Detailed information NTG0344981

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: EWA64_RS05850
• Genome accession: NZ_CP035921
• Coordinates: 1080701..1081570 (+)
• Length: 289 a.a.
• NCBI ID: WP_061895186.1
• Uniprot ID: A0A151KVX9
• Organism: Vibrio cidicii strain 2423-01
• Function: type IV pilus biogenesis and function (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1075701..1086570

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
EWA64_RS05830	nadC	1076057..1076944 (+)	888	WP_061895190.1	carboxylating nicotinate-nucleotide diphosphorylase	-
EWA64_RS05835	-	1077160..1077624 (+)	465	WP_061895189.1	prepilin-type N-terminal cleavage/methylation domain-containing protein	-
EWA64_RS05840	pilB	1077625..1079313 (+)	1689	WP_061895188.1	type IV-A pilus assembly ATPase PilB	Machinery gene
EWA64_RS05845	pilC	1079335..1080561 (+)	1227	WP_061895187.1	type II secretion system F family protein	Machinery gene
EWA64_RS05850	pilD	1080701..1081570 (+)	870	WP_061895186.1	A24 family peptidase	Machinery gene
EWA64_RS05855	coaE	1081572..1082180 (+)	609	WP_061895185.1	dephospho-CoA kinase	-
EWA64_RS05860	zapD	1082213..1082953 (+)	741	WP_039430452.1	cell division protein ZapD	-
EWA64_RS05865	yacG	1083038..1083232 (+)	195	WP_061895184.1	DNA gyrase inhibitor YacG	-
EWA64_RS05870	rplS	1083667..1084020 (-)	354	WP_039430454.1	50S ribosomal protein L19	-
EWA64_RS05875	trmD	1084063..1084812 (-)	750	WP_061893453.1	tRNA (guanosine(37)-N1)-methyltransferase TrmD	-
EWA64_RS05880	rimM	1084840..1085388 (-)	549	WP_061893452.1	ribosome maturation factor RimM	-
EWA64_RS05885	rpsP	1085403..1085651 (-)	249	WP_011079541.1	30S ribosomal protein S16	-

Sequence

Protein

• Length: 289 a.a.
• Molecular weight: 32361.59 Da
• Isoelectric Point: 7.8837

>NTDB_id=344981 EWA64_RS05850 WP_061895186.1 1080701..1081570(+) (pilD) [Vibrio cidicii strain 2423-01]
MDALHYYPWLYVGLAALFGLIVGSFLNVVIYRLPKMMELEWQRECAEAFPQYAIPTPKETLTLSTPRSTCPTCHTPIRIR
DNIPLLSWFLLKGRCHQCQSKISGRYPAVELLSATLCALVAWKFGYSLYSLALLFFTFTLIAATFIDLDTMLLPDQLTLP
LTWSGIALALLEISPVSLQNSVIGAMAGYLCLWSVYHLFRLMTGKEGMGYGDFKLLAALGAWLGWQSLLMIILLSSVVGL
IFGLIQLRLQKQGIEKAFPFGPYLAIAGWVALMWGESLMSWYFSVMMGA

Nucleotide

• Length: 870 bp

>NTDB_id=344981 EWA64_RS05850 WP_061895186.1 1080701..1081570(+) (pilD) [Vibrio cidicii strain 2423-01]
ATGGATGCATTGCACTACTATCCTTGGCTGTATGTTGGCCTAGCGGCGTTATTTGGCTTGATTGTCGGTAGCTTTCTCAA
CGTGGTTATTTATCGCTTGCCGAAAATGATGGAACTGGAATGGCAACGAGAATGTGCCGAGGCCTTTCCTCAATACGCCA
TCCCCACGCCGAAGGAAACTCTCACTCTGAGCACGCCGCGTTCCACTTGCCCAACGTGCCATACGCCGATTCGTATCCGC
GATAACATTCCGCTGCTAAGTTGGTTTTTGCTCAAAGGGCGCTGTCATCAGTGTCAAAGCAAGATCAGTGGCCGCTACCC
TGCGGTTGAGCTGCTCAGCGCAACCCTATGCGCCTTGGTGGCGTGGAAATTTGGCTACAGCCTTTACTCGCTGGCACTGC
TGTTTTTCACCTTTACCTTGATCGCTGCGACCTTTATCGATCTCGATACCATGCTATTGCCAGATCAACTGACCCTGCCT
TTAACTTGGTCCGGTATTGCCCTTGCGCTGCTGGAAATCAGCCCTGTCTCTTTGCAAAACTCGGTGATCGGCGCCATGGC
AGGCTACCTCTGCCTTTGGTCTGTCTATCACCTTTTCCGTCTGATGACAGGCAAAGAAGGCATGGGGTATGGTGATTTTA
AGTTATTGGCAGCGCTCGGTGCTTGGCTAGGCTGGCAATCACTTCTGATGATCATTTTGCTCTCTTCGGTGGTTGGGCTA
ATCTTTGGCCTGATCCAACTTCGCTTACAAAAACAGGGCATCGAAAAAGCGTTTCCTTTTGGCCCCTATCTCGCCATCGC
TGGCTGGGTCGCATTAATGTGGGGCGAGTCGTTAATGAGTTGGTATTTCAGCGTAATGATGGGGGCGTAA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (135-244)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (17-124)

3D structure

• AlphaFold DB: A0A151KVX9

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio campbellii strain DS40M4	74.74	100	0.747
pilD	Vibrio cholerae strain A1552	68.056	99.654	0.678
pilD	Acinetobacter nosocomialis M2	52.015	94.464	0.491
pilD	Acinetobacter baumannii D1279779	50.909	95.156	0.484
pilD	Neisseria gonorrhoeae MS11	46.377	95.502	0.443