Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   GIX96_RS20025 Genome accession   NZ_CP045863
Coordinates   3984240..3985736 (-) Length   498 a.a.
NCBI ID   WP_001040684.1    Uniprot ID   Q8X6R2
Organism   Escherichia coli O157:H7 strain ATCC 43890     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3979240..3990736
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GIX96_RS20000 (GIX96_20000) ataR 3979629..3979895 (-) 267 WP_001277108.1 type II toxin-antitoxin system antitoxin AtaR -
  GIX96_RS20005 (GIX96_20005) livJ 3980045..3981148 (-) 1104 WP_001021996.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
  GIX96_RS20010 (GIX96_20010) rpoH 3981419..3982273 (-) 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
  GIX96_RS20015 (GIX96_20015) ftsX 3982518..3983576 (-) 1059 WP_001042018.1 permease-like cell division protein FtsX -
  GIX96_RS20020 (GIX96_20020) ftsE 3983569..3984237 (-) 669 WP_000617723.1 cell division ATP-binding protein FtsE -
  GIX96_RS20025 (GIX96_20025) pilA 3984240..3985736 (-) 1497 WP_001040684.1 signal recognition particle-docking protein FtsY Machinery gene
  GIX96_RS20030 (GIX96_20030) rsmD 3985886..3986482 (+) 597 WP_000743199.1 16S rRNA (guanine(966)-N(2))-methyltransferase -
  GIX96_RS20035 (GIX96_20035) yhhL 3986472..3986741 (+) 270 WP_001295207.1 DUF1145 family protein -
  GIX96_RS20040 (GIX96_20040) yhhM 3986744..3987103 (-) 360 WP_000042873.1 DUF2500 domain-containing protein -
  GIX96_RS20045 (GIX96_20045) yhhN 3987244..3987870 (+) 627 WP_000964730.1 lysoplasmalogenase -
  GIX96_RS20050 (GIX96_20050) zntA 3987944..3990142 (+) 2199 WP_000106562.1 Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase ZntA -
  GIX96_RS20055 (GIX96_20055) tusA 3990244..3990489 (-) 246 WP_000130621.1 sulfurtransferase TusA -

Sequence

Protein


Download         Length: 498 a.a.        Molecular weight: 54730.53 Da        Isoelectric Point: 4.1468

>NTDB_id=348307 GIX96_RS20025 WP_001040684.1 3984240..3985736(-) (pilA) [Escherichia coli O157:H7 strain ATCC 43890]
MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQTVVEEIVQAQEPVKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVAE
NEKAQPEAEVVAQPEPVVEETPEPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
AAEAAEEAVMVVPPAEEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISLFRGKKIDDDLFEELEEQLLIAD
VGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEMGEILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQ
QGKSVMLAAGDTFRAAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKSHLMEELKK
IVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLDGTAKGGVIFSVADQFGIPIRYIGVGERIED
LRPFKADDFIEALFARED

Nucleotide


Download         Length: 1497 bp        

>NTDB_id=348307 GIX96_RS20025 WP_001040684.1 3984240..3985736(-) (pilA) [Escherichia coli O157:H7 strain ATCC 43890]
ATGGCGAAAGAAAAAAAACGTGGCTTTTTTTCCTGGCTGGGCTTTGGTCAAAAAGAGCAGACCCCGGAAAAAGAGACAGA
AGTTCAGAATGAACAAACGGTTGTAGAAGAAATCGTTCAGGCGCAAGAGCCTGTGAAGGCTTCTGAACAAGCCGTCGAAG
AGCAGCCGCAGGCGCATACTGAGGCCGAGGCGGAAACTTTTGCTGCCGACGTTGTGGAAGTCACTGAACAGGTTGCTGAA
AATGAAAAAGCGCAGCCTGAAGCGGAAGTCGTTGCACAACCGGAACCGGTCGTAGAAGAAACGCCGGAGCCAGTGGCTAT
CGAACGTGAAGAGCTGCCGTTGCCGGAAGACGTCAACGCCGAAGAGGTTTCGCCAGAAGAGTGGCAGGCTGAAGCGGAAA
CCGTAGAGATTGTCGAAGCGGCGGAAGAAGAAGCGGCTAAAGAAGAAATCACCGACGAAGAGCTGGAAGCACAGGCGCTG
GCTGCCGAAGCAGCAGAAGAGGCGGTGATGGTGGTTCCTCCGGCAGAAGAAGAGCAGCCGGTGGAAGAAATCGCTCAGGA
GCAGGAAAAACCGACCAAAGAAGGTTTTTTCGCGCGCCTGAAACGCAGCCTGTTAAAAACCAAAGAAAATCTCGGTTCCG
GATTTATCAGCCTGTTCCGCGGTAAAAAAATCGACGATGATCTGTTTGAGGAGCTGGAAGAGCAGCTTTTGATCGCCGAT
GTGGGCGTGGAAACCACACGTAAAATTATCACCAATCTGACGGAAGGCGCATCCCGCAAGCAGCTTCGTGATGCCGAGGC
GCTCTATGGCCTGCTGAAAGAAGAGATGGGTGAGATTCTGGCGAAAGTCGATGAGCCGCTGAATGTTGAAGGTAAAACGC
CGTTCGTGATCCTGATGGTGGGCGTCAACGGTGTGGGTAAAACCACGACGATTGGTAAGCTGGCACGTCAGTTTGAGCAG
CAGGGTAAATCGGTGATGCTGGCGGCGGGCGATACTTTCCGTGCAGCAGCGGTTGAACAGCTTCAGGTCTGGGGGCAGCG
CAACAATATTCCGGTGATTGCCCAGCATACCGGGGCGGATTCCGCCTCTGTTATCTTCGACGCCATTCAGGCGGCTAAAG
CGCGTAATATCGACGTCCTGATTGCCGATACCGCCGGACGCCTGCAGAACAAATCGCACCTGATGGAAGAGTTGAAGAAA
ATCGTCCGCGTGATGAAGAAACTCGACGTTGAAGCGCCGCATGAAGTTATGCTGACTATCGATGCCAGCACCGGGCAGAA
CGCGGTAAGCCAGGCCAAACTGTTCCATGAAGCCGTTGGCTTAACCGGCATCACGCTAACGAAACTGGACGGCACGGCGA
AAGGCGGGGTAATTTTCTCGGTGGCTGACCAGTTTGGTATCCCTATCCGCTACATTGGTGTCGGCGAACGTATTGAGGAT
TTGCGTCCGTTTAAGGCGGACGACTTTATAGAGGCACTTTTTGCCCGAGAGGATTAA

Domains

Predicted by InterProScan.

SRP54-type protein, GTPase domain (SRP54)

(294-494)

SRP54-type protein, helical bundle domain (SRP54_N)

(211-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q8X6R2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

48.831

77.309

0.378

Multiple sequence alignment