Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA/cglA/cilD   Type   Machinery gene
Locus tag   EQH17_RS09860 Genome accession   NZ_CP035263
Coordinates   1940771..1941712 (-) Length   313 a.a.
NCBI ID   WP_000249550.1    Uniprot ID   -
Organism   Streptococcus pneumoniae strain TVO_1901922     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 1899825..1953960 1940771..1941712 within 0


Gene organization within MGE regions


Location: 1899825..1953960
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EQH17_RS09550 (EQH17_10110) lytA 1899825..1900781 (-) 957 WP_061632303.1 N-acetylmuramoyl-L-alanine amidase LytA -
  EQH17_RS09555 (EQH17_10115) - 1900785..1901117 (-) 333 WP_061632304.1 phage holin -
  EQH17_RS09560 (EQH17_10120) - 1901121..1901537 (-) 417 WP_001165341.1 phage holin family protein -
  EQH17_RS09565 (EQH17_10125) - 1901547..1901897 (-) 351 WP_000852244.1 hypothetical protein -
  EQH17_RS09570 (EQH17_10130) - 1901900..1902103 (-) 204 WP_001091119.1 hypothetical protein -
  EQH17_RS11115 (EQH17_10135) - 1902084..1902201 (-) 118 Protein_1884 dihydrodipicolinate reductase -
  EQH17_RS09575 (EQH17_10140) - 1902198..1908581 (-) 6384 WP_061632305.1 tail fiber domain-containing protein -
  EQH17_RS09580 (EQH17_10145) - 1908586..1908936 (-) 351 WP_000068031.1 DUF6711 family protein -
  EQH17_RS09585 (EQH17_10150) - 1908945..1912598 (-) 3654 WP_061632306.1 hypothetical protein -
  EQH17_RS09590 (EQH17_10155) - 1912585..1912935 (-) 351 WP_000478010.1 hypothetical protein -
  EQH17_RS09595 (EQH17_10160) - 1912974..1913354 (-) 381 WP_001185635.1 DUF6096 family protein -
  EQH17_RS09600 (EQH17_10165) - 1913359..1913772 (-) 414 WP_000880678.1 phage tail tube protein -
  EQH17_RS09605 (EQH17_10170) - 1913775..1914143 (-) 369 WP_000608233.1 hypothetical protein -
  EQH17_RS09610 (EQH17_10175) - 1914140..1914655 (-) 516 WP_000015941.1 HK97-gp10 family putative phage morphogenesis protein -
  EQH17_RS09615 (EQH17_10180) - 1914630..1914968 (-) 339 WP_000478943.1 hypothetical protein -
  EQH17_RS09620 (EQH17_10185) - 1914949..1915260 (-) 312 WP_000021219.1 phage head-tail connector protein -
  EQH17_RS09625 (EQH17_10190) - 1915262..1915450 (-) 189 WP_000669350.1 hypothetical protein -
  EQH17_RS09630 (EQH17_10195) - 1915440..1915622 (-) 183 WP_000054934.1 Rho termination factor N-terminal domain-containing protein -
  EQH17_RS09635 (EQH17_10200) - 1915634..1916479 (-) 846 WP_000123890.1 N4-gp56 family major capsid protein -
  EQH17_RS09640 (EQH17_10205) - 1916486..1917070 (-) 585 WP_001288024.1 DUF4355 domain-containing protein -
  EQH17_RS09645 (EQH17_10210) - 1917297..1917548 (-) 252 WP_000913247.1 DUF6275 family protein -
  EQH17_RS09650 (EQH17_10215) - 1917550..1917795 (-) 246 WP_000877357.1 hypothetical protein -
