Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   EPB55_RS06735 Genome accession   NZ_CP035731
Coordinates   1330338..1332026 (+) Length   562 a.a.
NCBI ID   WP_080538712.1    Uniprot ID   -
Organism   Vibrio vulnificus strain 2142-77     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1325338..1337026
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EPB55_RS06715 pdhR 1326977..1327744 (-) 768 WP_011079554.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  EPB55_RS06720 ampD 1328133..1328705 (-) 573 WP_103276771.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  EPB55_RS06725 nadC 1328798..1329685 (+) 888 WP_103276770.1 carboxylating nicotinate-nucleotide diphosphorylase -
  EPB55_RS06730 pilA 1329908..1330333 (+) 426 WP_072608924.1 pilin Machinery gene
  EPB55_RS06735 pilB 1330338..1332026 (+) 1689 WP_080538712.1 type IV-A pilus assembly ATPase PilB Machinery gene
  EPB55_RS06740 pilC 1332075..1333301 (+) 1227 WP_085761331.1 type II secretion system F family protein Machinery gene
  EPB55_RS06745 pilD 1333395..1334264 (+) 870 WP_011079548.1 A24 family peptidase Machinery gene
  EPB55_RS06750 coaE 1334266..1334874 (+) 609 WP_039554617.1 dephospho-CoA kinase -
  EPB55_RS06755 zapD 1334907..1335647 (+) 741 WP_011079546.1 cell division protein ZapD -
  EPB55_RS06760 yacG 1335710..1335904 (+) 195 WP_026060801.1 DNA gyrase inhibitor YacG -
  EPB55_RS06765 rplS 1336113..1336466 (-) 354 WP_011079544.1 50S ribosomal protein L19 -

Sequence

Protein


Download         Length: 562 a.a.        Molecular weight: 62431.41 Da        Isoelectric Point: 5.5349

>NTDB_id=344101 EPB55_RS06735 WP_080538712.1 1330338..1332026(+) (pilB) [Vibrio vulnificus strain 2142-77]
MQSNLATILRQANQLSLTQEQACRETIQASGVTAPEALLQLGFFQPDELTEQLSAIFGLPRTDLNQYDYQELCNQLGLRE
LITRHQALPLQKSNAGLILAVADPTNIQAEDDFRFATGVQVEIVLADCQELQAAIRRLYGKSIKQSQSSGLKEIHQDELA
NLVEVSDDEVESIEDLSQDESPVSRFINQILLDAVRKGASDIHFEPYENQYRVRLRCDGILVEVQQPPSHLSRRLSARIK
ILSKLDIAERRLPQDGRIKLRLNQETAIDMRVSTLPTLFGEKIVLRLLDSSSSSLDIDKLGYNPQQKQLYLDALRKPQGM
ILMTGPTGSGKTVSLYTGLSLLNRPEVNISTAEDPVEINLPGINQVQVKPKIGFGFAEALRSFLRQDPDIVMVGEIRDLE
TAEIAVKAAQTGHLVLSTLHTNSAAETIVRLSNMGIASFNLASSLSLIIAQRLARRLCSHCKQPHPYNATFELLGLKADD
LIYQADKQGCHHCTHGYSGRVGIYEVMPFDEQLAEAIVKGASVQSLEMLARQKGMMTLKDSGLEKLKQGITSLEELQRVL
YL

