Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA/cglA/cilD   Type   Machinery gene
Locus tag   EQH38_RS02140 Genome accession   NZ_CP035242
Coordinates   390177..391118 (+) Length   313 a.a.
NCBI ID   WP_000249548.1    Uniprot ID   -
Organism   Streptococcus pneumoniae strain TVO_1901947     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 385177..396118
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EQH38_RS02120 (EQH38_02235) - 385243..387060 (+) 1818 WP_001220922.1 acyltransferase family protein -
  EQH38_RS02125 (EQH38_02240) nagA 387213..388364 (+) 1152 WP_061367521.1 N-acetylglucosamine-6-phosphate deacetylase -
  EQH38_RS02130 (EQH38_02245) - 388527..389585 (+) 1059 WP_050145166.1 zinc-dependent alcohol dehydrogenase family protein -
  EQH38_RS02135 (EQH38_02250) - 389736..390101 (+) 366 WP_000286415.1 DUF1033 family protein -
  EQH38_RS02140 (EQH38_02255) comGA/cglA/cilD 390177..391118 (+) 942 WP_000249548.1 competence type IV pilus ATPase ComGA Machinery gene
  EQH38_RS02145 (EQH38_02260) comGB/cglB 391066..392082 (+) 1017 WP_074017570.1 competence type IV pilus assembly protein ComGB Machinery gene
  EQH38_RS02150 (EQH38_02265) comGC/cglC 392084..392410 (+) 327 WP_000738629.1 comG operon protein ComGC Machinery gene
  EQH38_RS02155 (EQH38_02270) comGD/cglD 392403..392807 (+) 405 WP_000588013.1 competence type IV pilus minor pilin ComGD Machinery gene
  EQH38_RS02160 (EQH38_02275) comGE 392770..393071 (+) 302 Protein_423 competence type IV pilus minor pilin ComGE -
  EQH38_RS02165 (EQH38_02280) comGF/cglF 393034..393495 (+) 462 WP_000250540.1 competence type IV pilus minor pilin ComGF Machinery gene
  EQH38_RS02170 (EQH38_02285) comGG/cglG 393473..393883 (+) 411 WP_000265621.1 competence type IV pilus minor pilin ComGG Machinery gene
  EQH38_RS02175 - 394020..394607 (+) 588 Protein_426 class I SAM-dependent methyltransferase -
  EQH38_RS02180 (EQH38_02300) comYH 394668..395621 (+) 954 WP_061367493.1 class I SAM-dependent methyltransferase Machinery gene

Sequence

Protein


Download         Length: 313 a.a.        Molecular weight: 35569.54 Da        Isoelectric Point: 6.0083

>NTDB_id=337110 EQH38_RS02140 WP_000249548.1 390177..391118(+) (comGA/cglA/cilD) [Streptococcus pneumoniae strain TVO_1901947]
MVQEIAQEIIRSARKKGAQDIYFVPKLDAYELHMRVGDERCKIGSYDFEKFAAVISHFKFVAGMNVGEKRRSQLGSCDYA
YDQKIASLRLSTVGDYRGHESLVIRLLHDEEQDLHFWFQDIEELGKQYRQRGLYLFAGPVGSGKTTLMHELSKSLFKGQQ
VMSIEDPVEIKQDDMLQLQLNEAIGLTYENLIKLSLRHRPDLLIIGEIRDIETARAVVRASLTGATVFSTIHAKSIRGVY
ERLLELGVSEEELAVVLQGVCYQRLIGGGGIVDFANRDYQEHQAAKWNEQIDQLLKDGHITSLQAETEKISYS

Nucleotide


Download         Length: 942 bp        

>NTDB_id=337110 EQH38_RS02140 WP_000249548.1 390177..391118(+) (comGA/cglA/cilD) [Streptococcus pneumoniae strain TVO_1901947]
ATGGTTCAAGAAATTGCACAAGAAATCATTCGTTCGGCTCGGAAAAAAGGGGCGCAAGACATTTATTTTGTCCCTAAGTT
AGATGCCTATGAGCTTCATATGAGGGTAGGAGACGAGCGCTGTAAAATTGGTAGCTATGATTTTGAAAAGTTTGCAGCCG
TTATCAGTCACTTTAAGTTTGTGGCGGGTATGAATGTGGGAGAAAAAAGACGTAGTCAACTGGGTTCCTGTGATTATGCC
TATGACCAGAAGATAGCGTCTCTACGTTTATCTACTGTAGGCGATTATCGGGGGCATGAGAGTTTGGTTATCCGTTTGTT
GCACGATGAGGAGCAGGACTTGCATTTTTGGTTTCAGGATATTGAAGAATTAGGCAAGCAGTACAGGCAACGGGGACTCT
ATCTTTTTGCTGGCCCGGTTGGGAGTGGTAAGACGACCTTGATGCATGAATTGTCCAAGTCACTCTTTAAAGGACAGCAA
GTTATGTCCATCGAAGATCCTGTCGAAATCAAGCAGGACGACATGCTTCAGTTGCAGTTGAACGAAGCAATCGGCCTAAC
CTATGAAAATCTAATCAAACTTTCCTTGCGTCATCGACCAGATCTCTTGATTATCGGAGAAATTCGTGACATCGAGACGG
CGCGTGCAGTGGTCAGAGCTAGTTTGACAGGTGCGACAGTCTTTTCAACCATTCACGCCAAGAGTATCCGAGGTGTTTAT
GAGCGTCTGCTGGAGTTGGGTGTGAGTGAAGAAGAATTGGCAGTTGTTCTGCAAGGAGTCTGCTACCAGAGATTAATCGG
GGGAGGAGGAATCGTTGACTTTGCAAACAGAGATTATCAAGAACACCAAGCAGCCAAGTGGAATGAGCAAATTGACCAGC
TTCTTAAAGATGGACATATCACAAGTCTTCAGGCTGAGACGGAAAAAATTAGCTACAGCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

98.722

100

0.987
  comGA/cglA/cilD Streptococcus pneumoniae D39

98.722

100

0.987
  comGA/cglA/cilD Streptococcus pneumoniae R6

98.722

100

0.987
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

98.722

100

0.987
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

96.486

100

0.965
  comYA Streptococcus gordonii str. Challis substr. CH1

77.742

99.042

0.77
  comYA Streptococcus mutans UA159

66.238

99.361

0.658
  comYA Streptococcus mutans UA140

66.238

99.361

0.658
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

62.903

99.042

0.623
  comGA Lactococcus lactis subsp. cremoris KW2

55.128

99.681

0.55
  comGA Latilactobacillus sakei subsp. sakei 23K

41.912

86.901

0.364

Multiple sequence alignment