  EQH17_RS09655 (EQH17_10220) - 1917847..1918074 (-) 228 WP_050110656.1 hypothetical protein -
  EQH17_RS09660 (EQH17_10225) - 1918062..1919465 (-) 1404 WP_225791266.1 minor capsid protein -
  EQH17_RS09665 (EQH17_10230) - 1919374..1920843 (-) 1470 WP_079106950.1 phage portal protein -
  EQH17_RS09670 (EQH17_10235) - 1920855..1922078 (-) 1224 WP_001864388.1 PBSX family phage terminase large subunit -
  EQH17_RS09675 (EQH17_10240) - 1922068..1922526 (-) 459 WP_061632307.1 hypothetical protein -
  EQH17_RS09680 (EQH17_10245) - 1922555..1923847 (-) 1293 WP_322349249.1 DNA modification methylase -
  EQH17_RS09695 (EQH17_10255) - 1924414..1924836 (-) 423 WP_001030244.1 DUF1492 domain-containing protein -
  EQH17_RS09700 (EQH17_10260) - 1924906..1925271 (-) 366 WP_000802874.1 hypothetical protein -
  EQH17_RS09705 (EQH17_10265) - 1925268..1925588 (-) 321 WP_001268497.1 hypothetical protein -
  EQH17_RS09710 (EQH17_10270) - 1925585..1925998 (-) 414 WP_061632309.1 YopX family protein -
  EQH17_RS09715 (EQH17_10275) - 1925995..1926669 (-) 675 WP_061632310.1 DUF1642 domain-containing protein -
  EQH17_RS09720 (EQH17_10280) - 1926671..1926988 (-) 318 WP_000969680.1 hypothetical protein -
  EQH17_RS09725 (EQH17_10285) - 1927013..1927195 (-) 183 WP_000796349.1 hypothetical protein -
  EQH17_RS09730 (EQH17_10290) - 1927211..1927642 (-) 432 WP_000779143.1 RusA family crossover junction endodeoxyribonuclease -
  EQH17_RS09735 (EQH17_10295) - 1927639..1927968 (-) 330 WP_050105888.1 hypothetical protein -
  EQH17_RS09740 (EQH17_10300) - 1927982..1928191 (-) 210 WP_000455269.1 hypothetical protein -
  EQH17_RS09745 (EQH17_10305) - 1928157..1928663 (-) 507 WP_000034831.1 class I SAM-dependent methyltransferase -
  EQH17_RS09750 (EQH17_10310) ssbA 1928673..1929089 (-) 417 WP_000609561.1 single-stranded DNA-binding protein Machinery gene
  EQH17_RS09755 (EQH17_10315) - 1929184..1929519 (-) 336 WP_000598345.1 sporulation protein Cse60 -
  EQH17_RS09760 (EQH17_10320) - 1929512..1929835 (-) 324 WP_000354630.1 hypothetical protein -
  EQH17_RS09765 (EQH17_10325) - 1929813..1930877 (-) 1065 WP_061632311.1 DUF1351 domain-containing protein -
  EQH17_RS09770 (EQH17_10330) bet 1930887..1931639 (-) 753 WP_050307441.1 phage recombination protein Bet -
  EQH17_RS09775 (EQH17_10335) - 1931657..1931842 (-) 186 WP_000746960.1 hypothetical protein -
  EQH17_RS09780 (EQH17_10340) - 1932096..1932356 (-) 261 WP_000471465.1 hypothetical protein -
  EQH17_RS09785 (EQH17_10345) - 1932349..1932552 (-) 204 WP_061632312.1 hypothetical protein -
  EQH17_RS09790 - 1932552..1932713 (-) 162 WP_179209669.1 BOW99_gp33 family protein -
  EQH17_RS09795 - 1932778..1932921 (-) 144 WP_000389589.1 hypothetical protein -
