Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   EWA64_RS05845 Genome accession   NZ_CP035921
Coordinates   1079335..1080561 (+) Length   408 a.a.
NCBI ID   WP_061895187.1    Uniprot ID   -
Organism   Vibrio cidicii strain 2423-01     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1074335..1085561
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EWA64_RS05825 ampD 1075395..1075964 (-) 570 WP_061895191.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  EWA64_RS05830 nadC 1076057..1076944 (+) 888 WP_061895190.1 carboxylating nicotinate-nucleotide diphosphorylase -
  EWA64_RS05835 - 1077160..1077624 (+) 465 WP_061895189.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  EWA64_RS05840 pilB 1077625..1079313 (+) 1689 WP_061895188.1 type IV-A pilus assembly ATPase PilB Machinery gene
  EWA64_RS05845 pilC 1079335..1080561 (+) 1227 WP_061895187.1 type II secretion system F family protein Machinery gene
  EWA64_RS05850 pilD 1080701..1081570 (+) 870 WP_061895186.1 A24 family peptidase Machinery gene
  EWA64_RS05855 coaE 1081572..1082180 (+) 609 WP_061895185.1 dephospho-CoA kinase -
  EWA64_RS05860 zapD 1082213..1082953 (+) 741 WP_039430452.1 cell division protein ZapD -
  EWA64_RS05865 yacG 1083038..1083232 (+) 195 WP_061895184.1 DNA gyrase inhibitor YacG -
  EWA64_RS05870 rplS 1083667..1084020 (-) 354 WP_039430454.1 50S ribosomal protein L19 -
  EWA64_RS05875 trmD 1084063..1084812 (-) 750 WP_061893453.1 tRNA (guanosine(37)-N1)-methyltransferase TrmD -
  EWA64_RS05880 rimM 1084840..1085388 (-) 549 WP_061893452.1 ribosome maturation factor RimM -

Sequence

Protein


Download         Length: 408 a.a.        Molecular weight: 45260.38 Da        Isoelectric Point: 10.3476

>NTDB_id=344980 EWA64_RS05845 WP_061895187.1 1079335..1080561(+) (pilC) [Vibrio cidicii strain 2423-01]
MKVNAPVQIKNYRWKGINSSGKKVSGDVLAISEIEVRDKLHAQHIKIRKIKKSSVSILTRMSHRVKSRDITLFTRQISTM
LATGVPILQALKLVADNHRKAEMKSILTNVSKAIEAGTPMSKALRTASHHFDGLYTDLVATGEQSGNLALVFERLALYRE
KSEELRSKVIKALIYPCMVVLVALGVSYLMLTQVIPEFRKMFVGFGAELPWFTQKVLDLSDFVQAYGSGFFIALISTIYL
MRSISRKSDQVKLKFARLSLRLPVLGGVFSKAAIAKFSRTLATSFSSGIPILTSLQTTSKTSGNLHYQYAIESVYRDTAA
GMPMYIAMRNSNAFPELVLQMVMIGEESGRLDDMLNKIATIYEFEVDNTVDNLGKILEPLIIVFLGVVVGGLVAAMYLPI
FNLMSVMG

Nucleotide


Download         Length: 1227 bp        

>NTDB_id=344980 EWA64_RS05845 WP_061895187.1 1079335..1080561(+) (pilC) [Vibrio cidicii strain 2423-01]
ATGAAGGTAAACGCGCCTGTGCAGATTAAAAATTACCGTTGGAAGGGCATCAATAGCTCAGGCAAAAAAGTGTCGGGTGA
TGTGTTAGCCATTAGTGAAATCGAAGTGCGTGACAAGCTGCATGCGCAGCACATCAAGATCCGCAAGATAAAAAAATCCA
GCGTCTCAATACTGACCCGCATGAGCCACCGAGTCAAAAGCCGCGACATCACTCTCTTTACCCGCCAAATCTCGACCATG
CTCGCCACGGGTGTTCCTATCCTACAAGCGCTAAAACTGGTCGCCGATAACCACCGCAAAGCGGAGATGAAGTCCATTTT
AACCAACGTGAGTAAAGCGATTGAAGCAGGCACACCAATGTCCAAAGCCTTACGAACCGCCAGCCATCACTTTGATGGGC
TGTACACGGATTTGGTCGCGACGGGCGAGCAGTCAGGCAACCTTGCGCTGGTATTCGAACGCTTGGCGCTCTATCGAGAA
AAAAGTGAAGAGCTGCGTTCCAAAGTGATCAAAGCATTGATTTATCCTTGCATGGTGGTGCTCGTCGCCTTGGGCGTCTC
TTATCTGATGCTAACGCAAGTGATTCCCGAGTTTCGCAAAATGTTTGTCGGCTTTGGTGCCGAGCTGCCTTGGTTTACGC
AGAAAGTGCTCGATTTATCGGACTTTGTCCAAGCTTACGGTAGCGGTTTTTTTATCGCCCTTATCAGCACCATTTACCTG
ATGCGCAGCATCAGTCGCAAGTCCGATCAGGTTAAACTGAAGTTTGCCCGCCTCAGCTTGAGGCTCCCGGTACTCGGTGG
CGTATTTTCCAAAGCGGCGATTGCCAAGTTCAGCCGTACATTAGCCACCAGTTTTAGCTCGGGCATCCCGATTCTAACCA
GCTTACAAACCACCAGCAAAACGTCAGGCAATCTGCACTATCAATACGCGATTGAAAGTGTTTATCGCGACACCGCCGCA
GGAATGCCGATGTACATTGCGATGCGCAACAGCAACGCGTTTCCTGAACTGGTGCTGCAAATGGTGATGATCGGCGAAGA
GTCGGGGCGTCTTGACGATATGCTCAATAAAATCGCGACCATCTATGAGTTCGAGGTGGACAACACCGTAGACAACCTCG
GTAAGATCCTCGAGCCGCTGATCATCGTTTTTCTCGGCGTGGTCGTCGGCGGTTTGGTCGCGGCCATGTACCTACCAATA
TTTAACTTAATGAGTGTTATGGGCTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(277-399)

Type II secretion system (T2SS), protein F (T2SSF)

(73-196)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Vibrio campbellii strain DS40M4

76.225

100

0.762
  pilC Vibrio cholerae strain A1552

74.265

100

0.743
  pilC Acinetobacter baumannii D1279779

39.951

100

0.4
  pilG Neisseria gonorrhoeae MS11

40.299

98.529

0.397
  pilG Neisseria meningitidis 44/76-A

40.05

98.529

0.395
  pilC Legionella pneumophila strain ERS1305867

39.506

99.265

0.392
  pilC Pseudomonas stutzeri DSM 10701

39.401

98.284

0.387
  pilC Acinetobacter baylyi ADP1

39.547

97.304

0.385

Multiple sequence alignment