Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ET950_RS10135 Genome accession   NZ_CP035690
Coordinates   2260770..2262464 (+) Length   564 a.a.
NCBI ID   WP_154124513.1    Uniprot ID   -
Organism   Grimontia hollisae strain 2013V-1029     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2255770..2267464
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ET950_RS10115 pdhR 2257372..2258145 (-) 774 WP_005502094.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  ET950_RS10120 ampD 2258453..2258995 (-) 543 WP_114996177.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  ET950_RS10125 nadC 2259187..2260092 (+) 906 WP_154124512.1 carboxylating nicotinate-nucleotide diphosphorylase -
  ET950_RS10130 - 2260320..2260766 (+) 447 WP_114996179.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  ET950_RS10135 pilB 2260770..2262464 (+) 1695 WP_154124513.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ET950_RS10140 pilC 2262476..2263699 (+) 1224 WP_154124514.1 type II secretion system F family protein Machinery gene
  ET950_RS10145 pilD 2263808..2264677 (+) 870 WP_154124515.1 prepilin peptidase Machinery gene
  ET950_RS10150 coaE 2264681..2265304 (+) 624 WP_005502108.1 dephospho-CoA kinase -
  ET950_RS10155 zapD 2265294..2266034 (+) 741 WP_005502110.1 cell division protein ZapD -
  ET950_RS10160 yacG 2266069..2266266 (+) 198 WP_115660105.1 DNA gyrase inhibitor YacG -
  ET950_RS10165 mutT 2266421..2266825 (-) 405 WP_114996183.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 564 a.a.        Molecular weight: 61993.49 Da        Isoelectric Point: 5.7614

>NTDB_id=343743 ET950_RS10135 WP_154124513.1 2260770..2262464(+) (pilB) [Grimontia hollisae strain 2013V-1029]
MNSNLAVALRRAGYISPAQEQEVIAKVKSEGVSAPSALLALHILSSEALANTVSELFSEPLHSIKDYPWENTSQFVNQQD
LVLSSQALPLEIKHHQLFLGLSDPTRLDIQDDFQFATGYSIVPVLLEHAQIEGAIRRLYGKNVTERVKTSGVSDSELEAL
STNSDEITDTDDLFSDDAPVSRYIQQVLLDAVRKQASDIHFEPFEQRYQIRFRLDGILQTHSTPPVGISRRLSTRLKIIA
KLNIAERRLPQDGRIKLNLSENKSIDMRVSTLPTLWGEKVVLRILDGGSVALDIDKLGYSQQQKDDYLAALDRPQGMILM
TGPTGSGKTVSLYTGLKYLNTAERNISTAEDPVEINLPGINQVNINNDIGLDFAKALRAFLRQDPDVVMVGEIRDLETAE
IGIKAAQTGHLVLSTLHTNSAAESVTRLSNMGVAPYNLAASLTLIIAQRLGRRLCQHCKVPHPPGDRHLTALTQHYPSLR
PEQVYQASPEGCDHCNNGYLGRVGIYEVMPITRELAEAIGNGASASRIETLAMGNGMLTLPLSGIEKLNEGITSFAELQR
VISL

