Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   TOLA_RS02780 Genome accession   NC_012691
Coordinates   606238..607944 (-) Length   568 a.a.
NCBI ID   WP_012728766.1    Uniprot ID   C4LA38
Organism   Tolumonas auensis DSM 9187     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 601238..612944
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  TOLA_RS02755 (Tola_0533) mutT 602794..603192 (+) 399 WP_012728761.1 8-oxo-dGTP diphosphatase MutT -
  TOLA_RS02760 (Tola_0534) yacG 603189..603407 (-) 219 WP_012728762.1 DNA gyrase inhibitor YacG -
  TOLA_RS02765 (Tola_0535) coaE 603456..604073 (-) 618 WP_012728763.1 dephospho-CoA kinase -
  TOLA_RS02770 (Tola_0536) pilD 604073..604948 (-) 876 WP_041609453.1 A24 family peptidase Machinery gene
  TOLA_RS02775 (Tola_0537) pilC 604963..606219 (-) 1257 WP_012728765.1 type II secretion system F family protein Machinery gene
  TOLA_RS02780 (Tola_0538) pilB 606238..607944 (-) 1707 WP_012728766.1 type IV-A pilus assembly ATPase PilB Machinery gene
  TOLA_RS16995 (Tola_0539) - 608024..608467 (-) 444 WP_012728767.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  TOLA_RS02790 (Tola_0540) purD 608764..610050 (-) 1287 WP_012728768.1 phosphoribosylamine--glycine ligase -
  TOLA_RS02795 (Tola_0541) purH 610414..612006 (-) 1593 WP_012728769.1 bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase -
  TOLA_RS02805 (Tola_0543) - 612468..612719 (+) 252 WP_012728771.1 type II toxin-antitoxin system Phd/YefM family antitoxin -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62597.70 Da        Isoelectric Point: 5.1084

>NTDB_id=34134 TOLA_RS02780 WP_012728766.1 606238..607944(-) (pilB) [Tolumonas auensis DSM 9187]
MNASANGLLVSLIRAGLIQDGQSGLLQEKARNEKKTLVTYLIDNNIISSSQLAELCELEYGIPLLDLDAFELSEIPQKYL
NAKLIEKHHALPIYTQGNTLYIALSDPTNITALEDFGFNFNLHTEALLVDERKLQNAIQRILEPTADAFSKSIADDHIAD
FDTGDMDEARLQESLETNSDDDAPIVKYINKVLLDAVRRGASDLHFEPYEKTYRIRFRIDGILYEIATPPVNLANRFSAR
LKVMSRLDIAERRLPQDGRIKLRLAKNRSVDLRVSTLPTMWGEKVVLRILDSSAASLNIDMLGFDDKQKQLYLDTLAKPQ
GMILVTGPTGSGKTVSLYTGLNILNTSELNISTAEDPIEINLAGINQVQVNPKAGLTFANALRSFLRQDPDVVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSAAETLTRLLNMGVPAFNVASSVTLIMAQRLARRLCPHCKKPHHIPEHELLGMGYT
PDEVTAGITLYQPVGCEECSNGYKGRVGIYEMLPMSENIANLIMEGGNSLQIAETAVNEGMVTLYRSGLEKARAGVTSLA
EVNRVTCI

