Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   EZZ25_RS06345 Genome accession   NZ_CP036499
Coordinates   1296006..1296881 (-) Length   291 a.a.
NCBI ID   WP_000418751.1    Uniprot ID   -
Organism   Vibrio cholerae strain 20000     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1291006..1301881
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EZZ25_RS06320 (EZZ25_06320) parC 1291940..1294225 (+) 2286 WP_000102513.1 DNA topoisomerase IV subunit A -
  EZZ25_RS06325 (EZZ25_06325) yacG 1294274..1294471 (-) 198 WP_000162865.1 DNA gyrase inhibitor YacG -
  EZZ25_RS06335 (EZZ25_06335) zapD 1294629..1295369 (-) 741 WP_000207194.1 cell division protein ZapD -
  EZZ25_RS06340 (EZZ25_06340) coaE 1295401..1296009 (-) 609 WP_000011556.1 dephospho-CoA kinase -
  EZZ25_RS06345 (EZZ25_06345) pilD 1296006..1296881 (-) 876 WP_000418751.1 A24 family peptidase Machinery gene
  EZZ25_RS06350 (EZZ25_06350) pilC 1296939..1298165 (-) 1227 WP_000648513.1 type II secretion system F family protein Machinery gene
  EZZ25_RS06355 (EZZ25_06355) pilB 1298208..1299896 (-) 1689 WP_000957192.1 type IV-A pilus assembly ATPase PilB Machinery gene
  EZZ25_RS06360 (EZZ25_06360) - 1299896..1300381 (-) 486 WP_000649332.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  EZZ25_RS06365 (EZZ25_06365) nadC 1300626..1301516 (-) 891 WP_000665278.1 carboxylating nicotinate-nucleotide diphosphorylase -

Sequence

Protein


Download         Length: 291 a.a.        Molecular weight: 32447.72 Da        Isoelectric Point: 7.1618

>NTDB_id=349230 EZZ25_RS06345 WP_000418751.1 1296006..1296881(-) (pilD) [Vibrio cholerae strain 20000]
MELFYFYPWLFPVLATLFGLIVGSFLNVVIYRLPKIMEREWRAECAASFPEYGITPPEGKLTLSLPRSTCPHCQTPIRVI
DNIPLLSWLALRGQCSHCKAPISARYPLIELLTALMSLVIATHFPFGVFAVALLFFTYVLIAATFIDFDTLLLPDQLTLP
LLWGGIALALLGFSPVSLSDAVIGAMAGYLSLWSIYWLFKLLTGKEGMGYGDFKLLAALGAWLGWQQLPVIVLLSSVVGV
IFGLIQLRQQKKGIDMAFPFGPYLAIAGWFALLWGDKVIDWYFTTWVGQPL

Nucleotide


Download         Length: 876 bp        

>NTDB_id=349230 EZZ25_RS06345 WP_000418751.1 1296006..1296881(-) (pilD) [Vibrio cholerae strain 20000]
ATGGAACTGTTTTACTTCTACCCTTGGCTGTTTCCGGTGCTCGCCACCCTGTTTGGCTTGATTGTCGGTAGCTTTCTCAA
TGTGGTGATCTACCGCTTACCCAAAATCATGGAGCGTGAATGGCGCGCGGAATGTGCGGCGAGTTTTCCTGAATATGGCA
TCACGCCACCAGAGGGTAAACTGACCCTCAGTTTGCCGCGTTCCACTTGCCCACACTGCCAAACGCCGATTCGCGTCATT
GATAATATCCCGCTACTGAGTTGGCTGGCGCTGCGCGGTCAATGCTCACACTGTAAAGCGCCGATCAGCGCTCGCTATCC
CTTGATTGAGCTACTCACTGCCCTCATGAGCTTAGTGATTGCTACTCACTTTCCGTTCGGGGTGTTTGCAGTGGCGCTGC
TGTTTTTCACCTATGTATTGATTGCGGCGACCTTCATCGACTTCGATACCCTGTTGCTGCCCGACCAATTAACGCTACCT
TTACTGTGGGGCGGTATTGCACTGGCACTGCTTGGTTTTTCTCCCGTTTCACTGAGTGATGCGGTGATCGGAGCCATGGC
GGGTTATCTCTCGCTGTGGTCGATTTACTGGCTATTTAAACTACTGACAGGTAAAGAAGGCATGGGTTATGGCGATTTCA
AATTGCTCGCCGCACTCGGCGCTTGGCTGGGTTGGCAGCAGTTGCCTGTCATCGTACTGCTCTCATCGGTGGTCGGGGTG
ATTTTCGGTTTAATCCAACTGCGCCAGCAGAAAAAAGGTATCGATATGGCCTTTCCGTTTGGCCCTTATCTCGCCATCGC
GGGGTGGTTTGCCTTGCTGTGGGGCGATAAGGTAATTGATTGGTACTTCACCACTTGGGTAGGACAACCGTTATGA

Domains

Predicted by InterproScan.

Type IV leader peptidase family (Peptidase_A24)

(135-244)

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(17-124)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

99.656

100

0.997
  pilD Vibrio campbellii strain DS40M4

75.694

98.969

0.749
  pilD Acinetobacter nosocomialis M2

54.044

93.471

0.505
  pilD Acinetobacter baumannii D1279779

53.676

93.471

0.502
  pilD Neisseria gonorrhoeae MS11

45.139

98.969

0.447

Multiple sequence alignment