Nucleotide


Download         Length: 1695 bp        

>NTDB_id=343743 ET950_RS10135 WP_154124513.1 2260770..2262464(+) (pilB) [Grimontia hollisae strain 2013V-1029]
ATGAATTCCAACCTTGCTGTCGCCCTTCGCCGGGCTGGATATATTTCCCCGGCGCAGGAGCAAGAGGTGATCGCCAAAGT
CAAATCCGAGGGTGTCAGCGCACCGTCGGCTTTGCTCGCTTTACATATTCTGAGCAGTGAGGCGTTGGCAAACACGGTAT
CCGAGCTTTTCAGTGAGCCGCTGCACAGTATTAAAGATTACCCCTGGGAAAACACCAGCCAGTTTGTCAACCAACAGGAT
CTGGTGCTCTCCAGCCAGGCCCTGCCGCTGGAGATTAAACACCACCAGCTGTTTCTGGGCCTCAGCGACCCTACCCGCCT
GGATATCCAGGATGATTTCCAGTTTGCCACCGGTTATTCGATTGTGCCGGTGCTGCTGGAACATGCCCAGATAGAAGGAG
CGATTCGCCGCCTGTACGGCAAAAATGTCACAGAACGGGTCAAAACCAGCGGCGTCTCGGATTCCGAACTGGAAGCGCTC
AGCACCAACAGTGATGAGATAACAGACACCGACGATCTGTTTTCCGATGATGCCCCGGTCAGCCGTTATATCCAGCAGGT
TCTTTTGGATGCGGTGCGCAAACAAGCCTCCGATATTCACTTTGAACCCTTTGAACAGCGCTACCAGATACGTTTCCGTC
TCGATGGCATTTTGCAAACCCACAGCACACCGCCGGTGGGGATCTCCCGCCGCCTTTCTACACGCCTGAAAATCATCGCC
AAGCTCAATATTGCCGAGCGCCGGCTGCCGCAGGATGGGAGAATCAAGCTCAACCTGTCGGAAAACAAATCCATCGATAT
GCGGGTCTCGACCCTGCCTACTCTGTGGGGAGAAAAAGTGGTGCTGCGAATCCTTGATGGCGGCAGTGTGGCACTGGATA
TCGACAAGCTGGGTTATAGCCAACAGCAAAAAGACGACTACCTTGCCGCCCTCGATCGCCCACAGGGGATGATCTTGATG
ACCGGGCCAACAGGCAGCGGGAAAACCGTCTCCTTGTATACCGGGCTGAAATACTTAAATACTGCCGAGCGCAATATCTC
TACCGCAGAAGACCCGGTAGAAATCAACCTGCCCGGTATCAACCAGGTCAATATCAACAACGATATCGGGCTTGATTTTG
CCAAGGCATTGCGGGCCTTCTTACGTCAGGACCCGGATGTGGTAATGGTGGGTGAGATCCGGGATTTAGAAACCGCAGAA
ATCGGCATTAAAGCAGCGCAAACCGGCCATTTGGTGCTTTCCACCCTTCACACCAACTCTGCCGCAGAGAGTGTCACACG
TCTGTCGAACATGGGGGTTGCCCCTTACAACCTGGCTGCTTCCCTGACCCTGATTATTGCCCAGCGTCTGGGACGCCGTC
TGTGCCAGCACTGCAAAGTCCCGCATCCCCCCGGCGATCGCCACCTGACGGCACTCACCCAACATTATCCTTCGTTGAGG
CCGGAGCAGGTTTACCAGGCATCGCCTGAAGGCTGTGACCATTGCAACAACGGCTATCTGGGACGGGTGGGAATTTATGA
GGTGATGCCCATCACCCGCGAGCTTGCCGAAGCCATTGGCAATGGCGCATCGGCATCACGAATTGAAACCCTCGCCATGG
GCAACGGCATGTTAACACTGCCGCTTTCCGGCATTGAAAAACTAAATGAAGGCATCACCAGCTTTGCTGAACTGCAACGC
GTGATCAGCCTGTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(38-141)

Type II/IV secretion system protein (T2SSE)

(186-453)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

58.584

100

0.587
  pilB Vibrio campbellii strain DS40M4

57.27

100

0.573
  pilB Vibrio parahaemolyticus RIMD 2210633

56.915

100

0.569
  pilB Acinetobacter baylyi ADP1

47.143

99.291

0.468
  pilB Legionella pneumophila strain ERS1305867

48.052

95.567

0.459
  pilB Acinetobacter baumannii D1279779

48.582

93.794

0.456
  pilF Neisseria gonorrhoeae MS11

43.542

96.099

0.418
  pilF Thermus thermophilus HB27

37.794

98.05

0.371

Multiple sequence alignment