Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   C9E88_RS01500 Genome accession   NZ_CP035934
Coordinates   330564..332276 (-) Length   570 a.a.
NCBI ID   WP_106983894.1    Uniprot ID   A0A7Y8U356
Organism   Acinetobacter cumulans strain WCHAc060092     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 325564..337276
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C9E88_RS01470 (C9E88_002800) - 325636..325959 (+) 324 WP_106983888.1 pyrimidine/purine nucleoside phosphorylase -
  C9E88_RS01475 (C9E88_002795) rlmB 326105..326854 (+) 750 WP_106983889.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  C9E88_RS01480 (C9E88_002790) - 326902..327777 (+) 876 WP_106983890.1 DMT family transporter -
  C9E88_RS01485 (C9E88_002785) coaE 327824..328432 (-) 609 WP_106983891.1 dephospho-CoA kinase -
  C9E88_RS01490 (C9E88_002780) pilD 328446..329309 (-) 864 WP_106983892.1 A24 family peptidase Machinery gene
  C9E88_RS01495 (C9E88_002775) pilC 329309..330535 (-) 1227 WP_106983893.1 type II secretion system F family protein Machinery gene
  C9E88_RS01500 (C9E88_002770) pilB 330564..332276 (-) 1713 WP_106983894.1 type IV-A pilus assembly ATPase PilB Machinery gene
  C9E88_RS01505 (C9E88_002765) tpiA 332555..333346 (+) 792 WP_106983895.1 triose-phosphate isomerase -
  C9E88_RS01510 (C9E88_002760) secG 333360..333689 (+) 330 WP_106983896.1 preprotein translocase subunit SecG -
  C9E88_RS01535 (C9E88_002735) rimP 334467..334991 (+) 525 WP_106983897.1 ribosome maturation factor RimP -
  C9E88_RS01540 (C9E88_002730) nusA 335027..336511 (+) 1485 WP_106983898.1 transcription termination factor NusA -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 63287.30 Da        Isoelectric Point: 4.7780

>NTDB_id=345143 C9E88_RS01500 WP_106983894.1 330564..332276(-) (pilB) [Acinetobacter cumulans strain WCHAc060092]
MTALQGSPRFTGFIRRLVDEGHISAENMQQAMLSAKKANQDIVAYLVSEIGLSGLTIAETISIEFAEPVFDISAYDASQI
IREGLDEKLITRFRVLPIFKHSNVLFVATSNPTNIDAIDAIRFSSKMQTEAIIVEHAKLEKLIEQQFAEESTFEFDDDFD
LDLDDGTSTPQPQDSEDEEKGEEAPIVKYINKLLVDAIRMGASDLHFEPYEKSYRVRYRVDGVLRNITNPPLQLANRLAS
RLKVMSQMDISEKRIPQDGRIKLKLSKTKSIDFRVNSLPTLFGEKLVLRILDPSSAMLGIDALGYEPEQKELFMQALDKP
QGMLLITGPTGSGKTVSLYTGLNILNREDTNISTAEDPVEINLEGINQVNVNNKVGLTFAAALKSFLRQDPDIVMVGEIR
DLETAEIAVKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCSQCKAPVEVPHQSLIELGF
TEEDLANPDFKLYQPVGCSECREGYKGRVGIYEVMKVTPEISRIIMEDGNAIQIADASDKAGFNNLRRSGLIKVMQGVTS
LQEVNRVTSE

