Detailed information    

insolico Bioinformatically predicted

Overview

Name   comP   Type   Machinery gene
Locus tag   MSIP34_RS03060 Genome accession   NC_012969
Coordinates   627584..628003 (-) Length   139 a.a.
NCBI ID   WP_015829475.1    Uniprot ID   C6X9N0
Organism   Methylovorus glucosotrophus SIP3-4     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 622584..633003
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MSIP34_RS03035 (Msip34_0597) - 623210..624097 (-) 888 WP_015829471.1 polyprenyl synthetase family protein -
  MSIP34_RS03040 (Msip34_0598) - 624097..624330 (-) 234 WP_013441429.1 exodeoxyribonuclease VII small subunit -
  MSIP34_RS03045 (Msip34_0599) - 624497..625900 (+) 1404 WP_015829472.1 hypothetical protein -
  MSIP34_RS03050 (Msip34_0600) - 625966..626616 (-) 651 WP_015829473.1 alpha/beta hydrolase -
  MSIP34_RS03055 (Msip34_0601) - 626766..627530 (+) 765 WP_015829474.1 3',5'-cyclic-nucleotide phosphodiesterase -
  MSIP34_RS03060 (Msip34_0602) comP 627584..628003 (-) 420 WP_015829475.1 pilin Machinery gene
  MSIP34_RS03065 (Msip34_0603) - 628199..628720 (-) 522 WP_015829476.1 SRPBCC family protein -
  MSIP34_RS03070 (Msip34_0604) - 628783..629778 (-) 996 WP_238525822.1 beta-propeller fold lactonase family protein -
  MSIP34_RS03075 (Msip34_0605) - 629982..630503 (+) 522 WP_013441435.1 RNA pyrophosphohydrolase -
  MSIP34_RS03080 (Msip34_0606) asd 630530..631375 (-) 846 WP_041369926.1 archaetidylserine decarboxylase -
  MSIP34_RS03085 (Msip34_0607) ilvC 631529..632545 (-) 1017 WP_013441438.1 ketol-acid reductoisomerase -

Sequence

Protein


Download         Length: 139 a.a.        Molecular weight: 14320.39 Da        Isoelectric Point: 6.1785

>NTDB_id=34790 MSIP34_RS03060 WP_015829475.1 627584..628003(-) (comP) [Methylovorus glucosotrophus SIP3-4]
MKNAQQGFTLIELMIVVAIIGILAAVALPAYQDYTIRARVTEGLGLASAQKLTVGENIANAGGTIDATACRGVNTNVAGN
VSSLTCNAGVLVVTMNDKGRGVALTLTPTVADQNSPVAWSCSTTEASYNFVPAECRKTS

Nucleotide


Download         Length: 420 bp        

>NTDB_id=34790 MSIP34_RS03060 WP_015829475.1 627584..628003(-) (comP) [Methylovorus glucosotrophus SIP3-4]
ATGAAAAACGCACAGCAAGGTTTTACTCTTATCGAACTGATGATCGTGGTCGCGATTATTGGTATTTTGGCAGCGGTGGC
ATTGCCTGCTTATCAGGATTACACCATCCGCGCACGCGTCACAGAAGGTTTGGGCTTGGCATCTGCACAAAAGCTGACCG
TCGGTGAAAACATTGCCAATGCTGGCGGCACTATTGATGCAACCGCGTGCCGAGGCGTAAATACAAACGTGGCCGGCAAT
GTCTCAAGCCTCACCTGCAATGCAGGCGTCCTCGTTGTAACTATGAACGACAAGGGTCGTGGTGTAGCTCTGACACTCAC
GCCAACAGTTGCTGACCAGAACTCTCCAGTCGCTTGGTCATGCTCAACAACTGAAGCAAGCTATAACTTTGTGCCTGCTG
AATGCCGCAAAACATCGTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(37-136)

Prokaryotic N-terminal methylation motif (N_methyl)

(3-28)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB C6X9N0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comP Acinetobacter baylyi ADP1

52.703

100

0.561
  pilA Ralstonia pseudosolanacearum GMI1000

41.718

100

0.489
  pilA2 Legionella pneumophila strain ERS1305867

49.254

96.403

0.475
  pilA2 Legionella pneumophila str. Paris

49.254

96.403

0.475
  pilA Pseudomonas aeruginosa PAK

38.065

100

0.424
  pilA/pilAI Pseudomonas stutzeri DSM 10701

38.667

100

0.417
  pilA/pilA1 Eikenella corrodens VA1

41.135

100

0.417
  pilE Neisseria gonorrhoeae MS11

44.615

93.525

0.417
  pilA Acinetobacter baumannii strain A118

36.17

100

0.367

Multiple sequence alignment