Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   EZZ25_RS06355 Genome accession   NZ_CP036499
Coordinates   1298208..1299896 (-) Length   562 a.a.
NCBI ID   WP_000957192.1    Uniprot ID   -
Organism   Vibrio cholerae strain 20000     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1293208..1304896
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EZZ25_RS06325 (EZZ25_06325) yacG 1294274..1294471 (-) 198 WP_000162865.1 DNA gyrase inhibitor YacG -
  EZZ25_RS06335 (EZZ25_06335) zapD 1294629..1295369 (-) 741 WP_000207194.1 cell division protein ZapD -
  EZZ25_RS06340 (EZZ25_06340) coaE 1295401..1296009 (-) 609 WP_000011556.1 dephospho-CoA kinase -
  EZZ25_RS06345 (EZZ25_06345) pilD 1296006..1296881 (-) 876 WP_000418751.1 A24 family peptidase Machinery gene
  EZZ25_RS06350 (EZZ25_06350) pilC 1296939..1298165 (-) 1227 WP_000648513.1 type II secretion system F family protein Machinery gene
  EZZ25_RS06355 (EZZ25_06355) pilB 1298208..1299896 (-) 1689 WP_000957192.1 type IV-A pilus assembly ATPase PilB Machinery gene
  EZZ25_RS06360 (EZZ25_06360) - 1299896..1300381 (-) 486 WP_000649332.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  EZZ25_RS06365 (EZZ25_06365) nadC 1300626..1301516 (-) 891 WP_000665278.1 carboxylating nicotinate-nucleotide diphosphorylase -
  EZZ25_RS06370 (EZZ25_06370) ampD 1301814..1302359 (+) 546 WP_000567319.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  EZZ25_RS06375 (EZZ25_06375) fldB 1302510..1303028 (-) 519 WP_000690112.1 flavodoxin FldB -
  EZZ25_RS06380 (EZZ25_06380) xerD 1303166..1304074 (+) 909 WP_001287472.1 site-specific tyrosine recombinase XerD -
  EZZ25_RS06385 (EZZ25_06385) dsbC 1304091..1304843 (+) 753 WP_000115049.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -

Sequence

Protein


Download         Length: 562 a.a.        Molecular weight: 62197.32 Da        Isoelectric Point: 6.2600

>NTDB_id=349232 EZZ25_RS06355 WP_000957192.1 1298208..1299896(-) (pilB) [Vibrio cholerae strain 20000]
MLTNLVAILRQAELISAMQEQAVVTQVSASGTSVPEALLGLGFFHPRELTEQLSHIFGLQETDLSRYDYANLCQQLGLRE
LITRYDALPIAKQGNLLLLAVSDPTLLQAEEEFRFATGLQVELALADHRALQAAIRRLYGRSIQGAANQGKEISQDELAN
LVKVSDDELQSIEDLSQDDSPVSRFINQVLLDAVRKGASDIHFEPYENQYRIRLRCDGILVETQQPASHLSRRLAARIKI
LSKLDIAERRLPQDGRIKLRLSRDTAIDMRVSTLPTLWGEKIVLRLLDSSAANLDIDKLGYNPQQKQLYLNALKRPQGMI
LMTGPTGSGKTVSLYTGLRILNTSQINISTAEDPVEINLSGINQVQVQPKIGFGFAEALRSFLRQDPDVVMVGEIRDLET
AEIAVKAAQTGHLVLSTLHTNSAAETVIRLANMGVEPFNLASSLSLIIAQRLARRLCKHCKIAVRPSALLQSQFAFQPNE
ILYEANAAGCNECTGGYSGRVGIYEVMAFNTELAEAIMQRASIHQIERLAKANGMQTLQESGLEKLREGITSFAELQRVL
YF

