Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   ET950_RS10145 Genome accession   NZ_CP035690
Coordinates   2263808..2264677 (+) Length   289 a.a.
NCBI ID   WP_154124515.1    Uniprot ID   -
Organism   Grimontia hollisae strain 2013V-1029     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2258808..2269677
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ET950_RS10125 nadC 2259187..2260092 (+) 906 WP_154124512.1 carboxylating nicotinate-nucleotide diphosphorylase -
  ET950_RS10130 - 2260320..2260766 (+) 447 WP_114996179.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  ET950_RS10135 pilB 2260770..2262464 (+) 1695 WP_154124513.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ET950_RS10140 pilC 2262476..2263699 (+) 1224 WP_154124514.1 type II secretion system F family protein Machinery gene
  ET950_RS10145 pilD 2263808..2264677 (+) 870 WP_154124515.1 prepilin peptidase Machinery gene
  ET950_RS10150 coaE 2264681..2265304 (+) 624 WP_005502108.1 dephospho-CoA kinase -
  ET950_RS10155 zapD 2265294..2266034 (+) 741 WP_005502110.1 cell division protein ZapD -
  ET950_RS10160 yacG 2266069..2266266 (+) 198 WP_115660105.1 DNA gyrase inhibitor YacG -
  ET950_RS10165 mutT 2266421..2266825 (-) 405 WP_114996183.1 8-oxo-dGTP diphosphatase MutT -
  ET950_RS10170 secA 2266915..2269641 (-) 2727 WP_040528218.1 preprotein translocase subunit SecA -

Sequence

Protein


Download         Length: 289 a.a.        Molecular weight: 31664.66 Da        Isoelectric Point: 6.2123

>NTDB_id=343745 ET950_RS10145 WP_154124515.1 2263808..2264677(+) (pilD) [Grimontia hollisae strain 2013V-1029]
MDWLFDSPGLYVFLASLFGLIVGSFLNVVIYRLPKMMESQWRQECHACFPQEMPAPSESEVFNLSLPRSHCPSCHAPVRA
IDNIPVLSWLILGGKCRSCKTNISVRYPLIEILTAVLSGVVAYTLSGYWSLAVIGATFVLVVLCFIDLDTLLLPDQMTLP
LMWAGILLALTGLSPVSLQDAVIGAMAGYLSLWSVFQLFKLLTGKEGMGYGDFKLLAALGAWLGWQALPMVILMASMAGA
IGGIITMRVKGTGKETPFSFGPYLAIAGWISLLWGNMILDWYLTSYLGY

Nucleotide


Download         Length: 870 bp        

>NTDB_id=343745 ET950_RS10145 WP_154124515.1 2263808..2264677(+) (pilD) [Grimontia hollisae strain 2013V-1029]
ATGGATTGGCTTTTCGACTCCCCGGGTCTTTACGTTTTCCTTGCCTCACTGTTCGGCCTGATTGTGGGCAGTTTCCTCAA
TGTGGTTATCTATCGTTTACCCAAAATGATGGAGAGCCAGTGGCGACAGGAATGCCACGCCTGTTTTCCGCAAGAGATGC
CTGCGCCCAGTGAAAGTGAGGTATTCAACCTCAGCCTGCCGCGTTCTCACTGTCCGTCATGCCATGCGCCCGTCCGCGCG
ATTGACAACATTCCGGTATTAAGCTGGCTGATCCTTGGCGGCAAGTGCCGTTCCTGCAAAACCAACATCAGCGTCCGCTA
TCCCTTGATTGAAATTCTGACTGCCGTGCTGTCAGGTGTTGTGGCCTATACGCTGTCAGGGTATTGGTCGCTTGCCGTGA
TTGGTGCCACCTTTGTGCTGGTTGTGCTCTGTTTTATCGACCTTGATACCCTGTTGCTGCCAGACCAGATGACACTGCCC
CTGATGTGGGCCGGTATTTTACTTGCGCTTACCGGTCTCTCCCCGGTTTCACTCCAGGATGCGGTGATCGGCGCGATGGC
GGGATACCTGTCACTGTGGAGCGTGTTCCAGCTGTTTAAGCTGCTGACGGGCAAGGAAGGGATGGGTTATGGCGATTTCA
AGCTCCTTGCAGCCCTTGGGGCCTGGCTGGGTTGGCAGGCATTGCCTATGGTTATTCTGATGGCGTCTATGGCTGGTGCG
ATTGGTGGCATCATCACAATGCGCGTGAAAGGCACAGGGAAAGAAACACCTTTTTCATTTGGCCCTTACCTGGCCATTGC
GGGCTGGATAAGTTTGCTGTGGGGCAATATGATCCTGGACTGGTATTTAACATCTTATCTGGGTTATTGA

Domains

Predicted by InterproScan.

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(17-124)

Type IV leader peptidase family (Peptidase_A24)

(138-243)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

61.938

100

0.619
  pilD Vibrio campbellii strain DS40M4

63.475

97.578

0.619
  pilD Neisseria gonorrhoeae MS11

50.903

95.848

0.488
  pilD Acinetobacter nosocomialis M2

49.462

96.54

0.478
  pilD Acinetobacter baumannii D1279779

49.462

96.54

0.478

Multiple sequence alignment