Detailed information    

insolico Bioinformatically predicted

Overview

Name   cinA   Type   Machinery gene
Locus tag   ETT63_RS08715 Genome accession   NZ_CP035436
Coordinates   1720110..1721381 (-) Length   423 a.a.
NCBI ID   WP_032459984.1    Uniprot ID   -
Organism   Streptococcus pyogenes strain emm89.14     
Function   require for competence (predicted from homology)   
Unclear

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 1710388..1740560 1720110..1721381 within 0


Gene organization within MGE regions


Location: 1710388..1740560
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ETT63_RS08665 (ETT63_08660) nrdG 1710388..1711002 (-) 615 WP_002982219.1 anaerobic ribonucleoside-triphosphate reductase activating protein -
  ETT63_RS08670 (ETT63_08665) - 1711002..1711511 (-) 510 WP_136267864.1 GNAT family N-acetyltransferase -
  ETT63_RS08675 (ETT63_08670) - 1711520..1712455 (-) 936 WP_111676876.1 Gfo/Idh/MocA family protein -
  ETT63_RS09255 - 1712484..1712630 (-) 147 WP_011106921.1 hypothetical protein -
  ETT63_RS08680 (ETT63_08675) nrdD 1712812..1715010 (-) 2199 WP_002992831.1 anaerobic ribonucleoside-triphosphate reductase -
  ETT63_RS08685 (ETT63_08680) - 1715107..1716666 (-) 1560 WP_030126183.1 membrane protein -
  ETT63_RS08690 (ETT63_08685) - 1717079..1717384 (-) 306 WP_002982199.1 DUF1292 domain-containing protein -
  ETT63_RS08695 (ETT63_08690) ruvX 1717396..1717815 (-) 420 WP_111693462.1 Holliday junction resolvase RuvX -
  ETT63_RS08700 (ETT63_08695) - 1717812..1718081 (-) 270 WP_002982194.1 IreB family regulatory phosphoprotein -
  ETT63_RS08705 (ETT63_08700) spx 1718196..1718594 (-) 399 WP_002982188.1 transcriptional regulator Spx -
  ETT63_RS08710 (ETT63_08705) recA 1718885..1720021 (-) 1137 WP_002992179.1 recombinase RecA Machinery gene
  ETT63_RS08715 (ETT63_08710) cinA 1720110..1721381 (-) 1272 WP_032459984.1 competence/damage-inducible protein A Machinery gene
  ETT63_RS08720 (ETT63_08715) - 1721450..1722010 (-) 561 WP_023611248.1 DNA-3-methyladenine glycosylase I -
  ETT63_RS08725 (ETT63_08720) ruvA 1722020..1722616 (-) 597 WP_011285268.1 Holliday junction branch migration protein RuvA -
  ETT63_RS08730 (ETT63_08725) - 1722618..1723838 (-) 1221 WP_136267865.1 MFS transporter -
  ETT63_RS08735 (ETT63_08730) mutL 1723849..1725831 (-) 1983 WP_136267866.1 DNA mismatch repair endonuclease MutL -
  ETT63_RS08740 (ETT63_08735) - 1725926..1727077 (-) 1152 WP_023612650.1 site-specific integrase -
  ETT63_RS08745 (ETT63_08740) - 1727399..1727542 (+) 144 WP_011185066.1 putative holin-like toxin -
  ETT63_RS08750 (ETT63_08745) - 1728391..1729158 (-) 768 WP_115219873.1 helix-turn-helix transcriptional regulator -
  ETT63_RS08755 (ETT63_08750) - 1729312..1729518 (+) 207 WP_002992503.1 helix-turn-helix transcriptional regulator -
  ETT63_RS08765 (ETT63_08760) - 1729552..1730224 (+) 673 Protein_1621 BRO family protein -
  ETT63_RS08770 (ETT63_08765) - 1730234..1730839 (+) 606 WP_136267867.1 Bro-N domain-containing protein -
  ETT63_RS08775 (ETT63_08770) - 1730860..1731267 (+) 408 WP_002992501.1 hypothetical protein -
  ETT63_RS09425 - 1731280..1731402 (+) 123 WP_011285272.1 hypothetical protein -
  ETT63_RS08780 (ETT63_08775) - 1731685..1732206 (+) 522 WP_227875683.1 Rha family transcriptional regulator -
  ETT63_RS08790 (ETT63_08785) - 1732845..1733018 (+) 174 WP_000074663.1 plasmid mobilization protein -
  ETT63_RS08795 (ETT63_08790) - 1733204..1733608 (+) 405 WP_227868741.1 hypothetical protein -
  ETT63_RS08800 (ETT63_08795) - 1733710..1734042 (+) 333 WP_011285276.1 hypothetical protein -
  ETT63_RS08805 (ETT63_08800) - 1734042..1734233 (+) 192 WP_027968868.1 hypothetical protein -
  ETT63_RS08810 (ETT63_08805) - 1734246..1734608 (+) 363 WP_000206026.1 hypothetical protein -
  ETT63_RS08815 (ETT63_08810) - 1734605..1734934 (+) 330 WP_000174505.1 hypothetical protein -
  ETT63_RS08820 (ETT63_08815) - 1734937..1735209 (+) 273 WP_014407941.1 hypothetical protein -
  ETT63_RS08825 (ETT63_08820) - 1735210..1736067 (+) 858 WP_111694380.1 primase alpha helix C-terminal domain-containing protein -
  ETT63_RS08830 (ETT63_08825) - 1736036..1737724 (+) 1689 WP_136291319.1 phage/plasmid primase, P4 family -
  ETT63_RS08835 (ETT63_08830) - 1738011..1738184 (+) 174 WP_003045773.1 hypothetical protein -
  ETT63_RS09260 - 1738190..1738363 (+) 174 WP_164972313.1 hypothetical protein -
  ETT63_RS08840 (ETT63_08835) - 1738365..1738874 (+) 510 WP_109828907.1 hypothetical protein -
  ETT63_RS08845 (ETT63_08840) - 1738949..1739437 (+) 489 WP_136267846.1 hypothetical protein -
  ETT63_RS08855 (ETT63_08850) - 1739840..1740202 (+) 363 WP_003058730.1 DUF1492 domain-containing protein -
  ETT63_RS08860 (ETT63_08855) - 1740177..1740560 (+) 384 WP_023612665.1 ArpU family phage packaging/lysis transcriptional regulator -