Nucleotide


Download         Length: 1689 bp        

>NTDB_id=344101 EPB55_RS06735 WP_080538712.1 1330338..1332026(+) (pilB) [Vibrio vulnificus strain 2142-77]
GTGCAATCCAACCTTGCCACGATTCTGCGTCAGGCCAACCAACTTAGCCTAACGCAAGAACAAGCGTGTCGAGAGACAAT
ACAAGCTTCTGGTGTGACAGCACCGGAAGCTTTGTTGCAACTGGGATTTTTCCAGCCGGATGAATTAACCGAGCAGCTAA
GCGCGATTTTTGGCTTACCACGCACTGACCTCAACCAATATGACTACCAAGAGCTGTGTAATCAACTTGGGTTACGTGAG
CTGATCACTCGTCACCAAGCACTGCCACTGCAAAAAAGCAACGCCGGTTTAATACTCGCAGTTGCAGACCCGACCAATAT
TCAAGCTGAAGATGATTTCCGTTTTGCCACAGGTGTGCAGGTGGAGATTGTTTTGGCTGATTGCCAAGAGCTGCAAGCGG
CGATTCGCAGACTCTATGGAAAGAGCATCAAACAGAGCCAAAGCTCTGGTTTAAAAGAGATTCACCAAGATGAGCTGGCG
AATTTGGTCGAGGTTTCAGATGACGAAGTCGAGTCTATTGAAGACTTAAGCCAAGATGAATCCCCCGTGAGCCGCTTTAT
CAACCAAATCCTGCTGGATGCGGTACGTAAAGGCGCGTCCGATATTCATTTTGAACCGTACGAAAACCAATACCGCGTGC
GATTGCGTTGCGATGGTATTTTGGTGGAAGTGCAGCAGCCCCCAAGCCATTTAAGCCGCCGTTTATCCGCGAGGATCAAG
ATCCTCTCCAAGTTGGATATTGCGGAAAGGCGTCTGCCACAAGATGGACGGATTAAGCTGCGCCTCAACCAAGAAACCGC
GATTGATATGCGCGTTTCCACCTTACCCACACTGTTTGGTGAAAAAATTGTGTTGCGACTTCTCGACAGCTCGAGCAGCA
GCTTGGATATTGATAAGTTAGGCTACAACCCGCAACAAAAGCAGCTCTACCTCGATGCCCTGCGCAAACCGCAAGGGATG
ATTTTGATGACGGGCCCAACAGGAAGCGGCAAAACCGTTTCGCTGTACACAGGCTTGAGTTTACTTAACCGGCCAGAAGT
GAATATCTCCACCGCGGAAGACCCAGTCGAGATTAACCTCCCAGGAATCAACCAAGTGCAAGTGAAACCCAAAATTGGTT
TTGGTTTTGCAGAAGCATTGCGTTCTTTTCTCCGTCAAGACCCGGATATCGTCATGGTGGGGGAAATTCGAGATTTGGAA
ACCGCCGAGATCGCCGTCAAAGCAGCCCAAACCGGTCACCTTGTGCTATCCACCTTACATACCAACTCGGCAGCTGAAAC
CATTGTTCGTCTATCTAACATGGGGATCGCCAGCTTTAACTTAGCTTCATCCTTGAGTTTGATCATTGCTCAGCGTCTGG
CTCGTCGGTTATGCAGCCATTGCAAGCAACCTCATCCATACAACGCAACATTTGAGTTACTCGGTCTAAAAGCTGACGAC
TTGATTTATCAAGCTGATAAACAGGGTTGCCATCATTGTACTCATGGTTATTCCGGTCGTGTCGGTATATACGAAGTCAT
GCCTTTTGATGAACAGCTTGCAGAGGCGATCGTTAAAGGCGCCTCGGTACAAAGCTTAGAGATGCTTGCTCGACAAAAAG
GCATGATGACACTCAAAGATTCCGGCCTTGAAAAACTCAAACAGGGCATCACCAGCTTAGAAGAGCTGCAACGCGTCCTC
TATTTGTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(38-141)

Type II/IV secretion system protein (T2SSE)

(189-456)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio campbellii strain DS40M4

76.512

100

0.765
  pilB Vibrio parahaemolyticus RIMD 2210633

75.979

100

0.76
  pilB Vibrio cholerae strain A1552

74.423

100

0.746
  pilB Legionella pneumophila strain ERS1305867

52.827

91.281

0.482
  pilB Acinetobacter baumannii D1279779

51.533

92.883

0.479
  pilB Acinetobacter baylyi ADP1

50.383

92.883

0.468
  pilF Neisseria gonorrhoeae MS11

45.31

100

0.456
  pilF Thermus thermophilus HB27

40.787

99.466

0.406
  pilB/pilB1 Synechocystis sp. PCC 6803

36.888

100

0.375
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.491

94.306

0.363

Multiple sequence alignment