Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB/cglB   Type   Machinery gene
Locus tag   EQH38_RS02145 Genome accession   NZ_CP035242
Coordinates   391066..392082 (+) Length   338 a.a.
NCBI ID   WP_074017570.1    Uniprot ID   -
Organism   Streptococcus pneumoniae strain TVO_1901947     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 386066..397082
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EQH38_RS02125 (EQH38_02240) nagA 387213..388364 (+) 1152 WP_061367521.1 N-acetylglucosamine-6-phosphate deacetylase -
  EQH38_RS02130 (EQH38_02245) - 388527..389585 (+) 1059 WP_050145166.1 zinc-dependent alcohol dehydrogenase family protein -
  EQH38_RS02135 (EQH38_02250) - 389736..390101 (+) 366 WP_000286415.1 DUF1033 family protein -
  EQH38_RS02140 (EQH38_02255) comGA/cglA/cilD 390177..391118 (+) 942 WP_000249548.1 competence type IV pilus ATPase ComGA Machinery gene
  EQH38_RS02145 (EQH38_02260) comGB/cglB 391066..392082 (+) 1017 WP_074017570.1 competence type IV pilus assembly protein ComGB Machinery gene
  EQH38_RS02150 (EQH38_02265) comGC/cglC 392084..392410 (+) 327 WP_000738629.1 comG operon protein ComGC Machinery gene
  EQH38_RS02155 (EQH38_02270) comGD/cglD 392403..392807 (+) 405 WP_000588013.1 competence type IV pilus minor pilin ComGD Machinery gene
  EQH38_RS02160 (EQH38_02275) comGE 392770..393071 (+) 302 Protein_423 competence type IV pilus minor pilin ComGE -
  EQH38_RS02165 (EQH38_02280) comGF/cglF 393034..393495 (+) 462 WP_000250540.1 competence type IV pilus minor pilin ComGF Machinery gene
  EQH38_RS02170 (EQH38_02285) comGG/cglG 393473..393883 (+) 411 WP_000265621.1 competence type IV pilus minor pilin ComGG Machinery gene
  EQH38_RS02175 - 394020..394607 (+) 588 Protein_426 class I SAM-dependent methyltransferase -
  EQH38_RS02180 (EQH38_02300) comYH 394668..395621 (+) 954 WP_061367493.1 class I SAM-dependent methyltransferase Machinery gene
  EQH38_RS02185 (EQH38_02305) - 395672..396862 (+) 1191 WP_000167756.1 acetate kinase -
  EQH38_RS10785 - 396863..396994 (+) 132 WP_000768904.1 hypothetical protein -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 38482.48 Da        Isoelectric Point: 9.6162

>NTDB_id=337111 EQH38_RS02145 WP_074017570.1 391066..392082(+) (comGB/cglB) [Streptococcus pneumoniae strain TVO_1901947]
MDISQVFRLRRKKLATAKQKNIITLFNNLFSSGFHLVETISFLDRSSLLDKQCVTQMRTGLSQGKSFSEMMESLGCSSTI
VTQLSLAEVHGNLHLSLGKIEEYLDNLAKVKKKLIEVATYPLILLGFLLLIMLGLRNYLLPQLDSSNIATRIIGNLPQIF
LGMVGLVSVLALLALTFYKRSSKMSVFSILARLPFIGIFVQTYLTAYYAREWGNMISQGMELTQIFQMMQEQGSQLFKEI
GQDLAQTLKNGREFSQTIGTYPFFRKELSLIIEYGEVKSKLGSELEIYAEKTWEAFFTRVNRTMNLVQPLVFIFVALIIV
LLYAAMLMPMYQNMEVNF

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=337111 EQH38_RS02145 WP_074017570.1 391066..392082(+) (comGB/cglB) [Streptococcus pneumoniae strain TVO_1901947]
ATGGACATATCACAAGTCTTCAGGCTGAGACGGAAAAAATTAGCTACAGCTAAGCAAAAAAATATCATCACCCTATTTAA
CAATCTCTTTTCTAGCGGTTTTCATCTGGTGGAGACTATCTCCTTTTTAGATAGGAGTTCCTTGTTGGACAAGCAGTGTG
TGACCCAGATGCGTACAGGCTTGTCTCAGGGGAAATCATTCTCAGAAATGATGGAAAGTTTGGGATGTTCAAGTACCATT
GTCACTCAGTTATCTCTAGCTGAAGTTCATGGCAATCTTCACCTGAGTTTGGGAAAGATAGAAGAATATCTAGACAATCT
GGCCAAGGTCAAGAAAAAGTTAATTGAAGTAGCGACCTATCCTTTGATTTTGCTGGGTTTTCTTCTCTTAATTATGCTGG
GGCTACGGAATTACCTGCTCCCACAACTGGATAGTAGCAATATTGCCACCCGAATTATCGGTAATCTGCCACAAATTTTT
CTAGGCATGGTAGGGCTTGTTTCCGTGCTTGCCCTTTTAGCACTAACTTTTTATAAAAGAAGTTCTAAGATGAGTGTCTT
TTCTATCTTAGCACGCCTTCCCTTTATTGGAATCTTTGTGCAGACCTACTTGACAGCCTATTATGCACGTGAATGGGGGA
ATATGATTTCACAGGGAATGGAGCTGACGCAGATTTTTCAAATGATGCAGGAACAAGGTTCCCAGCTCTTTAAAGAAATC
GGTCAAGATCTGGCTCAAACCCTGAAAAATGGCCGTGAATTTTCTCAGACGATAGGAACCTATCCGTTCTTTAGGAAGGA
ATTGAGTCTCATCATAGAGTATGGGGAAGTTAAGTCCAAGCTGGGTAGTGAGTTGGAAATCTATGCTGAAAAAACTTGGG
AAGCCTTTTTTACCCGAGTCAACCGCACCATGAATTTGGTGCAGCCACTGGTTTTTATCTTTGTGGCACTGATTATCGTT
TTACTTTATGCGGCAATGCTCATGCCCATGTATCAAAATATGGAGGTAAATTTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(24-141)

Type II secretion system (T2SS), protein F (T2SSF)

(209-329)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB/cglB Streptococcus pneumoniae Rx1

98.521

100

0.985
  comGB/cglB Streptococcus pneumoniae D39

98.521

100

0.985
  comGB/cglB Streptococcus pneumoniae R6

98.521

100

0.985
  comGB/cglB Streptococcus pneumoniae TIGR4

98.521

100

0.985
  comGB/cglB Streptococcus mitis SK321

94.97

100

0.95
  comGB/cglB Streptococcus mitis NCTC 12261

94.083

100

0.941
  comYB Streptococcus gordonii str. Challis substr. CH1

70.536

99.408

0.701
  comYB Streptococcus mutans UA140

58.176

94.083

0.547
  comYB Streptococcus mutans UA159

58.176

94.083

0.547
  comGB Lactococcus lactis subsp. cremoris KW2

50.898

98.817

0.503

Multiple sequence alignment