Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ERL55_RS04030 Genome accession   NZ_CP035376
Coordinates   894153..895283 (+) Length   376 a.a.
NCBI ID   WP_129135286.1    Uniprot ID   -
Organism   Luteimonas sp. YGD11-2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 889153..900283
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ERL55_RS04000 - 889504..890325 (+) 822 WP_129135281.1 cobalamin-binding protein -
  ERL55_RS04005 - 890389..890784 (+) 396 WP_129135282.1 DoxX family protein -
  ERL55_RS04010 - 890804..891277 (-) 474 WP_164972112.1 DUF4426 domain-containing protein -
  ERL55_RS04015 proC 891285..892133 (-) 849 WP_129135284.1 pyrroline-5-carboxylate reductase -
  ERL55_RS04020 - 892151..892861 (-) 711 WP_129135285.1 YggS family pyridoxal phosphate-dependent enzyme -
  ERL55_RS04025 pilT 893028..894065 (+) 1038 WP_100323593.1 type IV pilus twitching motility protein PilT Machinery gene
  ERL55_RS04030 pilU 894153..895283 (+) 1131 WP_129135286.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ERL55_RS04035 - 895293..896249 (-) 957 WP_129135287.1 DUF72 domain-containing protein -
  ERL55_RS04040 - 896425..897045 (+) 621 WP_129135288.1 superoxide dismutase -
  ERL55_RS04045 - 897747..898205 (+) 459 WP_129135289.1 peptidylprolyl isomerase -
  ERL55_RS04050 - 898293..898859 (+) 567 WP_129135290.1 YqgE/AlgH family protein -
  ERL55_RS04055 ruvX 898859..899323 (+) 465 WP_129135291.1 Holliday junction resolvase RuvX -

Sequence

Protein


Download         Length: 376 a.a.        Molecular weight: 41809.09 Da        Isoelectric Point: 6.8810

>NTDB_id=339416 ERL55_RS04030 WP_129135286.1 894153..895283(+) (pilU) [Luteimonas sp. YGD11-2]
MSSIDFTSFLKLMAHQKASDLFITAGMPPSIKVHGKISPVTQNPLTPQQARDLVLNVMTPAQREEFEKTHECNFAIGVSG
VGRFRVSCFYQRNQVGMVLRRIETKIPTVEELNLPPIIKTLAMTKRGIIIFVGATGTGKSTSLAAMIGYRNHNSTGHIIT
IEDPIEFVHKHEGCIITQREVGIDTDSWENALKNTLRQAPDVIMIGEVRTREGMDHAIAFAETGHLVLCTLHANNANQAM
DRIINFFPEDRRSQLLMDLSLNLKGVVAQQLIPTPDGKGRRVAMEVLLGTPLVQDYIRDGEVHKLKDVMKESTNLGMKTF
DQSLFELYQAGEISYEDALRYADSANEVRLRIKLAQGGDARTLSQGLDGVEIAELR

Nucleotide


Download         Length: 1131 bp        

>NTDB_id=339416 ERL55_RS04030 WP_129135286.1 894153..895283(+) (pilU) [Luteimonas sp. YGD11-2]
ATGAGCAGCATCGACTTCACCTCGTTCCTCAAGCTGATGGCCCACCAGAAGGCCTCGGACCTGTTCATCACCGCCGGCAT
GCCGCCGTCAATCAAGGTGCACGGCAAGATCTCGCCGGTGACCCAGAACCCGCTGACGCCGCAGCAGGCGCGCGACCTGG
TGCTCAACGTGATGACCCCCGCGCAGCGCGAGGAATTCGAGAAGACCCACGAGTGCAACTTCGCGATCGGTGTGTCCGGC
GTGGGCCGGTTCCGCGTGTCGTGCTTCTACCAGCGCAACCAGGTGGGCATGGTGCTGCGCCGGATCGAGACCAAGATCCC
GACCGTCGAAGAGCTCAACCTGCCGCCGATCATCAAGACGCTGGCGATGACCAAGCGCGGCATCATCATCTTCGTCGGTG
CCACCGGCACGGGTAAGTCCACGTCGCTGGCGGCGATGATCGGCTACCGCAACCACAACTCGACCGGCCACATCATCACC
ATCGAGGATCCGATCGAGTTCGTGCACAAGCACGAGGGCTGCATCATCACCCAGCGCGAGGTCGGCATCGACACCGACAG
CTGGGAGAACGCGCTGAAGAACACGCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGTACCCGCGAGGGCA
TGGACCACGCCATCGCCTTCGCCGAGACCGGGCATCTCGTGCTGTGCACGCTGCATGCGAACAACGCCAACCAGGCGATG
GACCGCATCATCAACTTCTTCCCCGAGGACCGTCGCAGCCAGCTGCTGATGGACCTCTCGCTCAACCTCAAGGGCGTGGT
CGCGCAACAGCTGATCCCGACGCCCGATGGCAAGGGCCGCCGCGTGGCGATGGAAGTCCTGCTCGGCACCCCGCTGGTGC
AGGACTACATCCGCGACGGCGAGGTCCACAAGCTCAAGGATGTCATGAAGGAATCCACCAACCTCGGCATGAAGACCTTC
GACCAGAGCCTGTTCGAGCTCTACCAGGCCGGGGAGATCTCCTACGAGGACGCGCTGCGCTACGCCGACTCCGCCAACGA
GGTGCGCCTGCGCATCAAGCTCGCCCAGGGCGGCGACGCCCGTACGCTGAGCCAGGGCCTGGACGGGGTGGAGATCGCCG
AGCTCCGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(116-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

68.665

97.606

0.67
  pilU Acinetobacter baylyi ADP1

64.426

94.947

0.612
  pilU Vibrio cholerae strain A1552

54.213

94.681

0.513
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.815

90.691

0.388
  pilT Pseudomonas aeruginosa PAK

40.896

89.096

0.364
  pilT Acinetobacter nosocomialis M2

40.597

89.096

0.362
  pilT Acinetobacter baumannii D1279779

40.597

89.096

0.362
  pilT Acinetobacter baumannii strain A118

40.597

89.096

0.362

Multiple sequence alignment