Detailed information NTG0344103

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: EPB55_RS06745
• Genome accession: NZ_CP035731
• Coordinates: 1333395..1334264 (+)
• Length: 289 a.a.
• NCBI ID: WP_011079548.1
• Uniprot ID: Q56740
• Organism: Vibrio vulnificus strain 2142-77
• Function: type IV pilus biogenesis and function (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1328395..1339264

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
EPB55_RS06725	nadC	1328798..1329685 (+)	888	WP_103276770.1	carboxylating nicotinate-nucleotide diphosphorylase	-
EPB55_RS06730	pilA	1329908..1330333 (+)	426	WP_072608924.1	pilin	Machinery gene
EPB55_RS06735	pilB	1330338..1332026 (+)	1689	WP_080538712.1	type IV-A pilus assembly ATPase PilB	Machinery gene
EPB55_RS06740	pilC	1332075..1333301 (+)	1227	WP_085761331.1	type II secretion system F family protein	Machinery gene
EPB55_RS06745	pilD	1333395..1334264 (+)	870	WP_011079548.1	A24 family peptidase	Machinery gene
EPB55_RS06750	coaE	1334266..1334874 (+)	609	WP_039554617.1	dephospho-CoA kinase	-
EPB55_RS06755	zapD	1334907..1335647 (+)	741	WP_011079546.1	cell division protein ZapD	-
EPB55_RS06760	yacG	1335710..1335904 (+)	195	WP_026060801.1	DNA gyrase inhibitor YacG	-
EPB55_RS06765	rplS	1336113..1336466 (-)	354	WP_011079544.1	50S ribosomal protein L19	-
EPB55_RS06770	trmD	1336508..1337257 (-)	750	WP_011079543.1	tRNA (guanosine(37)-N1)-methyltransferase TrmD	-
EPB55_RS06775	rimM	1337285..1337833 (-)	549	WP_011079542.1	ribosome maturation factor RimM	-
EPB55_RS06780	rpsP	1337849..1338097 (-)	249	WP_011079541.1	30S ribosomal protein S16	-

Sequence

Protein

• Length: 289 a.a.
• Molecular weight: 32295.30 Da
• Isoelectric Point: 6.4630

>NTDB_id=344103 EPB55_RS06745 WP_011079548.1 1333395..1334264(+) (pilD) [Vibrio vulnificus strain 2142-77]
MDALQYYPWLYIGLASLFGLLVGSFLNVVIYRLPKIMELEWRQECAESFPEYNITPPTETLTLSTPRSSCPSCHTPIRVR
DNIPVFSWLALRGKCHHCQTKISARYPFVEALSAFLCGLVAWKFGYAPITVALIGFTLVLIAATFIDLDTMLLPDQLTLP
LTWTGIALALLEISPVSLQDSVFGAMAGYLCLWSVYHLFRLLTGKEGMGYGDFKLLAALGAWLGWQYLPMIILLSSVVGL
IFGLIQLRLQKQGIEMAFPFGPYLAIAGWVALMWGDSLMSWYLNYLIGA

Nucleotide

• Length: 870 bp

>NTDB_id=344103 EPB55_RS06745 WP_011079548.1 1333395..1334264(+) (pilD) [Vibrio vulnificus strain 2142-77]
ATGGACGCGTTACAATACTACCCTTGGCTTTATATTGGTTTAGCTTCCCTGTTTGGCTTACTGGTAGGCAGCTTTCTTAA
CGTCGTCATCTACCGTCTACCGAAAATAATGGAACTGGAATGGCGCCAAGAGTGTGCAGAGTCCTTTCCTGAATACAACA
TCACACCACCGACAGAAACCCTAACATTAAGCACGCCGCGTTCAAGTTGCCCTTCGTGCCATACCCCAATACGAGTTCGA
GATAATATTCCTGTCTTTAGTTGGCTAGCTCTAAGAGGAAAATGTCACCATTGCCAGACGAAAATCAGTGCTCGCTATCC
TTTTGTTGAAGCACTGAGCGCCTTCTTGTGCGGCCTAGTCGCTTGGAAGTTCGGTTATGCACCGATTACCGTAGCACTGA
TTGGTTTTACTTTGGTGTTGATCGCCGCCACCTTCATCGACTTAGATACCATGCTACTGCCTGATCAACTGACACTGCCG
CTGACGTGGACAGGCATCGCGCTAGCGTTACTAGAGATAAGCCCAGTATCTCTGCAAGATTCAGTGTTCGGTGCGATGGC
AGGCTATCTCTGCCTTTGGAGCGTTTATCACCTTTTCAGATTGCTGACAGGGAAAGAAGGCATGGGTTACGGAGACTTTA
AATTGCTTGCGGCACTTGGAGCTTGGCTCGGCTGGCAATATCTACCGATGATCATCTTACTTTCTTCGGTTGTCGGGCTG
ATCTTTGGTCTCATTCAACTCAGATTGCAAAAGCAAGGCATTGAGATGGCTTTTCCATTTGGGCCTTATCTGGCCATTGC
TGGTTGGGTCGCCTTAATGTGGGGAGATAGCTTGATGTCTTGGTATCTCAACTATCTTATAGGGGCGTAA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (137-244)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (17-124)

3D structure

• AlphaFold DB: Q56740

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio campbellii strain DS40M4	77.163	100	0.772
pilD	Vibrio cholerae strain A1552	71.528	99.654	0.713
pilD	Acinetobacter nosocomialis M2	54.348	95.502	0.519
pilD	Acinetobacter baumannii D1279779	54.348	95.502	0.519
pilD	Neisseria gonorrhoeae MS11	48	95.156	0.457