Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ERL55_RS12460 Genome accession   NZ_CP035376
Coordinates   2701323..2703044 (+) Length   573 a.a.
NCBI ID   WP_129136698.1    Uniprot ID   -
Organism   Luteimonas sp. YGD11-2     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2696323..2708044
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ERL55_RS12435 pilR 2696911..2698392 (+) 1482 WP_129136693.1 sigma-54 dependent transcriptional regulator Regulator
  ERL55_RS12440 - 2698462..2698746 (+) 285 WP_129136694.1 helix-turn-helix transcriptional regulator -
  ERL55_RS12445 - 2698743..2700029 (+) 1287 WP_129136695.1 HipA domain-containing protein -
  ERL55_RS12450 - 2700032..2700304 (+) 273 WP_129136696.1 hypothetical protein -
  ERL55_RS12455 pilA/pilA1 2700575..2701006 (+) 432 WP_129136697.1 pilin Machinery gene
  ERL55_RS12460 pilB 2701323..2703044 (+) 1722 WP_129136698.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ERL55_RS12465 pilC 2703236..2704507 (+) 1272 WP_129136699.1 type II secretion system F family protein Machinery gene
  ERL55_RS12470 pilD 2704545..2705411 (+) 867 WP_129136700.1 A24 family peptidase Machinery gene
  ERL55_RS12475 - 2705465..2705809 (-) 345 WP_129136701.1 hypothetical protein -
  ERL55_RS12480 xth 2706232..2707098 (+) 867 WP_129136702.1 exodeoxyribonuclease III -
  ERL55_RS12485 - 2707215..2707391 (+) 177 WP_129136703.1 DUF3606 domain-containing protein -

Sequence

Protein


Download         Length: 573 a.a.        Molecular weight: 62593.99 Da        Isoelectric Point: 6.1572

>NTDB_id=339426 ERL55_RS12460 WP_129136698.1 2701323..2703044(+) (pilB) [Luteimonas sp. YGD11-2]
MSAVVSANLVGITGIARRLVMDGALDEATAREALDRASAERKHVSAWLRENRKITGAQLAAANSIEFGMPLFDPAAMNAG
ESAVKLVKDELLRKHCVLPLFKRGGKLFVGTADPTDTRALDEIKFHTNLVVEPILVDEEVLRRTLDLWLEANDSFGDALE
DAEGLESLDVGGDEDGRPDTVADGKDDTPIVKFVNKVLVDAIRKGASDIHFEPYEDDYRVRLRIDGILKQAAKMPVKLNG
RITARLKVMAQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRILDGSAAKLGIEKLGYEPDQQKLFMDA
VHKPYGMVLVTGPTGSGKTVSLYTALGILNDEQRNISTVEDPVEIRLPGVNQVQQNTKRGMTFAAALRSFLRQDPDIIMV
GEIRDLETAEIAVKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCEKCKKPIDLPENALL
AEGFTADEIHAGLKLFDAGSCDDCTNGYKGRTGIYQVMPMNEEIQAIVLAGGNALEIAEASKRSGVRDLRQSALLKVKQG
VTSLAEINRVTKD