  EQH17_RS09800 (EQH17_10350) - 1933040..1933231 (+) 192 WP_000834564.1 hypothetical protein -
  EQH17_RS09805 (EQH17_10360) - 1933403..1933570 (-) 168 WP_000152772.1 hypothetical protein -
  EQH17_RS09810 (EQH17_10365) - 1933560..1934414 (-) 855 WP_001862954.1 ATP-binding protein -
  EQH17_RS09815 (EQH17_10370) - 1934424..1935239 (-) 816 WP_050206911.1 replication initiator protein A -
  EQH17_RS09820 (EQH17_10375) - 1935255..1935467 (-) 213 WP_050206913.1 helix-turn-helix transcriptional regulator -
  EQH17_RS09825 (EQH17_10385) - 1935659..1935958 (-) 300 WP_180378152.1 hypothetical protein -
  EQH17_RS09830 (EQH17_10390) - 1936033..1936308 (-) 276 WP_050206914.1 hypothetical protein -
  EQH17_RS09835 (EQH17_10395) - 1936474..1937235 (+) 762 WP_050206915.1 S24 family peptidase -
  EQH17_RS09840 (EQH17_10400) - 1937237..1938001 (+) 765 WP_050073181.1 type II toxin-antitoxin system PemK/MazF family toxin -
  EQH17_RS09845 (EQH17_10405) - 1938280..1939725 (+) 1446 WP_061632313.1 recombinase family protein -
  EQH17_RS09850 (EQH17_10410) - 1939725..1939805 (-) 81 Protein_1938 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  EQH17_RS09855 (EQH17_10415) comGB/cglB 1939807..1940823 (-) 1017 WP_077141332.1 competence type IV pilus assembly protein ComGB Machinery gene
  EQH17_RS09860 (EQH17_10420) comGA/cglA/cilD 1940771..1941712 (-) 942 WP_000249550.1 competence type IV pilus ATPase ComGA Machinery gene
  EQH17_RS09865 (EQH17_10425) - 1941788..1942153 (-) 366 WP_000286415.1 DUF1033 family protein -
  EQH17_RS09870 (EQH17_10430) - 1942304..1943362 (-) 1059 WP_000649468.1 zinc-dependent alcohol dehydrogenase family protein -
  EQH17_RS09875 (EQH17_10435) nagA 1943525..1944676 (-) 1152 WP_001134457.1 N-acetylglucosamine-6-phosphate deacetylase -
  EQH17_RS09880 (EQH17_10440) - 1944829..1946646 (-) 1818 WP_001220850.1 acyltransferase family protein -
  EQH17_RS09885 (EQH17_10445) tgt 1946744..1947886 (-) 1143 WP_001285232.1 tRNA guanosine(34) transglycosylase Tgt -
  EQH17_RS09890 (EQH17_10450) - 1948016..1948873 (+) 858 WP_001108865.1 DUF975 family protein -
  EQH17_RS09895 (EQH17_10460) - 1948900..1949518 (-) 619 Protein_1947 pyroglutamyl-peptidase I -
  EQH17_RS09900 (EQH17_10465) - 1949625..1949993 (-) 369 WP_000022863.1 DUF1304 domain-containing protein -
  EQH17_RS09905 (EQH17_10470) - 1950004..1950428 (-) 425 Protein_1949 MarR family winged helix-turn-helix transcriptional regulator -
  EQH17_RS09910 (EQH17_10475) - 1950748..1951890 (-) 1143 WP_000746983.1 LysM domain-containing protein -
  EQH17_RS09915 (EQH17_10480) - 1952056..1952676 (-) 621 WP_001172823.1 HAD family hydrolase -
  EQH17_RS09920 (EQH17_10485) - 1952680..1953960 (-) 1281 WP_000473959.1 MATE family efflux transporter -

