Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   TOLA_RS02770 Genome accession   NC_012691
Coordinates   604073..604948 (-) Length   291 a.a.
NCBI ID   WP_041609453.1    Uniprot ID   -
Organism   Tolumonas auensis DSM 9187     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 599073..609948
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  TOLA_RS16940 (Tola_0531) - 599747..599878 (-) 132 WP_012728759.1 hypothetical protein -
  TOLA_RS02750 (Tola_0532) secA 600002..602725 (+) 2724 WP_012728760.1 preprotein translocase subunit SecA -
  TOLA_RS02755 (Tola_0533) mutT 602794..603192 (+) 399 WP_012728761.1 8-oxo-dGTP diphosphatase MutT -
  TOLA_RS02760 (Tola_0534) yacG 603189..603407 (-) 219 WP_012728762.1 DNA gyrase inhibitor YacG -
  TOLA_RS02765 (Tola_0535) coaE 603456..604073 (-) 618 WP_012728763.1 dephospho-CoA kinase -
  TOLA_RS02770 (Tola_0536) pilD 604073..604948 (-) 876 WP_041609453.1 A24 family peptidase Machinery gene
  TOLA_RS02775 (Tola_0537) pilC 604963..606219 (-) 1257 WP_012728765.1 type II secretion system F family protein Machinery gene
  TOLA_RS02780 (Tola_0538) pilB 606238..607944 (-) 1707 WP_012728766.1 type IV-A pilus assembly ATPase PilB Machinery gene
  TOLA_RS16995 (Tola_0539) - 608024..608467 (-) 444 WP_012728767.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -

Sequence

Protein


Download         Length: 291 a.a.        Molecular weight: 32784.97 Da        Isoelectric Point: 6.7307

>NTDB_id=34132 TOLA_RS02770 WP_041609453.1 604073..604948(-) (pilD) [Tolumonas auensis DSM 9187]
MDLIFMLYSQYAWLYFFLLGLFSLLVGSFLNVLIHRLPIMLERSWRQEYQDYFAEGDNPPAQTEPYNLFLPRSACPHCGH
QITALENIPLLSWLFLKGRCSSCQHPISARYPLVELLCALASVTVAFYYPPGWALAGALLLTWILLALTFIDFDKLLLPD
QLTLPLLWVGLLLNLSHQFVPLADAVIGAIAGYMVLWSIYWAFKLLTGKEGMGYGDFKLLAALGAWLGWQSLPLILILSS
CVGAILGITLVVMRRHQQSKPMPFGPYLAIAGWIALLWGEQLTNWYLGLLS

Nucleotide


Download         Length: 876 bp        

>NTDB_id=34132 TOLA_RS02770 WP_041609453.1 604073..604948(-) (pilD) [Tolumonas auensis DSM 9187]
ATGGATCTGATTTTTATGCTTTATAGCCAGTACGCCTGGCTATATTTTTTCCTGCTGGGTCTGTTTTCATTGCTGGTTGG
CAGCTTTCTGAATGTACTGATCCATCGTCTGCCCATCATGCTGGAACGCAGCTGGCGACAGGAATATCAGGATTATTTTG
CAGAAGGTGACAATCCCCCGGCACAAACTGAACCTTATAACCTGTTTCTGCCGCGTTCCGCCTGCCCGCATTGCGGCCAT
CAGATCACGGCACTGGAAAATATCCCGTTACTGAGCTGGTTATTTCTCAAAGGCCGCTGCAGTTCATGCCAGCACCCCAT
CAGCGCCCGCTACCCGCTGGTAGAGCTGCTGTGTGCACTGGCGTCCGTCACGGTCGCTTTCTATTATCCGCCCGGCTGGG
CACTGGCCGGCGCACTGCTGCTGACCTGGATTTTGCTGGCACTCACTTTTATCGACTTCGATAAGTTATTGCTGCCGGAT
CAATTAACCCTCCCCCTGCTCTGGGTAGGTTTATTACTCAATCTGTCACACCAGTTTGTACCGCTCGCCGATGCCGTGAT
CGGTGCCATCGCCGGTTATATGGTGTTGTGGTCCATTTACTGGGCGTTCAAACTGCTCACCGGTAAAGAAGGCATGGGCT
ATGGCGATTTCAAATTACTCGCCGCACTCGGTGCCTGGCTGGGCTGGCAATCGCTGCCTCTCATCCTGATCCTCTCCTCC
TGCGTCGGCGCAATACTGGGTATCACACTGGTGGTTATGCGACGTCATCAGCAGAGCAAACCAATGCCGTTCGGCCCCTA
TCTGGCCATTGCCGGCTGGATCGCCTTACTCTGGGGTGAACAGTTAACCAACTGGTATCTGGGCTTGCTCAGCTGA

Domains

Predicted by InterproScan.

Type IV leader peptidase family (Peptidase_A24)

(139-248)

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(21-127)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

59.22

96.907

0.574
  pilD Vibrio campbellii strain DS40M4

53.901

96.907

0.522
  pilD Acinetobacter baumannii D1279779

48.958

98.969

0.485
  pilD Acinetobacter nosocomialis M2

50.916

93.814

0.478
  pilD Neisseria gonorrhoeae MS11

46.014

94.845

0.436

Multiple sequence alignment