Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   DAIF1_RS16690 Genome accession   NZ_CP037883
Coordinates   3640003..3641730 (-) Length   575 a.a.
NCBI ID   WP_059064907.1    Uniprot ID   -
Organism   Stenotrophomonas indicatrix strain DAIF1     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3635003..3646730
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DAIF1_RS16675 (DAIF1_33010) sucC 3635178..3636347 (-) 1170 WP_025874298.1 ADP-forming succinate--CoA ligase subunit beta -
  DAIF1_RS16680 (DAIF1_33020) - 3636724..3638337 (+) 1614 WP_134833002.1 ATP-binding protein -
  DAIF1_RS16685 (DAIF1_33030) pilR 3638392..3639765 (+) 1374 WP_025874296.1 sigma-54 dependent transcriptional regulator Regulator
  DAIF1_RS16690 (DAIF1_33040) pilB 3640003..3641730 (-) 1728 WP_059064907.1 type IV-A pilus assembly ATPase PilB Machinery gene
  DAIF1_RS16695 (DAIF1_33050) - 3641950..3642357 (-) 408 WP_080150564.1 pilin -
  DAIF1_RS16700 (DAIF1_33060) - 3642507..3642929 (-) 423 WP_080150566.1 pilin -
  DAIF1_RS16705 (DAIF1_33070) pilC 3643281..3644540 (+) 1260 WP_080150567.1 type II secretion system F family protein Machinery gene
  DAIF1_RS16710 (DAIF1_33080) - 3644548..3645411 (+) 864 WP_025874291.1 A24 family peptidase -
  DAIF1_RS16715 (DAIF1_33090) coaE 3645423..3646034 (+) 612 WP_134833003.1 dephospho-CoA kinase -
  DAIF1_RS16720 (DAIF1_33100) - 3646087..3646473 (-) 387 WP_134833004.1 hypothetical protein -

Sequence

Protein


Download         Length: 575 a.a.        Molecular weight: 62306.25 Da        Isoelectric Point: 5.8722

>NTDB_id=350435 DAIF1_RS16690 WP_059064907.1 3640003..3641730(-) (pilB) [Stenotrophomonas indicatrix strain DAIF1]
MNAVTTANLVGITGLARRLVQDGALDEVAARDAMAKAAAARQPLPTWFAQNKLVSASQLAAANAVEFGMPLFDVSTFDSS
QNAMNLVSEELLRKHNVLPLFKRGGKLFVGTSNPTHSLDEIKFHTNLVVEPILVDEDQIRRTLEQWHASHDTIGDALGGD
DDGMANLDVGGGDDDMASTDSGIDAKGDDTPVVKFINKVLIDAIRKGASDIHFEPYEDDYRVRLRIDGLLKMVARAPNKL
NQRIAARLKVMAQLDIAEKRVPQDGRIKLNLSKTRQIDFRVSTLPTLFGEKVVLRILDGSAAKLGIDKLGYEPDQQKLFL
EAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAAALRSFLRQDPDII
MVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCSNCKRPANLPEHA
LLAEGFTQAQLDAGIELYEAVGCDECTEGYKGRTGLYQVMPMTDEIATIVLAGGNALQIAEAAQAIGVNDLRQSALKKAA
AGVTSLAEINRVTKD

