Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF/cglF   Type   Machinery gene
Locus tag   SP70585_RS10610 Genome accession   NC_012468
Coordinates   1957691..1958152 (-) Length   153 a.a.
NCBI ID   WP_000250520.1    Uniprot ID   C1CA95
Organism   Streptococcus pneumoniae 70585     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1952691..1963152
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SP70585_RS10585 (SP70585_2131) comYH 1953917..1954870 (-) 954 WP_000345132.1 class I SAM-dependent methyltransferase Machinery gene
  SP70585_RS12905 - 1955216..1956018 (+) 803 Protein_1995 IS5 family transposase -
  SP70585_RS10600 (SP70585_2134) - 1956169..1956645 (+) 477 WP_000777686.1 GNAT family N-acetyltransferase -
  SP70585_RS10605 (SP70585_2135) comGG/cglG 1957300..1957713 (-) 414 WP_000265615.1 competence type IV pilus minor pilin ComGG Machinery gene
  SP70585_RS10610 (SP70585_2136) comGF/cglF 1957691..1958152 (-) 462 WP_000250520.1 competence type IV pilus minor pilin ComGF Machinery gene
  SP70585_RS10615 (SP70585_2137) comGE/cglE 1958115..1958417 (-) 303 WP_000413382.1 competence type IV pilus minor pilin ComGE Machinery gene
  SP70585_RS10620 (SP70585_2138) comGD/cglD 1958380..1958814 (-) 435 WP_033606693.1 competence type IV pilus minor pilin ComGD Machinery gene
  SP70585_RS10625 (SP70585_2139) comGC/cglC 1958777..1959100 (-) 324 WP_000738628.1 comG operon protein ComGC Machinery gene
  SP70585_RS10630 (SP70585_2140) comGB/cglB 1959102..1960118 (-) 1017 WP_078141518.1 competence type IV pilus assembly protein ComGB Machinery gene
  SP70585_RS10635 (SP70585_2141) comGA/cglA/cilD 1960066..1961007 (-) 942 WP_000249572.1 competence type IV pilus ATPase ComGA Machinery gene
  SP70585_RS10640 (SP70585_2142) - 1961083..1961448 (-) 366 WP_000286415.1 DUF1033 family protein -
  SP70585_RS10645 (SP70585_2143) - 1961599..1962657 (-) 1059 WP_000649465.1 zinc-dependent alcohol dehydrogenase family protein -

Sequence

Protein


Download         Length: 153 a.a.        Molecular weight: 17905.50 Da        Isoelectric Point: 8.0031

>NTDB_id=33339 SP70585_RS10610 WP_000250520.1 1957691..1958152(-) (comGF/cglF) [Streptococcus pneumoniae 70585]
MVQNSCWQSKSHKVKAFTLLESLIALIVISGGLLLFQAMSQLLISEVRYQQQSEQKEWLLFVDQLEAELDRSQFEKVEGN
RLYMKQDGKEIAMGKSKADDFRKTNARGRGYQPMVYGLKSAQITEDNQLVHFRFQFQKGLEREFIYRVEKEES

Nucleotide


Download         Length: 462 bp        

>NTDB_id=33339 SP70585_RS10610 WP_000250520.1 1957691..1958152(-) (comGF/cglF) [Streptococcus pneumoniae 70585]
ATGGTTCAGAACAGTTGTTGGCAATCAAAGAGCCATAAGGTCAAGGCTTTTACCTTGTTAGAATCCCTGATTGCCCTGAT
TGTCATCAGTGGGGGATTACTCCTTTTTCAAGCTATGAGTCAGCTCCTCATTTCAGAAGTTCGCTACCAGCAACAAAGCG
AGCAAAAGGAGTGGCTCTTGTTTGTGGACCAACTTGAGGCAGAATTAGACCGTTCGCAGTTCGAAAAAGTAGAAGGCAAT
CGCCTATACATGAAGCAAGATGGCAAGGAGATTGCTATGGGTAAGTCTAAGGCAGATGATTTCCGTAAAACGAATGCTCG
TGGTCGAGGTTATCAGCCTATGGTTTATGGCCTCAAATCAGCTCAGATTACAGAGGACAATCAACTGGTTCATTTTCGTT
TCCAGTTTCAAAAAGGCTTAGAAAGGGAGTTCATCTATCGTGTGGAAAAAGAAGAAAGTTAA

Domains

Predicted by InterproScan.

Putative Competence protein ComGF (ComGF)

(45-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB C1CA95

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF/cglF Streptococcus pneumoniae Rx1

93.464

100

0.935
  comGF/cglF Streptococcus pneumoniae D39

93.464

100

0.935
  comGF/cglF Streptococcus pneumoniae R6

93.464

100

0.935
  comGF/cglF Streptococcus pneumoniae TIGR4

93.464

100

0.935
  comGF/cglF Streptococcus mitis NCTC 12261

90.85

100

0.909
  comGF/cglF Streptococcus mitis SK321

90.85

100

0.909

Multiple sequence alignment