Sequence

Protein


Download         Length: 423 a.a.        Molecular weight: 46018.75 Da        Isoelectric Point: 4.8322

>NTDB_id=341507 ETT63_RS08715 WP_032459984.1 1720110..1721381(-) (cinA) [Streptococcus pyogenes strain emm89.14]
MKAELIAVGTEILTGQIVNTNAQFLSEKMAELGIDVYFQTAVGDNEERLLSVITTASQRSDLVILCGGLGPTKDDLTKQT
LAKYLRRDLVYDEQACQKLDDFFAKRKPSSRTPNNERQAQVIEGSIPLPNKTGLAVGGFITVDGISYVVLPGPPSELKPM
VNEELVPLLSKQYSTLYSKVLRFFGIGESQLVTVLSDFIENQTDPTIAPYAKTGEVTLRLSTKTENQALADKKLGQLEAQ
LLSRKTLEGQPLADVFYGYGEDNSLARETFELLVKYDKTITAAESLTAGLFQSTLASFPGASQVFNGGFVTYSMEEKAKM
LGLPLEELKSHGVVSAYTAEGMAEQARLLTGADIGVSLTGVAGPDMLEEQPAGTVFIGLATQNKVESIKVLISGRSRLDV
RYIATLHAFNMVRKTLLKLENLL