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=345143 C9E88_RS01500 WP_106983894.1 330564..332276(-) (pilB) [Acinetobacter cumulans strain WCHAc060092]
ATGACAGCATTACAAGGTTCTCCACGATTTACAGGATTTATTCGTCGTTTAGTCGACGAAGGGCACATTTCTGCTGAAAA
CATGCAACAAGCAATGTTGAGTGCAAAAAAAGCCAACCAAGACATCGTAGCCTATCTTGTCAGCGAAATTGGGCTATCTG
GCCTCACCATTGCTGAAACGATCTCTATTGAATTCGCTGAACCTGTTTTTGATATTAGTGCTTACGATGCGAGCCAAATC
ATTCGTGAGGGCTTAGATGAAAAGCTCATTACGCGCTTTCGGGTCTTACCTATTTTTAAACATAGCAATGTTCTTTTTGT
TGCAACGAGTAACCCAACCAATATTGATGCAATTGATGCCATTCGATTCAGCAGTAAAATGCAAACCGAAGCCATTATTG
TTGAACATGCCAAACTAGAAAAACTAATCGAACAACAGTTTGCCGAAGAAAGCACCTTTGAATTTGATGATGATTTTGAT
TTAGATCTTGACGATGGAACCAGTACACCCCAGCCACAAGATTCTGAAGATGAAGAAAAAGGCGAAGAAGCACCAATTGT
TAAATATATTAACAAATTGCTGGTTGATGCGATTCGTATGGGCGCATCGGATTTACACTTCGAACCTTATGAAAAAAGTT
ATCGTGTCCGTTATCGTGTAGATGGTGTATTACGCAATATCACCAACCCTCCCCTACAATTGGCCAATCGCTTGGCATCA
CGTTTAAAAGTGATGTCACAGATGGATATTTCCGAAAAACGTATCCCACAAGATGGCCGTATTAAACTCAAGCTATCAAA
AACAAAATCCATCGACTTCCGTGTCAACTCACTCCCAACATTATTTGGTGAGAAACTGGTACTGCGTATTCTCGATCCTT
CTAGTGCCATGCTGGGGATTGATGCATTAGGTTATGAGCCTGAGCAAAAAGAATTGTTCATGCAAGCACTTGATAAACCC
CAAGGCATGCTACTCATCACAGGTCCAACAGGCTCGGGTAAAACCGTATCGCTCTATACGGGTTTAAATATTTTAAACCG
AGAGGACACCAATATTTCCACAGCCGAAGACCCTGTGGAAATTAACTTGGAAGGCATTAATCAGGTTAACGTCAATAACA
AAGTTGGTTTAACCTTCGCTGCAGCATTAAAGTCATTCCTGCGTCAAGATCCAGACATTGTCATGGTCGGTGAGATTCGT
GACCTTGAAACAGCAGAGATTGCGGTTAAAGCTGCGCAAACGGGCCACATGGTGATGTCAACACTGCATACCAACAGTGC
CCCAGAAACCCTAACCCGCTTACGCAACATGGGTGTACCTTCTTTTAATATTGCAACTTCAGTCAACTTAGTGATTGCAC
AGCGTTTGGCGCGTCGTCTTTGTTCACAATGTAAAGCACCCGTTGAAGTTCCACACCAAAGCTTAATCGAGCTTGGTTTT
ACCGAAGAAGATCTCGCAAATCCAGATTTTAAGCTTTATCAACCCGTCGGATGTTCAGAATGTCGAGAAGGCTATAAAGG
CCGTGTCGGTATTTATGAAGTAATGAAAGTGACACCTGAAATTTCTCGCATTATTATGGAAGATGGTAACGCCATACAAA
TTGCAGATGCCTCAGACAAAGCAGGGTTTAATAATTTACGCCGTTCTGGCCTCATTAAGGTTATGCAGGGGGTGACTTCC
TTACAAGAAGTCAATCGTGTAACCAGTGAATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(54-150)

Type II/IV secretion system protein (T2SSE)

(192-459)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7Y8U356

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

79.335

100

0.795
  pilB Acinetobacter baylyi ADP1

73.997

100

0.744
  pilF Neisseria gonorrhoeae MS11

50.89

98.596

0.502
  pilB Legionella pneumophila strain ERS1305867

52.39

95.439

0.5
  pilB Vibrio cholerae strain A1552

50.965

90.877

0.463
  pilB Vibrio parahaemolyticus RIMD 2210633

50.482

91.053

0.46
  pilB Vibrio campbellii strain DS40M4

49.421

90.877

0.449
  pilB/pilB1 Synechocystis sp. PCC 6803

33.987

100

0.365
  pilF Thermus thermophilus HB27

36.396

99.298

0.361

Multiple sequence alignment