Sequence

Protein


Download         Length: 313 a.a.        Molecular weight: 35516.43 Da        Isoelectric Point: 6.0083

>NTDB_id=338576 EQH17_RS09860 WP_000249550.1 1940771..1941712(-) (comGA/cglA/cilD) [Streptococcus pneumoniae strain TVO_1901922]
MVQEIAQEIIRSARKKGAQDIYFVPKLDAYELHMRVGDERCKIGSYDFEKFAAVISHFKFVAGMNVGEKRRSQLGSCDYA
YDQKIASLRLSTVGDYRGHESLVIRLLHDEEQDLHFWFQDIEELGKQYRQRGLYLFAGPVGSGKTTLMHELSKSLFKGQQ
VMSIEDPVEIKQDDMLQLQLNEAIGLTYENLIKLSLRHRPDLLIIGEIRDSETARAVVRASLTGATVFSTIHAKSIRGVY
ERLLELGVSEEELAVVLQGVCYQRLIGGGGIVDFASRDYQEHQAAKWNEQIDQLLKDGHITSLQAETEKISYS

Nucleotide


Download         Length: 942 bp        

>NTDB_id=338576 EQH17_RS09860 WP_000249550.1 1940771..1941712(-) (comGA/cglA/cilD) [Streptococcus pneumoniae strain TVO_1901922]
ATGGTTCAAGAAATTGCACAAGAAATCATTCGTTCGGCTCGGAAAAAAGGGGCGCAAGACATTTATTTTGTCCCTAAGTT
AGATGCCTATGAGCTTCATATGAGGGTAGGAGACGAGCGCTGTAAAATTGGTAGCTATGATTTTGAAAAGTTTGCAGCCG
TTATCAGTCACTTTAAGTTTGTGGCGGGTATGAATGTGGGAGAAAAAAGACGTAGTCAACTGGGTTCCTGTGATTATGCC
TATGACCAGAAGATAGCGTCTCTACGTTTATCTACTGTAGGCGATTATCGGGGGCATGAGAGTTTGGTTATCCGTTTGTT
GCACGATGAGGAGCAGGACTTGCATTTTTGGTTTCAGGATATTGAAGAATTAGGCAAGCAGTACAGGCAACGGGGACTCT
ATCTTTTTGCTGGTCCGGTTGGGAGTGGTAAGACGACCTTGATGCATGAATTGTCCAAGTCACTCTTTAAAGGACAGCAA
GTTATGTCCATCGAAGATCCTGTCGAAATCAAGCAGGACGACATGCTTCAGTTGCAGTTGAACGAAGCAATCGGCCTAAC
CTATGAAAATCTAATCAAACTTTCCTTGCGTCATCGACCAGATCTCTTGATTATCGGAGAAATTCGTGACAGCGAGACGG
CGCGTGCAGTGGTCAGAGCTAGTTTGACAGGTGCGACAGTCTTTTCAACCATTCACGCCAAAAGTATCCGAGGTGTTTAT
GAGCGTCTGCTGGAGTTGGGTGTGAGTGAAGAAGAATTGGCAGTTGTTCTGCAAGGAGTCTGCTACCAGAGATTAATCGG
GGGAGGAGGAATCGTTGACTTTGCAAGCAGAGATTATCAAGAACACCAAGCAGCCAAGTGGAATGAGCAAATTGACCAGC
TTCTTAAAGATGGACATATCACAAGTCTTCAGGCTGAGACGGAAAAAATTAGCTACAGCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

99.361

100

0.994
  comGA/cglA/cilD Streptococcus pneumoniae D39

99.361

100

0.994
  comGA/cglA/cilD Streptococcus pneumoniae R6

99.361

100

0.994
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

99.361

100

0.994
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

96.486

100

0.965
  comYA Streptococcus gordonii str. Challis substr. CH1

78.387

99.042

0.776
  comYA Streptococcus mutans UA159

66.238

99.361

0.658
  comYA Streptococcus mutans UA140

66.238

99.361

0.658
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

62.903

99.042

0.623
  comGA Lactococcus lactis subsp. cremoris KW2

55.128

99.681

0.55
  comGA Latilactobacillus sakei subsp. sakei 23K

41.912

86.901

0.364

Multiple sequence alignment