Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   TOLA_RS07130 Genome accession   NC_012691
Coordinates   1543722..1544861 (+) Length   379 a.a.
NCBI ID   WP_041609767.1    Uniprot ID   -
Organism   Tolumonas auensis DSM 9187     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1538722..1549861
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  TOLA_RS07105 (Tola_1390) aroG 1539076..1540125 (+) 1050 WP_015878477.1 3-deoxy-7-phosphoheptulonate synthase AroG -
  TOLA_RS07110 (Tola_1391) hxpB 1540190..1540840 (-) 651 WP_015878478.1 hexitol phosphatase HxpB -
  TOLA_RS07115 (Tola_1392) - 1540973..1542331 (-) 1359 WP_015878479.1 MATE family efflux transporter -
  TOLA_RS07120 (Tola_1393) - 1542375..1542584 (-) 210 WP_015878480.1 YaeP family protein -
  TOLA_RS07125 (Tola_1394) - 1542656..1543525 (-) 870 WP_015878481.1 fructosamine kinase family protein -
  TOLA_RS07130 (Tola_1395) pilU 1543722..1544861 (+) 1140 WP_041609767.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  TOLA_RS07135 (Tola_1396) - 1544929..1545813 (+) 885 WP_015878483.1 DMT family transporter -
  TOLA_RS07140 (Tola_1397) rsuA 1545810..1546505 (-) 696 WP_015878484.1 16S rRNA pseudouridine(516) synthase RsuA -
  TOLA_RS07145 (Tola_1398) - 1546529..1547140 (-) 612 WP_015878485.1 YigZ family protein -
  TOLA_RS07150 (Tola_1399) - 1547266..1547907 (-) 642 WP_015878486.1 NAD(P)-dependent oxidoreductase -
  TOLA_RS07155 (Tola_1400) - 1548009..1548899 (+) 891 WP_015878487.1 LysR family transcriptional regulator -
  TOLA_RS07160 (Tola_1401) - 1548936..1549394 (+) 459 WP_015878488.1 YccF domain-containing protein -

Sequence

Protein


Download         Length: 379 a.a.        Molecular weight: 42542.05 Da        Isoelectric Point: 5.9816

>NTDB_id=34143 TOLA_RS07130 WP_041609767.1 1543722..1544861(+) (pilU) [Tolumonas auensis DSM 9187]
MEFLDMLIKLAYENGSDLYLSTGAVPSIKFNGVLTPIQQLPMKPGEVAAIADDVMDAEQKVIFEKELEMNLALSLPRVGR
FRVNIFRQRNEISIVARNIKLDIPTFEELKLPPVLLDVIMQKRGLVLFVGGTGSGKSTSLAALIDHRNRNTSGHIITIED
PIEYIHPHKKSIINQREVGVDTRSFHAALKNTLRQAPDVILIGEIRDRETMEHALAFAETGHLAISTLHANNANQALDRI
INFFPEERHAQLMNDLGNNLKAFVSQRLVKTVDGKRHAAVEILLGTATIQDMIRRGDFSNIKEIMEKSVNLGMKTFDQCL
FELYCEGIIDEEEALRNADSLNNLKLKIKLHDENGALEIKGPALNWELDPVKRDEPGFL

Nucleotide


Download         Length: 1140 bp        

>NTDB_id=34143 TOLA_RS07130 WP_041609767.1 1543722..1544861(+) (pilU) [Tolumonas auensis DSM 9187]
ATGGAATTTCTGGATATGCTGATTAAGCTGGCCTATGAAAATGGCTCGGACTTATATCTGTCAACAGGAGCGGTTCCCAG
TATTAAATTTAATGGTGTGCTGACACCAATCCAGCAATTACCGATGAAACCGGGCGAAGTGGCAGCTATTGCCGATGATG
TCATGGATGCGGAACAAAAAGTCATTTTTGAGAAAGAACTGGAAATGAACCTGGCGCTTTCTTTACCCCGGGTTGGCCGT
TTCCGCGTCAATATTTTCCGTCAGCGTAATGAGATCTCGATTGTAGCCCGTAATATCAAACTTGATATTCCTACTTTTGA
AGAGTTAAAGCTGCCACCCGTGTTGCTGGACGTCATCATGCAAAAACGCGGGCTGGTGCTGTTTGTCGGGGGAACAGGCT
CAGGTAAATCAACCTCGTTAGCTGCGCTCATCGATCATCGTAACCGGAATACCAGTGGTCATATCATTACCATTGAAGAT
CCTATCGAATATATTCATCCGCACAAGAAAAGTATCATCAACCAGCGTGAAGTCGGTGTGGATACCCGCAGTTTTCATGC
CGCACTGAAAAATACATTACGTCAGGCTCCGGACGTGATCCTGATCGGGGAAATCCGCGATCGTGAAACCATGGAACATG
CGCTGGCCTTTGCTGAAACCGGTCATTTGGCTATTTCAACGTTGCATGCTAACAATGCGAACCAGGCATTGGATCGTATT
ATTAACTTCTTCCCGGAAGAGCGGCACGCGCAGCTGATGAACGATCTGGGTAATAACCTGAAGGCGTTTGTATCACAACG
ACTGGTAAAAACAGTGGATGGCAAGCGTCATGCTGCGGTCGAGATTTTGTTAGGTACGGCAACCATTCAGGATATGATCC
GCCGTGGTGATTTCTCCAACATCAAAGAAATCATGGAGAAATCGGTCAACCTGGGCATGAAGACGTTTGACCAGTGCCTG
TTTGAGCTTTATTGCGAAGGTATTATTGATGAAGAAGAGGCATTGCGTAATGCAGACTCCCTCAATAACCTGAAACTGAA
GATCAAATTACATGATGAAAATGGCGCTCTGGAAATCAAAGGGCCGGCCTTGAACTGGGAACTTGATCCTGTCAAACGTG
ATGAGCCGGGTTTCCTGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(43-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

53.704

99.736

0.536
  pilU Acinetobacter baylyi ADP1

52.958

93.668

0.496
  pilU Vibrio cholerae strain A1552

47.297

97.625

0.462
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.114

88.127

0.38
  pilT Pseudomonas aeruginosa PAK

41.716

89.182

0.372
  pilT Neisseria gonorrhoeae MS11

40.634

91.557

0.372
  pilT Neisseria meningitidis 8013

40.634

91.557

0.372
  pilT Pseudomonas stutzeri DSM 10701

40.698

90.765

0.369
  pilT Acinetobacter baylyi ADP1

41.515

87.071

0.361

Multiple sequence alignment