Nucleotide


Download         Length: 1728 bp        

>NTDB_id=350435 DAIF1_RS16690 WP_059064907.1 3640003..3641730(-) (pilB) [Stenotrophomonas indicatrix strain DAIF1]
ATGAACGCCGTTACCACCGCCAATCTCGTCGGCATCACCGGCCTGGCCCGCCGCCTCGTCCAGGACGGCGCGCTGGACGA
AGTCGCTGCCCGCGATGCCATGGCCAAGGCGGCCGCCGCTCGCCAGCCCCTGCCGACGTGGTTCGCGCAGAACAAGCTGG
TCAGCGCCTCGCAGCTCGCTGCCGCCAACGCCGTCGAATTCGGCATGCCGCTGTTCGACGTGTCCACGTTCGACAGCAGC
CAGAATGCGATGAACCTGGTCAGCGAAGAGCTGCTGCGCAAGCACAACGTGCTGCCGCTGTTCAAACGCGGCGGCAAGTT
GTTCGTCGGCACCAGCAACCCCACCCACTCGCTGGACGAGATCAAGTTCCACACCAATCTGGTGGTTGAGCCGATCCTGG
TGGACGAGGACCAGATCCGCCGCACGTTGGAGCAGTGGCATGCCAGCCATGACACGATCGGCGATGCGCTGGGTGGCGAC
GATGACGGCATGGCCAACCTCGATGTTGGCGGTGGCGACGACGACATGGCCAGCACCGACAGCGGTATCGACGCCAAGGG
CGACGACACCCCGGTGGTCAAGTTCATCAACAAGGTGCTGATTGACGCGATCCGCAAGGGCGCGTCGGACATCCATTTCG
AGCCCTACGAAGACGACTACCGCGTGCGTCTGCGCATCGACGGCCTGCTGAAGATGGTCGCGCGCGCGCCGAACAAGCTC
AACCAGCGCATCGCCGCGCGCCTGAAGGTGATGGCGCAGCTGGATATCGCCGAAAAGCGCGTGCCGCAGGACGGGCGCAT
CAAGCTCAACCTGTCCAAGACCCGGCAGATCGACTTCCGCGTCAGCACGCTGCCTACGCTGTTCGGTGAGAAGGTGGTGC
TGCGTATCCTCGATGGCAGCGCGGCCAAGCTGGGCATCGACAAGCTGGGTTACGAGCCGGACCAGCAGAAGCTGTTCCTG
GAGGCAATCCACAAGCCGTACGGCATGGTGCTGGTGACCGGCCCGACCGGTTCGGGCAAGACGGTGTCGCTGTATACCGC
GCTGGGCATCCTCAACGACGAGACCCGCAACATTTCCACCGCGGAAGATCCGGTGGAAATCCGCCTGCCCGGCGTCAACC
AGGTGCAGCAGAACAACAAGCGTGGCATGACCTTTGCCGCGGCGCTGCGTTCGTTCCTGCGACAGGATCCGGACATCATC
ATGGTGGGCGAAATCCGCGATCTGGAAACGGCGGAAATTGCGATCAAGGCTGCGCAGACCGGTCACATGGTGCTGTCCAC
GCTGCATACCAACGATGCGCCGCAGACCATCGCGCGCCTGATGAACATGGGCATCGCGCCGTACAACATCACCAGCTCGG
TGACGCTGGTGATTGCCCAGCGCCTGGCGCGCCGCCTGTGTTCGAACTGCAAGCGCCCGGCCAATCTGCCGGAGCATGCG
CTGCTGGCCGAGGGCTTCACCCAGGCGCAGCTGGATGCAGGCATCGAACTGTATGAGGCAGTGGGCTGCGACGAATGCAC
CGAGGGCTACAAGGGCCGTACCGGCCTCTACCAGGTGATGCCGATGACCGACGAGATCGCGACCATCGTGCTGGCGGGCG
GCAACGCGCTGCAGATTGCCGAGGCCGCGCAGGCGATCGGCGTGAATGACCTGCGGCAGTCGGCGTTGAAGAAGGCGGCC
GCGGGCGTGACCAGCCTGGCCGAGATCAATCGCGTCACCAAGGATTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(198-465)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(49-149)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

55.575

100

0.563
  pilB Acinetobacter baumannii D1279779

56.437

98.609

0.557
  pilB Legionella pneumophila strain ERS1305867

52.113

98.783

0.515
  pilF Neisseria gonorrhoeae MS11

49.296

98.783

0.487
  pilB Vibrio cholerae strain A1552

49.037

99.304

0.487
  pilB Vibrio parahaemolyticus RIMD 2210633

45.884

99.304

0.456
  pilB Vibrio campbellii strain DS40M4

44.58

99.478

0.443

Multiple sequence alignment