Nucleotide


Download         Length: 1722 bp        

>NTDB_id=339426 ERL55_RS12460 WP_129136698.1 2701323..2703044(+) (pilB) [Luteimonas sp. YGD11-2]
ATGAGCGCAGTTGTCTCCGCCAACCTTGTCGGCATCACCGGCATCGCCCGCCGTCTTGTGATGGATGGCGCGCTCGACGA
GGCGACCGCCCGTGAGGCACTCGACCGAGCTTCAGCCGAGCGCAAGCACGTCAGCGCCTGGCTGCGTGAGAATCGCAAGA
TCACCGGCGCCCAGTTGGCAGCCGCGAACTCCATCGAGTTCGGCATGCCGCTGTTCGACCCCGCCGCGATGAATGCGGGC
GAAAGCGCGGTGAAGCTGGTCAAGGACGAGCTGTTGCGCAAGCACTGCGTGCTGCCGCTGTTCAAGCGTGGCGGCAAGCT
CTTCGTGGGCACGGCAGACCCGACCGACACCCGGGCGCTGGACGAAATCAAGTTCCACACCAACCTGGTTGTGGAGCCGA
TCCTGGTGGATGAGGAAGTGCTGCGGCGCACCCTCGACCTGTGGCTCGAGGCCAACGACAGCTTTGGCGACGCGCTCGAA
GATGCGGAAGGACTCGAAAGCCTGGACGTGGGCGGCGACGAGGACGGCCGCCCCGACACCGTGGCCGACGGCAAGGACGA
CACCCCGATCGTCAAGTTCGTCAACAAGGTACTGGTGGATGCGATCCGCAAGGGTGCATCCGACATCCACTTCGAGCCCT
ACGAGGATGATTACCGGGTGCGGCTGCGCATCGACGGCATCCTCAAGCAGGCGGCGAAGATGCCCGTCAAGCTCAACGGG
CGGATCACGGCGCGCCTGAAGGTGATGGCGCAGCTGGACATCGCCGAGAAGCGCGTGCCGCAGGATGGGCGCATCAAATT
GAACCTGTCCAAGACCAAGCAGATCGATTTCCGTGTCAGCACCCTGCCCACCCTGTTCGGCGAGAAGGTGGTGCTGCGTA
TCCTCGACGGCAGCGCGGCGAAGCTGGGCATCGAGAAGCTGGGCTACGAGCCCGACCAGCAGAAGCTGTTCATGGATGCG
GTGCACAAGCCCTACGGCATGGTGCTGGTGACCGGCCCGACCGGCTCGGGCAAGACCGTGTCGCTGTACACGGCGCTGGG
CATCCTCAACGACGAGCAGCGCAATATCTCCACCGTCGAGGATCCGGTGGAAATCCGCCTGCCCGGCGTGAACCAGGTGC
AGCAGAACACCAAGCGCGGCATGACCTTTGCCGCGGCGCTGCGCTCGTTCCTGCGCCAGGACCCGGACATCATCATGGTC
GGCGAAATCCGCGACCTGGAAACCGCCGAGATCGCAGTGAAGGCGGCCCAGACCGGCCATATGGTGCTGTCGACGCTGCA
CACCAACGATGCGCCGCAGACCATTGCGCGCCTGATGAACATGGGGATCGCGCCGTACAACATCACCTCTTCGGTGACGC
TGGTGATCGCACAGCGCCTGGCGCGGCGGCTCTGCGAGAAGTGCAAGAAGCCGATCGACCTGCCGGAGAACGCACTGCTG
GCGGAGGGGTTCACAGCGGACGAGATCCATGCGGGCCTGAAGCTGTTCGACGCCGGCAGCTGCGACGACTGCACCAATGG
CTACAAGGGCCGGACCGGCATCTACCAGGTGATGCCCATGAATGAGGAGATCCAGGCCATCGTGCTGGCGGGCGGCAATG
CGCTGGAGATCGCGGAGGCGTCAAAGCGGTCCGGGGTGCGGGATCTGCGGCAGTCGGCCCTGTTGAAGGTGAAGCAGGGG
GTGACCAGCCTGGCGGAGATCAATCGGGTGACGAAGGACTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(49-146)

Type II/IV secretion system protein (T2SSE)

(196-463)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.417

98.255

0.544
  pilB Acinetobacter baylyi ADP1

54.482

99.302

0.541
  pilB Legionella pneumophila strain ERS1305867

52.12

98.778

0.515
  pilB Vibrio cholerae strain A1552

51.076

89.18

0.455
  pilB Vibrio parahaemolyticus RIMD 2210633

48.485

92.147

0.447
  pilF Neisseria gonorrhoeae MS11

50.707

86.387

0.438
  pilB Vibrio campbellii strain DS40M4

50.615

85.166

0.431
  pilB/pilB1 Synechocystis sp. PCC 6803

35.008

100

0.377
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.245

92.496

0.363
  pilF Thermus thermophilus HB27

39.354

91.798

0.361

Multiple sequence alignment