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=34134 TOLA_RS02780 WP_012728766.1 606238..607944(-) (pilB) [Tolumonas auensis DSM 9187]
ATGAATGCTTCCGCCAATGGTTTGTTAGTCAGTCTGATTCGAGCTGGACTCATTCAGGATGGTCAGTCCGGACTATTGCA
GGAAAAAGCCCGTAACGAAAAGAAAACCCTCGTTACTTATCTGATCGATAACAATATTATCAGCAGCAGTCAGCTGGCAG
AGCTCTGTGAGCTGGAGTATGGCATCCCGCTGCTGGATCTGGATGCCTTTGAATTAAGCGAAATCCCGCAGAAATACCTC
AATGCCAAGTTAATCGAAAAACACCACGCCTTACCAATTTATACTCAGGGTAACACCCTCTATATTGCGCTTTCTGATCC
GACCAATATCACCGCGCTGGAAGATTTTGGTTTCAACTTCAATCTGCACACCGAAGCCCTGCTGGTTGATGAACGTAAAC
TGCAAAATGCCATTCAGCGCATTCTCGAACCCACTGCCGATGCATTTTCCAAATCGATAGCGGATGACCATATCGCGGAT
TTTGATACCGGTGATATGGATGAAGCCCGGTTACAGGAATCACTCGAAACCAACAGTGACGACGATGCGCCGATTGTTAA
ATATATTAACAAGGTGCTGCTCGATGCCGTACGCCGCGGCGCCTCTGACTTGCATTTTGAACCGTATGAGAAAACATACC
GCATCCGTTTCCGCATCGACGGTATTTTGTATGAAATCGCCACGCCGCCGGTCAACCTGGCCAACCGCTTTTCTGCCCGC
CTCAAGGTGATGTCACGACTGGATATCGCCGAGCGCCGCTTACCGCAGGATGGCCGTATAAAACTACGCCTGGCGAAAAA
CCGTTCAGTCGATCTGCGAGTCAGTACTTTGCCCACCATGTGGGGCGAAAAAGTGGTATTGCGTATCCTCGATTCTTCTG
CCGCCAGCCTGAATATTGACATGCTCGGTTTTGATGACAAGCAGAAACAGCTTTATCTGGATACGCTGGCGAAACCACAG
GGCATGATCCTGGTCACCGGTCCTACTGGTTCGGGTAAAACCGTATCGCTCTATACCGGCCTGAACATTCTCAACACCAG
TGAACTGAATATCTCCACTGCGGAAGACCCGATAGAAATCAACCTGGCCGGTATTAATCAGGTACAGGTCAATCCCAAAG
CCGGTCTCACCTTTGCCAACGCCCTGCGCTCTTTCCTGCGTCAGGATCCGGATGTCGTGATGGTCGGCGAAATCCGTGAC
CTCGAAACGGCGGAAATCGCCATTAAAGCCGCACAAACTGGTCATCTAGTGCTATCCACCCTGCACACTAACTCCGCAGC
CGAAACCCTGACCCGTTTGCTGAATATGGGCGTACCGGCCTTTAACGTCGCCTCGTCGGTGACCCTAATCATGGCACAGC
GGCTGGCCCGTCGCCTCTGTCCGCATTGTAAAAAGCCGCATCATATCCCGGAGCATGAGCTGTTGGGGATGGGCTATACT
CCGGATGAAGTCACCGCCGGCATCACCCTCTACCAACCCGTGGGTTGCGAGGAATGTTCCAACGGCTATAAAGGCCGTGT
CGGTATTTATGAGATGCTGCCGATGTCTGAAAACATCGCCAATCTGATCATGGAAGGCGGTAACTCATTACAAATCGCCG
AAACGGCGGTCAATGAAGGCATGGTGACGCTCTACCGTTCCGGTCTGGAGAAAGCACGGGCCGGCGTCACCAGTCTGGCC
GAAGTCAACCGCGTGACCTGCATATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(191-458)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(41-143)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB C4LA38

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

55.245

100

0.556
  pilB Acinetobacter baumannii D1279779

57.643

95.599

0.551
  pilB Vibrio cholerae strain A1552

54.594

99.648

0.544
  pilB Legionella pneumophila strain ERS1305867

54.364

96.831

0.526
  pilB Vibrio parahaemolyticus RIMD 2210633

53.047

98.239

0.521
  pilB Vibrio campbellii strain DS40M4

52.867

98.239

0.519
  pilF Neisseria gonorrhoeae MS11

48.399

93.486

0.452
  pilB/pilB1 Synechocystis sp. PCC 6803

40

99.472

0.398
  pilF Thermus thermophilus HB27

38.278

96.127

0.368

Multiple sequence alignment