGCAACCTAGTGCGGGCCGGCGAAGGCGCCGGCGTGCTGGAA
ACCGTGCTGGACACGGTGGCCACCTACAAAGAGAACATCGAGGCGCTGAAGGGCAAGATCAAGAAGGCGCTGTTCTACCC
GATCATGGTGGTGGTGGTGGCCATGCTGGTCAGCGGCATCATGTTGGTATTTGTGGTGCCGCAGTTCGAGGATGTGTTCA
AGAGTTTCGGCGCCGACCTGCCGGCGTTTACCCAGATGGTGGTGAACCTGTCGCGGTTCATGGTGTCGTGGTGGTGGCTG
ATGCTGATTGTTGCGATCGGAACGGCGGTCGGCGCGGTCATGGCCTACAAGCGATCGCCAAAGATGCAGCACGCCATGGA
CCGGTTCGTGTTGAAGGTGCCGGTGATCGGCCAGATCATGCACAACAGCGCCATTGCCCGTTTCGCCCGCACCACCGCCG
TTACGTTCAAGGCGGGCGTGCCGCTGGTGGAGGCACTCAGCATCGTGGCGGGCGCCACCGGCAATACGGTGTATGAGGAG
GCGGTGCTGCGGATGCGCGATGACGTGTCTGTCGGCTACCCGGTCAACGTTTCCATGAAACAGACCAACCTGTTCCCGCA
CATGGTCATCCAGATGACCGGCATCGGCGAAGAGGCCGGCGCCCTGGATGCCATGCTGTTCAAGGTGGCCGAGTACTACG
AGCAGGAAGTCAACAATTCGGTGGATGCCCTGAGCAGCCTGCTGGAACCGATGATCATGGTGTTCATTGGTACCATTGTT
GGCGGCATGGTGATTGCCATGTACCTTCCCATCTTCAAGCTCGGCGCCGTCGTCGGATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(84-207)

Type II secretion system (T2SS), protein F (T2SSF)

(288-410)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1W1H3H8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

55.051

94.511

0.52
  pilC Acinetobacter baylyi ADP1

51.597

97.136

0.501
  pilC Legionella pneumophila strain ERS1305867

52.381

95.227

0.499
  pilC Acinetobacter baumannii D1279779

50.63

94.749

0.48
  pilG Neisseria gonorrhoeae MS11

44.888

95.704

0.43
  pilG Neisseria meningitidis 44/76-A

44.638

95.704

0.427
  pilC Vibrio cholerae strain A1552

41.133

96.897

0.399
  pilC Vibrio campbellii strain DS40M4

40.648

95.704

0.389

Multiple sequence alignment