Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   TOLA_RS02775 Genome accession   NC_012691
Coordinates   604963..606219 (-) Length   418 a.a.
NCBI ID   WP_012728765.1    Uniprot ID   C4LA37
Organism   Tolumonas auensis DSM 9187     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 599963..611219
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  TOLA_RS02750 (Tola_0532) secA 600002..602725 (+) 2724 WP_012728760.1 preprotein translocase subunit SecA -
  TOLA_RS02755 (Tola_0533) mutT 602794..603192 (+) 399 WP_012728761.1 8-oxo-dGTP diphosphatase MutT -
  TOLA_RS02760 (Tola_0534) yacG 603189..603407 (-) 219 WP_012728762.1 DNA gyrase inhibitor YacG -
  TOLA_RS02765 (Tola_0535) coaE 603456..604073 (-) 618 WP_012728763.1 dephospho-CoA kinase -
  TOLA_RS02770 (Tola_0536) pilD 604073..604948 (-) 876 WP_041609453.1 A24 family peptidase Machinery gene
  TOLA_RS02775 (Tola_0537) pilC 604963..606219 (-) 1257 WP_012728765.1 type II secretion system F family protein Machinery gene
  TOLA_RS02780 (Tola_0538) pilB 606238..607944 (-) 1707 WP_012728766.1 type IV-A pilus assembly ATPase PilB Machinery gene
  TOLA_RS16995 (Tola_0539) - 608024..608467 (-) 444 WP_012728767.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  TOLA_RS02790 (Tola_0540) purD 608764..610050 (-) 1287 WP_012728768.1 phosphoribosylamine--glycine ligase -

Sequence

Protein


Download         Length: 418 a.a.        Molecular weight: 46012.33 Da        Isoelectric Point: 9.7968

>NTDB_id=34133 TOLA_RS02775 WP_012728765.1 604963..606219(-) (pilC) [Tolumonas auensis DSM 9187]
MAVAQQSSAAATANKPKVYHAFVWRGVNRRGQKVSGEMKGETLAQVKADLIRQGVVVQKISKHQESIVTRYTQTIKAMDI
AVLTRQIATMLTAGVPLVQTLRLLASSHEKRPMREMLSTICADIETGTPPSKALRQFPRYFDDLYCDLVASGEQSGALDA
IFDRIAIYREKAEALKSKIKKAMFYPATVIFVALVVSAILLIFVIPEFDKIFKSFGAELPAFTRMVITLSNILQSYWYYV
FGGLVALIFVYVHSYRRMESVRDATDKFILKLPIVGIILQKAALARYARTLSTTFAAGLPLIDGLIAAAGSSGNAVYRHA
IMSVRNEVMSGMQMNVAMRTTNVFPEMVIQMIMIGEESGSLDDMLSKVAGIYEQQVDDAVDALSSLIEPLLMVVLGVLVG
GMVIAMYLPIFKLGSVIK

Nucleotide


Download         Length: 1257 bp        

>NTDB_id=34133 TOLA_RS02775 WP_012728765.1 604963..606219(-) (pilC) [Tolumonas auensis DSM 9187]
ATGGCCGTGGCTCAACAATCCTCCGCCGCTGCAACGGCAAACAAACCTAAGGTCTATCACGCATTTGTCTGGCGCGGTGT
GAACCGCCGTGGTCAGAAAGTGTCCGGTGAGATGAAAGGGGAAACGCTGGCTCAGGTCAAAGCCGATCTGATCCGCCAGG
GAGTCGTAGTTCAGAAAATAAGCAAACATCAGGAATCCATTGTCACCCGCTATACGCAAACCATCAAAGCAATGGATATT
GCGGTATTAACCCGCCAGATTGCCACCATGCTCACTGCCGGGGTACCACTCGTGCAGACACTGCGCTTGCTCGCCAGCAG
CCATGAAAAACGCCCGATGCGGGAAATGCTGTCCACGATCTGTGCGGATATTGAAACCGGCACTCCGCCATCCAAGGCAT
TACGTCAGTTCCCGCGTTATTTTGATGATCTCTATTGTGATCTGGTGGCATCCGGTGAACAGTCCGGCGCGCTGGATGCA
ATATTTGACCGGATTGCCATCTACCGGGAAAAAGCCGAAGCCCTGAAATCCAAAATCAAAAAAGCCATGTTTTATCCGGC
AACCGTTATCTTTGTCGCACTGGTGGTTTCGGCTATTCTTTTGATCTTTGTGATACCTGAATTCGATAAGATTTTCAAAA
GCTTTGGCGCTGAACTCCCCGCGTTTACCCGCATGGTTATTACACTGTCCAATATATTGCAGAGCTACTGGTACTATGTT
TTTGGTGGCCTGGTCGCCCTGATTTTTGTCTATGTTCACTCCTACCGGCGCATGGAGTCCGTCCGTGATGCTACCGATAA
GTTCATCCTGAAACTGCCGATTGTCGGCATTATTTTGCAAAAAGCCGCACTGGCCCGTTATGCCCGGACCCTTTCCACCA
CTTTCGCTGCAGGCCTGCCACTGATTGACGGCCTAATCGCCGCCGCCGGTTCCTCCGGCAATGCGGTATACCGTCACGCC
ATCATGAGTGTCCGTAACGAGGTGATGTCAGGTATGCAGATGAACGTTGCCATGCGCACCACCAACGTGTTTCCGGAAAT
GGTGATCCAGATGATCATGATCGGTGAGGAATCGGGCTCGCTGGATGATATGCTCTCCAAAGTCGCCGGTATCTATGAAC
AGCAGGTGGATGATGCAGTGGATGCCCTCTCCAGCCTGATTGAACCGTTACTCATGGTGGTATTGGGTGTTTTGGTCGGC
GGTATGGTTATCGCCATGTATCTGCCTATCTTTAAACTTGGCAGCGTTATTAAATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(84-206)

Type II secretion system (T2SS), protein F (T2SSF)

(288-409)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB C4LA37

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

52.273

94.737

0.495
  pilC Acinetobacter baumannii D1279779

50.123

96.89

0.486
  pilC Acinetobacter baylyi ADP1

49.261

97.129

0.478
  pilC Legionella pneumophila strain ERS1305867

49.01

96.651

0.474
  pilC Vibrio cholerae strain A1552

47.891

96.411

0.462
  pilC Vibrio campbellii strain DS40M4

47.222

94.737

0.447
  pilG Neisseria meningitidis 44/76-A

40.302

94.976

0.383
  pilG Neisseria gonorrhoeae MS11

40.302

94.976

0.383

Multiple sequence alignment