Nucleotide


Download         Length: 1689 bp        

>NTDB_id=349232 EZZ25_RS06355 WP_000957192.1 1298208..1299896(-) (pilB) [Vibrio cholerae strain 20000]
ATGCTCACCAACCTTGTTGCTATCTTGCGTCAGGCTGAGTTAATCAGCGCGATGCAAGAACAAGCGGTGGTTACCCAGGT
TAGCGCTTCGGGGACGTCAGTACCCGAGGCGCTGCTTGGTTTAGGTTTTTTTCACCCCCGAGAACTCACCGAACAACTGA
GCCATATTTTCGGCTTGCAGGAAACCGACCTTAGCCGCTACGACTACGCCAACTTGTGCCAACAGCTCGGGCTGCGTGAA
CTGATCACCCGCTACGATGCCTTGCCGATTGCCAAGCAAGGCAATTTACTGCTGCTGGCGGTCTCTGACCCAACCTTACT
GCAAGCCGAAGAAGAGTTTCGTTTTGCGACTGGGCTGCAAGTCGAACTGGCACTGGCCGATCACCGCGCGCTGCAAGCGG
CGATTCGCCGTTTGTATGGCAGATCTATTCAAGGCGCAGCCAACCAAGGGAAAGAGATCAGCCAAGATGAGCTCGCCAAT
CTGGTTAAAGTCAGTGATGACGAGCTGCAATCGATTGAAGATCTCAGCCAAGATGACTCGCCGGTTAGCCGCTTTATCAA
CCAAGTGCTGCTCGATGCGGTACGTAAAGGTGCCTCGGATATTCATTTTGAGCCTTATGAAAACCAGTATCGGATCCGCC
TGCGCTGCGATGGGATCTTGGTCGAAACTCAGCAACCGGCTAGCCATTTAAGCCGCCGTTTAGCCGCGCGGATTAAAATT
CTCTCCAAATTAGATATTGCCGAGCGCCGCTTGCCACAAGATGGCCGGATTAAACTGCGCCTAAGCCGCGATACCGCCAT
TGATATGCGTGTATCCACGCTTCCCACTTTATGGGGAGAAAAAATCGTGCTGCGTCTGCTCGATAGCAGCGCCGCCAATC
TGGATATTGATAAGCTCGGCTATAACCCGCAGCAAAAGCAGCTCTATCTCAATGCCCTCAAAAGACCGCAAGGGATGATC
TTGATGACCGGCCCCACCGGCAGCGGCAAAACCGTTTCGCTCTATACTGGGCTGCGCATTCTCAACACGTCACAGATCAA
TATCTCCACCGCAGAAGATCCGGTAGAAATTAACCTCTCTGGGATTAACCAAGTGCAAGTGCAGCCGAAAATCGGCTTTG
GCTTTGCCGAAGCGCTACGCTCGTTTCTGCGCCAAGACCCGGATGTGGTGATGGTCGGCGAAATACGCGATCTGGAAACC
GCCGAAATCGCGGTCAAAGCCGCGCAAACCGGCCACTTGGTGCTTTCAACACTGCACACCAACTCGGCCGCTGAAACCGT
GATTCGCTTAGCCAATATGGGGGTGGAGCCGTTTAACCTCGCGTCCTCACTCAGTTTAATCATCGCCCAACGCCTCGCGC
GCCGCTTGTGTAAACATTGCAAAATCGCGGTGCGCCCTTCCGCCCTATTGCAAAGCCAATTTGCATTTCAGCCCAATGAA
ATCTTGTATGAAGCCAATGCGGCGGGGTGTAACGAGTGTACGGGCGGCTATTCAGGGCGCGTTGGGATCTATGAAGTGAT
GGCGTTTAATACCGAGCTGGCGGAGGCCATTATGCAACGCGCCAGCATTCATCAAATTGAACGTTTAGCCAAAGCCAATG
GCATGCAAACGTTGCAAGAGTCCGGCCTTGAAAAGCTGCGCGAAGGCATCACCAGCTTTGCCGAGCTGCAGCGTGTGCTC
TATTTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(39-141)

Type II/IV secretion system protein (T2SSE)

(188-455)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

98.754

100

0.988
  pilB Vibrio parahaemolyticus RIMD 2210633

74.021

100

0.74
  pilB Vibrio campbellii strain DS40M4

73.843

100

0.738
  pilB Legionella pneumophila strain ERS1305867

52.642

90.925

0.479
  pilB Acinetobacter baylyi ADP1

50.192

92.527

0.464
  pilB Acinetobacter baumannii D1279779

50

92.527

0.463
  pilF Neisseria gonorrhoeae MS11

45.73

100

0.457
  pilB/pilB1 Synechocystis sp. PCC 6803

36.65

100

0.393
  pilF Thermus thermophilus HB27

39.483

96.441

0.381
  pilB Haemophilus influenzae 86-028NP

39.469

93.772

0.37

Multiple sequence alignment