Nucleotide


Download         Length: 1272 bp        

>NTDB_id=341507 ETT63_RS08715 WP_032459984.1 1720110..1721381(-) (cinA) [Streptococcus pyogenes strain emm89.14]
ATGAAAGCTGAACTGATTGCAGTAGGTACCGAAATTTTGACTGGTCAAATTGTGAATACCAATGCTCAATTTCTGTCGGA
AAAAATGGCAGAGCTAGGTATTGATGTCTATTTTCAAACGGCTGTTGGGGACAACGAGGAGCGTTTACTTTCAGTGATTA
CAACTGCTAGTCAGCGTAGTGACTTGGTCATTTTATGCGGTGGCCTTGGTCCAACGAAAGATGATTTAACCAAACAAACT
TTAGCAAAGTACCTTAGGAGAGACTTGGTTTATGATGAACAAGCTTGTCAGAAACTAGATGACTTTTTTGCTAAGCGCAA
GCCTTCATCACGGACACCAAATAATGAGCGACAGGCACAAGTGATTGAAGGGTCAATCCCTTTGCCAAATAAAACTGGTC
TTGCGGTTGGTGGGTTCATTACAGTCGATGGTATTAGTTATGTTGTCTTACCGGGTCCTCCAAGTGAATTGAAGCCGATG
GTAAATGAAGAATTGGTACCACTTCTGTCAAAACAATACAGTACATTGTATTCAAAGGTACTACGCTTTTTTGGTATTGG
GGAAAGTCAGTTGGTAACAGTCTTGTCAGATTTTATTGAGAATCAAACTGATCCAACCATTGCTCCGTATGCTAAGACTG
GCGAAGTGACTCTTCGCTTATCAACAAAAACTGAAAACCAAGCTCTGGCAGATAAAAAGTTAGGTCAGCTAGAAGCGCAG
CTACTATCCCGAAAAACTCTTGAAGGTCAACCCTTAGCTGATGTCTTTTATGGCTATGGGGAGGATAATTCCTTAGCGCG
TGAGACATTTGAGCTCTTAGTAAAATATGATAAGACAATTACAGCAGCAGAAAGTCTAACCGCGGGATTATTTCAGTCAA
CTTTGGCGAGTTTTCCAGGAGCTTCTCAAGTATTCAATGGAGGCTTTGTGACTTATAGCATGGAAGAAAAAGCGAAAATG
CTAGGCCTTCCTTTAGAGGAGTTGAAATCGCATGGCGTTGTTAGTGCTTATACAGCCGAGGGGATGGCGGAGCAAGCAAG
GTTATTGACTGGTGCTGATATTGGGGTAAGTTTAACAGGTGTTGCCGGACCAGATATGTTGGAGGAACAGCCTGCAGGTA
CAGTTTTCATTGGCCTTGCCACTCAAAATAAGGTAGAATCAATAAAGGTTTTGATTAGCGGGCGAAGTCGTTTGGATGTG
CGCTATATCGCTACTTTACATGCCTTTAATATGGTCCGTAAAACTTTATTAAAACTTGAGAATTTGCTATAA

Domains

Predicted by InterproScan.

Competence-damaged protein (CinA)

(263-416)

Probable molybdopterin binding domain (MoCF_biosynth)

(5-170)

Damage-inducible protein CinA KH domain (CinA_KH)

(178-243)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  cinA Streptococcus mutans UA159

69.976

100

0.7
  cinA Streptococcus mitis SK321

69.784

98.582

0.688
  cinA Streptococcus mitis NCTC 12261

69.305

98.582

0.683
  cinA Streptococcus pneumoniae TIGR4

68.345

98.582

0.674
  cinA Streptococcus pneumoniae Rx1

68.345

98.582

0.674
  cinA Streptococcus pneumoniae R6

68.345

98.582

0.674
  cinA Streptococcus pneumoniae D39

68.106

98.582

0.671
  cinA Streptococcus suis isolate S10

53.365

98.345

0.525
  cinA Bacillus subtilis subsp. subtilis str. 168

46.89

98.818

0.463

Multiple sequence alignment