Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   EZZ25_RS06350 Genome accession   NZ_CP036499
Coordinates   1296939..1298165 (-) Length   408 a.a.
NCBI ID   WP_000648513.1    Uniprot ID   -
Organism   Vibrio cholerae strain 20000     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1291939..1303165
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EZZ25_RS06320 (EZZ25_06320) parC 1291940..1294225 (+) 2286 WP_000102513.1 DNA topoisomerase IV subunit A -
  EZZ25_RS06325 (EZZ25_06325) yacG 1294274..1294471 (-) 198 WP_000162865.1 DNA gyrase inhibitor YacG -
  EZZ25_RS06335 (EZZ25_06335) zapD 1294629..1295369 (-) 741 WP_000207194.1 cell division protein ZapD -
  EZZ25_RS06340 (EZZ25_06340) coaE 1295401..1296009 (-) 609 WP_000011556.1 dephospho-CoA kinase -
  EZZ25_RS06345 (EZZ25_06345) pilD 1296006..1296881 (-) 876 WP_000418751.1 A24 family peptidase Machinery gene
  EZZ25_RS06350 (EZZ25_06350) pilC 1296939..1298165 (-) 1227 WP_000648513.1 type II secretion system F family protein Machinery gene
  EZZ25_RS06355 (EZZ25_06355) pilB 1298208..1299896 (-) 1689 WP_000957192.1 type IV-A pilus assembly ATPase PilB Machinery gene
  EZZ25_RS06360 (EZZ25_06360) - 1299896..1300381 (-) 486 WP_000649332.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  EZZ25_RS06365 (EZZ25_06365) nadC 1300626..1301516 (-) 891 WP_000665278.1 carboxylating nicotinate-nucleotide diphosphorylase -
  EZZ25_RS06370 (EZZ25_06370) ampD 1301814..1302359 (+) 546 WP_000567319.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  EZZ25_RS06375 (EZZ25_06375) fldB 1302510..1303028 (-) 519 WP_000690112.1 flavodoxin FldB -

Sequence

Protein


Download         Length: 408 a.a.        Molecular weight: 45150.43 Da        Isoelectric Point: 10.3424

>NTDB_id=349231 EZZ25_RS06350 WP_000648513.1 1296939..1298165(-) (pilC) [Vibrio cholerae strain 20000]
MKATQTLPLKNYRWKGINSNGKKVSGQMLAISEIEVRDKLKDQHIQIKKLKKGSVSLLARLTHRVKSKDITILTRQLATM
LTTGVPIVQALKLVGDNHRKAEMKSILTQITKSVEAGTPLSKAMRTASAHFDTLYVDLVETGEMSGNLPEVFERLATYRE
KSEQLRAKVIKALIYPSMVVLVALGVSYLMLTMVIPEFESMFKGFGAELPWFTQQVLKLSHWVQAYSLWAFIAIAAAIFG
LKALRKNSFQIRLKTSRLGLKFPIIGNVLAKASIAKFSRTLATSFAAGIPILASLKTTAKTSGNVHFETAINEVYRDTAA
GMPMYIAMRNTDAFPEMVLQMVMIGEESGQLDDMLNKVATIYEFEVDNTVDNLGKILEPLIIVFLGTVVGGLVVAMYLPI
FNLMSVLG

Nucleotide


Download         Length: 1227 bp        

>NTDB_id=349231 EZZ25_RS06350 WP_000648513.1 1296939..1298165(-) (pilC) [Vibrio cholerae strain 20000]
ATGAAAGCGACCCAAACCTTACCTCTGAAAAATTATCGCTGGAAAGGCATCAACAGCAACGGCAAAAAAGTTTCCGGCCA
GATGCTCGCCATCTCCGAAATCGAGGTGCGCGATAAGCTCAAAGATCAGCATATTCAGATCAAAAAACTCAAAAAAGGTA
GTGTGTCTCTTTTGGCACGCCTAACCCATCGCGTGAAAAGTAAAGATATTACGATTTTGACTCGGCAGTTGGCGACCATG
CTCACCACGGGCGTACCCATTGTGCAAGCCCTCAAGTTGGTGGGCGATAATCACCGTAAAGCTGAGATGAAATCGATTCT
GACGCAAATCACCAAAAGCGTGGAAGCGGGCACGCCGCTTTCCAAGGCGATGCGCACCGCCAGCGCCCATTTTGATACCT
TGTATGTCGACTTAGTGGAAACCGGAGAGATGTCCGGTAACTTACCTGAGGTGTTTGAGCGTTTGGCCACCTACCGCGAG
AAAAGCGAGCAGCTACGCGCCAAGGTGATCAAAGCGCTCATCTACCCCAGCATGGTAGTGTTGGTCGCGCTCGGAGTTTC
CTACTTAATGCTCACCATGGTCATCCCAGAATTTGAAAGCATGTTTAAAGGCTTTGGTGCTGAACTGCCTTGGTTTACGC
AGCAAGTGCTGAAACTCTCACACTGGGTACAGGCTTACAGTTTATGGGCATTTATCGCCATCGCAGCAGCCATTTTTGGC
TTGAAAGCGCTGCGTAAAAACTCTTTCCAGATCCGTTTAAAAACCAGCCGCTTAGGGCTGAAATTTCCGATTATTGGTAA
TGTGCTCGCTAAGGCTTCCATCGCCAAATTCAGCCGCACCCTCGCTACCAGCTTTGCCGCGGGGATCCCAATTCTCGCCA
GTTTAAAAACCACCGCCAAAACCTCCGGCAATGTGCACTTTGAAACCGCGATTAATGAGGTGTACCGCGATACCGCTGCG
GGTATGCCGATGTACATTGCCATGCGCAATACCGACGCTTTTCCCGAAATGGTGCTGCAAATGGTGATGATCGGTGAAGA
GTCTGGGCAATTAGACGACATGCTCAACAAGGTCGCGACCATCTATGAATTTGAAGTCGATAACACGGTCGATAACTTGG
GCAAGATTCTTGAACCACTGATCATCGTCTTTCTTGGGACGGTTGTGGGCGGCTTAGTGGTGGCGATGTACTTACCTATC
TTTAACTTAATGAGTGTATTGGGTTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(277-399)

Type II secretion system (T2SS), protein F (T2SSF)

(74-196)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Vibrio cholerae strain A1552

99.51

100

0.995
  pilC Vibrio campbellii strain DS40M4

73.75

98.039

0.723
  pilC Acinetobacter baumannii D1279779

42.647

100

0.426
  pilC Acinetobacter baylyi ADP1

42.015

99.755

0.419
  pilC Legionella pneumophila strain ERS1305867

42.211

97.549

0.412
  pilC Pseudomonas stutzeri DSM 10701

42.172

97.059

0.409
  pilG Neisseria gonorrhoeae MS11

41.045

98.529

0.404
  pilG Neisseria meningitidis 44/76-A

40.547

98.529

0.4

Multiple sequence alignment