Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   ET464_RS20715 Genome accession   NZ_CP035492
Coordinates   1848505..1849509 (-) Length   334 a.a.
NCBI ID   WP_341869766.1    Uniprot ID   -
Organism   Paenibacillus protaetiae strain FW100M-2     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1843505..1854509
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ET464_RS08410 (ET464_08410) - 1843953..1844435 (+) 483 WP_129440006.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  ET464_RS08415 (ET464_08415) - 1844441..1844923 (+) 483 WP_165279953.1 hypothetical protein -
  ET464_RS08420 (ET464_08420) - 1844926..1845702 (-) 777 WP_129440010.1 A24 family peptidase -
  ET464_RS20570 (ET464_08425) - 1845780..1846145 (-) 366 WP_129444216.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  ET464_RS20290 - 1846213..1846470 (-) 258 WP_244226717.1 type II secretion system F family protein -
  ET464_RS08430 (ET464_08430) - 1846455..1847423 (-) 969 WP_244226718.1 type II secretion system F family protein -
  ET464_RS08435 (ET464_08435) - 1847439..1848488 (-) 1050 WP_129440012.1 type IV pilus twitching motility protein PilT -
  ET464_RS20715 pilF 1848505..1849509 (-) 1005 WP_341869766.1 GspE/PulE family protein Machinery gene
  ET464_RS20720 - 1849464..1850168 (-) 705 WP_341869767.1 hypothetical protein -
  ET464_RS08445 (ET464_08445) - 1850460..1851674 (+) 1215 WP_129440014.1 MFS transporter -
  ET464_RS08450 (ET464_08450) - 1851759..1852391 (-) 633 WP_129440016.1 O-methyltransferase -
  ET464_RS08455 (ET464_08455) - 1852585..1853721 (+) 1137 WP_129440018.1 class I SAM-dependent RNA methyltransferase -

Sequence

Protein


Download         Length: 334 a.a.        Molecular weight: 36579.08 Da        Isoelectric Point: 7.5737

>NTDB_id=342825 ET464_RS20715 WP_341869766.1 1848505..1849509(-) (pilF) [Paenibacillus protaetiae strain FW100M-2]
MHNVITARIKIMANLNVAERRVPQDGRVELEVDFRKIDVRIATLPTLHGEKIVIRILDLGHGLSSVSSLGFSEPNERRFL
QQIEASSGVVLITGPTGSGKSTTLYSALSHLNRDDVNIITVEDPVEYQLDGVNQVQVHHAAGMTFSRGLRAILRQDPNVI
MIGEIRDAETAEIAVRAAITGHLVLSTLHTNSAAGAVTRLADMGVEPFLISSSVRCVVAQRLVRKICPHCKTEYEPLPAE
IKRLSDHGLYTNRLFHGAGCGDCGRTGYKGRIAIHEVLEVDDAMRAMVLAKRSDHDYFAHALKQGMIPLMADGLRKAAEG
QTTLAEVYRVLGKE

Nucleotide


Download         Length: 1005 bp        

>NTDB_id=342825 ET464_RS20715 WP_341869766.1 1848505..1849509(-) (pilF) [Paenibacillus protaetiae strain FW100M-2]
ATGCATAATGTCATTACTGCCCGCATTAAAATTATGGCTAATTTGAACGTTGCCGAACGCCGGGTGCCCCAAGACGGGCG
CGTCGAACTCGAAGTGGACTTTCGCAAAATCGACGTCCGCATTGCTACGCTTCCTACCCTTCACGGCGAAAAAATCGTGA
TCCGCATCCTGGATCTCGGGCACGGCTTGTCCAGCGTGTCATCGCTAGGTTTCTCCGAACCCAATGAGCGCCGGTTTCTG
CAGCAGATTGAAGCTTCGAGCGGTGTAGTGCTCATTACCGGGCCAACCGGAAGCGGCAAGTCAACGACGCTGTATTCGGC
GCTGTCGCATTTGAACCGGGACGATGTCAACATTATTACAGTCGAAGATCCGGTCGAATACCAGCTGGACGGCGTTAACC
AGGTGCAGGTTCACCATGCGGCGGGCATGACCTTTTCCCGCGGGCTGCGCGCCATTTTGCGGCAGGATCCAAATGTTATT
ATGATCGGAGAAATCCGCGATGCGGAAACGGCGGAGATTGCGGTGCGGGCGGCTATAACCGGCCATCTTGTGCTCAGTAC
GCTGCACACCAATAGCGCGGCGGGAGCGGTTACGCGGCTGGCGGATATGGGGGTGGAACCGTTCCTTATTTCCTCGTCGG
TCCGGTGTGTAGTGGCGCAGCGGCTGGTTCGGAAAATATGCCCGCACTGCAAAACGGAGTACGAGCCTTTGCCGGCAGAA
ATAAAGCGGTTATCGGACCACGGTCTCTATACAAACCGCCTGTTTCATGGCGCGGGCTGCGGAGATTGCGGGCGGACAGG
CTATAAAGGGCGAATCGCTATCCATGAGGTGCTGGAGGTAGATGATGCGATGCGCGCAATGGTGTTGGCGAAACGTTCGG
ACCATGATTATTTCGCCCATGCGCTTAAGCAGGGCATGATTCCGCTTATGGCGGACGGGCTGCGCAAAGCGGCTGAAGGC
CAGACGACGCTGGCGGAAGTATACCGCGTCCTTGGCAAGGAATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(4-225)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Thermus thermophilus HB27

51.376

97.904

0.503
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.848

98.503

0.491
  pilB Vibrio cholerae strain A1552

47.734

99.102

0.473
  pilB/pilB1 Synechocystis sp. PCC 6803

44.828

100

0.467
  pilB Legionella pneumophila strain ERS1305867

46.667

98.802

0.461
  pilB Vibrio campbellii strain DS40M4

46.526

99.102

0.461
  pilB Vibrio parahaemolyticus RIMD 2210633

46.224

99.102

0.458
  pilB Acinetobacter baumannii D1279779

44.706

100

0.455
  pilB Acinetobacter baylyi ADP1

44.91

100

0.449
  pilF Neisseria gonorrhoeae MS11

44.109

99.102

0.437
  pilB Haemophilus influenzae 86-028NP

42.47

99.401

0.422
  pilB Haemophilus influenzae Rd KW20

41.867

99.401

0.416
  pilB Glaesserella parasuis strain SC1401

39.577

99.102

0.392
  ctsE Campylobacter jejuni subsp. jejuni 81-176

39.275

99.102

0.389

Multiple sequence alignment