Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   ET950_RS10140 Genome accession   NZ_CP035690
Coordinates   2262476..2263699 (+) Length   407 a.a.
NCBI ID   WP_154124514.1    Uniprot ID   -
Organism   Grimontia hollisae strain 2013V-1029     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2257476..2268699
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ET950_RS10120 ampD 2258453..2258995 (-) 543 WP_114996177.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  ET950_RS10125 nadC 2259187..2260092 (+) 906 WP_154124512.1 carboxylating nicotinate-nucleotide diphosphorylase -
  ET950_RS10130 - 2260320..2260766 (+) 447 WP_114996179.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  ET950_RS10135 pilB 2260770..2262464 (+) 1695 WP_154124513.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ET950_RS10140 pilC 2262476..2263699 (+) 1224 WP_154124514.1 type II secretion system F family protein Machinery gene
  ET950_RS10145 pilD 2263808..2264677 (+) 870 WP_154124515.1 prepilin peptidase Machinery gene
  ET950_RS10150 coaE 2264681..2265304 (+) 624 WP_005502108.1 dephospho-CoA kinase -
  ET950_RS10155 zapD 2265294..2266034 (+) 741 WP_005502110.1 cell division protein ZapD -
  ET950_RS10160 yacG 2266069..2266266 (+) 198 WP_115660105.1 DNA gyrase inhibitor YacG -
  ET950_RS10165 mutT 2266421..2266825 (-) 405 WP_114996183.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 407 a.a.        Molecular weight: 44998.90 Da        Isoelectric Point: 10.0910

>NTDB_id=343744 ET950_RS10140 WP_154124514.1 2262476..2263699(+) (pilC) [Grimontia hollisae strain 2013V-1029]
MATSSHKLFSYQWRGVTSKGAKVKGQTLAYSEQEARQALQEQRIRLDKIKKRSPSSWVRRKNAANAKDITLFSRQMATML
EAGVPLGETLRLLKASAKKAEMKFILGQIYSHIEAGSSLAQAMKTSSPLFDQFYCDLVATGEQTGHLEQIFARICVYREK
SEETKAKVIKAMIYPSIVCFLAILVTVGMLIFVIPTFKDVFSSFGAELPWFTRKVLGISDAIQLYGIYMLLALFIAVACV
RLGVTKSDKFRLRASRFSLQIPVIGDVLTKATISRFTRTLATTFSAGIPLLNGLSAAGKTANNTHYQRVIEEVHKQTSGG
RPLHRAIKETGQFPDMMIQMVMIGEESGALDDMLNKVAGIYEADVDNTVDNLGKILEPMIIVVLGVLIGSLVVAMYLPMF
NLMSVMG

Nucleotide


Download         Length: 1224 bp        

>NTDB_id=343744 ET950_RS10140 WP_154124514.1 2262476..2263699(+) (pilC) [Grimontia hollisae strain 2013V-1029]
ATGGCCACCAGTTCCCATAAACTTTTCTCTTACCAGTGGCGCGGTGTGACGTCGAAAGGCGCCAAAGTCAAAGGCCAGAC
ACTGGCATACTCCGAGCAGGAAGCCAGACAGGCACTGCAGGAACAGCGGATCCGGCTGGATAAAATCAAAAAGCGCTCGC
CCTCTTCCTGGGTACGGCGTAAAAATGCTGCCAACGCCAAAGACATTACCCTTTTCTCGCGCCAGATGGCGACCATGCTG
GAAGCGGGCGTGCCGCTGGGAGAAACCCTCAGGCTGCTGAAAGCCAGCGCCAAAAAAGCAGAAATGAAATTTATCCTCGG
CCAGATATACAGCCACATTGAAGCAGGCTCGTCACTGGCGCAGGCCATGAAAACCAGCTCGCCTCTTTTTGACCAGTTCT
ACTGCGATCTGGTGGCAACCGGTGAACAGACCGGACATTTGGAGCAGATTTTTGCCCGTATCTGTGTCTACCGGGAAAAA
AGTGAGGAAACCAAAGCCAAAGTCATCAAAGCGATGATATATCCATCTATTGTCTGTTTTCTCGCGATCCTTGTTACCGT
CGGCATGCTGATCTTCGTGATCCCAACATTCAAGGATGTCTTTTCATCGTTTGGGGCCGAACTGCCCTGGTTTACCCGTA
AAGTGCTGGGCATATCAGATGCTATCCAGCTTTACGGCATTTATATGCTGTTGGCCCTGTTTATCGCTGTAGCCTGTGTT
CGGCTTGGCGTCACCAAAAGCGATAAATTCCGGCTCAGGGCCAGCCGGTTTTCCCTGCAAATCCCGGTGATCGGAGACGT
GCTGACCAAAGCCACCATCTCCCGTTTTACCCGCACCCTGGCCACCACATTCAGTGCCGGTATTCCCTTACTCAATGGCT
TATCGGCGGCCGGTAAAACCGCCAACAACACCCACTACCAACGGGTAATCGAAGAGGTCCATAAACAGACATCCGGCGGC
CGTCCGCTCCACCGCGCCATCAAAGAAACCGGACAGTTTCCCGATATGATGATCCAGATGGTGATGATTGGTGAAGAGTC
CGGCGCCTTGGATGACATGCTGAACAAAGTCGCCGGCATCTATGAGGCTGATGTTGATAACACGGTCGACAACCTTGGCA
AAATCCTTGAGCCGATGATCATCGTTGTGCTCGGGGTGTTGATAGGTTCCCTGGTGGTTGCCATGTATCTGCCTATGTTC
AACCTGATGAGCGTCATGGGATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(72-195)

Type II secretion system (T2SS), protein F (T2SSF)

(276-398)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Vibrio cholerae strain A1552

52.463

99.754

0.523
  pilC Vibrio campbellii strain DS40M4

51.106

100

0.511
  pilC Pseudomonas stutzeri DSM 10701

42.26

100

0.423
  pilC Acinetobacter baumannii D1279779

40.704

97.789

0.398
  pilC Acinetobacter baylyi ADP1

40

99.509

0.398
  pilG Neisseria gonorrhoeae MS11

39.9

98.526

0.393
  pilC Legionella pneumophila strain ERS1305867

38.614

99.263

0.383
  pilG Neisseria meningitidis 44/76-A

40.365

94.349

0.381

Multiple sequence alignment