Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   EUB48_RS04095 Genome accession   NZ_CP035503
Coordinates   847428..848645 (-) Length   405 a.a.
NCBI ID   WP_142817737.1    Uniprot ID   A0A515D825
Organism   Rhodoferax sediminis strain CHu59-6-5     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 842428..853645
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EUB48_RS04065 (EUB48_04065) - 843487..844440 (+) 954 WP_142817732.1 ATP-binding protein -
  EUB48_RS04070 (EUB48_04070) - 844437..844925 (+) 489 WP_142817733.1 NUDIX domain-containing protein -
  EUB48_RS04075 (EUB48_04075) - 844947..845159 (-) 213 WP_142817734.1 DNA gyrase inhibitor YacG -
  EUB48_RS04080 (EUB48_04080) zapD 845194..845949 (-) 756 WP_142821081.1 cell division protein ZapD -
  EUB48_RS04085 (EUB48_04085) coaE 845973..846581 (-) 609 WP_142817735.1 dephospho-CoA kinase -
  EUB48_RS04090 (EUB48_04090) pilD 846586..847428 (-) 843 WP_142817736.1 prepilin peptidase Machinery gene
  EUB48_RS04095 (EUB48_04095) pilC 847428..848645 (-) 1218 WP_142817737.1 type II secretion system F family protein Machinery gene
  EUB48_RS04100 (EUB48_04100) pilB 848668..850404 (-) 1737 WP_142817738.1 type IV-A pilus assembly ATPase PilB Machinery gene
  EUB48_RS04105 (EUB48_04105) - 850524..851048 (-) 525 WP_142817739.1 low molecular weight protein-tyrosine-phosphatase -
  EUB48_RS04110 (EUB48_04110) - 851142..851888 (+) 747 WP_142821082.1 ABC transporter ATP-binding protein -
  EUB48_RS04115 (EUB48_04115) - 851885..853282 (+) 1398 WP_142817740.1 ABC transporter permease -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44322.28 Da        Isoelectric Point: 9.5393

>NTDB_id=342888 EUB48_RS04095 WP_142817737.1 847428..848645(-) (pilC) [Rhodoferax sediminis strain CHu59-6-5]
MATAATKNIKEFVFEWEGKDRNGKQVRGETRAAGENQVQASLRRQGVLANKIKKRKMRAGKRIRPKDMAIFTRQLATMMK
AGVPLLQAFDIVGRGNPNVSVTKLLNDIRTDVETGTSLSAAFRKFPMYFDNLYCNLVEAGEAAGILEQLLDRLAVYMEKT
EAIKSKIKSALMYPISVLVVAFVVVAVIMIFVIPAFKQVFSSFGADLPAPTLVVIAISEFFVSYWWLIFGGLGGGLYFFM
QAWKRNEKVQMFMDRLMLKLPIFGALVEKSCIARWTRTLATMFAAGVPLVEALDSVGGASGNSVYALATEKIQQEVSTGT
SLTAAMGNANLFPSMVLQMCAIGEESGSIDHMLSKAADFYEAEVDDMVAGLSSLMEPIIIVVLGVIIGGIVISMYLPIFK
LGQVV

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=342888 EUB48_RS04095 WP_142817737.1 847428..848645(-) (pilC) [Rhodoferax sediminis strain CHu59-6-5]
ATGGCGACTGCAGCGACCAAGAACATCAAGGAATTTGTCTTCGAATGGGAAGGCAAGGATCGTAATGGCAAGCAGGTTCG
CGGCGAGACCCGTGCCGCCGGCGAAAACCAGGTGCAGGCGTCCCTGCGCCGGCAGGGGGTCCTGGCCAACAAAATCAAGA
AGCGCAAGATGCGCGCGGGCAAGCGCATCCGCCCGAAAGACATGGCGATTTTCACGCGCCAGCTCGCCACCATGATGAAG
GCCGGCGTGCCACTGCTGCAGGCCTTCGACATCGTCGGGCGCGGCAACCCGAATGTCAGCGTCACCAAGCTGCTCAACGA
CATCCGCACCGACGTCGAGACCGGTACCTCGCTGAGCGCGGCGTTCCGCAAGTTTCCGATGTATTTCGACAACCTCTACT
GCAACCTGGTCGAAGCAGGCGAGGCCGCCGGTATCCTGGAGCAGTTGCTGGACCGGCTGGCGGTCTACATGGAAAAAACC
GAGGCCATCAAGTCCAAGATCAAGTCCGCGCTGATGTACCCGATCTCGGTGCTGGTGGTGGCATTTGTGGTGGTGGCCGT
GATCATGATTTTCGTGATTCCGGCGTTCAAGCAGGTGTTCAGCTCCTTTGGCGCCGATCTGCCGGCGCCCACGCTGGTGG
TGATCGCCATCAGCGAATTTTTCGTCTCGTATTGGTGGCTGATTTTTGGCGGCCTCGGCGGCGGCCTGTACTTTTTCATG
CAGGCCTGGAAGCGCAACGAAAAAGTGCAGATGTTCATGGACCGGCTGATGCTCAAGCTGCCGATTTTCGGCGCCCTGGT
CGAAAAATCCTGTATCGCGCGCTGGACCCGCACGCTGGCCACCATGTTCGCGGCCGGTGTCCCGCTGGTGGAAGCGCTCG
ACTCGGTGGGCGGTGCTTCGGGCAATTCGGTGTACGCCCTGGCGACGGAAAAAATCCAGCAGGAAGTCTCCACCGGCACC
AGCCTGACCGCGGCCATGGGCAACGCGAACCTGTTTCCGAGCATGGTGCTGCAGATGTGCGCCATTGGCGAAGAATCCGG
CTCCATCGACCACATGCTGAGCAAGGCAGCTGATTTTTACGAGGCCGAGGTGGACGACATGGTGGCCGGCCTGTCCAGCC
TGATGGAGCCCATCATCATCGTGGTCCTGGGCGTCATCATCGGTGGCATCGTGATCTCCATGTACCTGCCGATCTTCAAG
CTGGGCCAGGTTGTCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)

Type II secretion system (T2SS), protein F (T2SSF)

(275-397)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A515D825

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

54.208

99.753

0.541
  pilG Neisseria gonorrhoeae MS11

51.88

98.519

0.511
  pilG Neisseria meningitidis 44/76-A

51.629

98.519

0.509
  pilC Legionella pneumophila strain ERS1305867

51.508

98.272

0.506
  pilC Acinetobacter baylyi ADP1

49.749

98.272

0.489
  pilC Acinetobacter baumannii D1279779

49.37

98.025

0.484
  pilC Vibrio cholerae strain A1552

40.049

100

0.402
  pilC Thermus thermophilus HB27

38.647

100

0.395
  pilC Vibrio campbellii strain DS40M4

39.698

98.272

0.39

Multiple sequence alignment