Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   EMK97_RS14230 Genome accession   NZ_CP034759
Coordinates   3194046..3195293 (-) Length   415 a.a.
NCBI ID   WP_130603286.1    Uniprot ID   A0A4P6P546
Organism   Litorilituus sediminis strain JCM 17549     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3189046..3200293
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EMK97_RS14205 (EMK97_14205) mutT 3190859..3191281 (+) 423 WP_130603276.1 8-oxo-dGTP diphosphatase MutT -
  EMK97_RS14210 (EMK97_14210) yacG 3191289..3191525 (-) 237 WP_130603278.1 DNA gyrase inhibitor YacG -
  EMK97_RS14215 (EMK97_14215) zapD 3191585..3192331 (-) 747 WP_130603280.1 cell division protein ZapD -
  EMK97_RS14220 (EMK97_14220) coaE 3192442..3193050 (-) 609 WP_130603282.1 dephospho-CoA kinase -
  EMK97_RS14225 (EMK97_14225) pilD 3193063..3193965 (-) 903 WP_130603284.1 A24 family peptidase Machinery gene
  EMK97_RS14230 (EMK97_14230) pilC 3194046..3195293 (-) 1248 WP_130603286.1 type II secretion system F family protein Machinery gene
  EMK97_RS14235 (EMK97_14235) pilB 3195422..3197122 (-) 1701 WP_130603288.1 type IV-A pilus assembly ATPase PilB Machinery gene
  EMK97_RS19370 (EMK97_14240) - 3197341..3197826 (-) 486 WP_211342248.1 pilin -
  EMK97_RS14245 (EMK97_14245) - 3198059..3199918 (-) 1860 WP_130603292.1 sulfatase-like hydrolase/transferase -

Sequence

Protein


Download         Length: 415 a.a.        Molecular weight: 44928.94 Da        Isoelectric Point: 9.4310

>NTDB_id=332890 EMK97_RS14230 WP_130603286.1 3194046..3195293(-) (pilC) [Litorilituus sediminis strain JCM 17549]
MAVSAKKAANKPKELDVFIWNGVNRKGKKINGELPANNIIELKAQLRKQGITPSRVKKKPKPLFGIGGGDKAIKPADIAV
ITRQIATMLGAGVPLVQSIEMIGKGHDNGNMRKLLAEIGTKLASGIPLSDCLREHPKYFDDLYCDLVASGEQSGALETIY
GRIATYKEKAEALKAKIKKAMTYPISVLVIAAIVTSILLIFVVPVFQDIFNGFGAELPAFTLLVIAISEFMQAYWYMGLG
AIFAFIYLFKRVHRSNQKFRDNVDKAILKLPVFGDLLEKAAVARYARTLSTTFAAGVPLIDALESAAGASGNAVFRDAIL
EIRNEVASGMQMNLAMRNSSIFPDMVIQMVAIGEESGAVDDMLSKVANVYEAEVDNAVDNLTALLEPMIMAFLGVVIGGL
IIAMYLPIFQIGSIV

Nucleotide


Download         Length: 1248 bp        

>NTDB_id=332890 EMK97_RS14230 WP_130603286.1 3194046..3195293(-) (pilC) [Litorilituus sediminis strain JCM 17549]
ATGGCAGTATCAGCAAAAAAAGCCGCAAATAAACCCAAAGAACTCGATGTTTTTATCTGGAATGGCGTAAATCGCAAAGG
TAAGAAAATAAATGGTGAGCTTCCTGCCAATAATATTATCGAACTAAAAGCCCAGCTTAGAAAACAAGGAATCACGCCAA
GTAGGGTAAAGAAAAAGCCTAAGCCTTTATTTGGCATTGGCGGTGGCGACAAAGCTATAAAGCCTGCCGATATTGCTGTT
ATTACCAGACAAATAGCTACCATGTTGGGTGCGGGTGTACCATTAGTGCAAAGCATTGAAATGATAGGCAAGGGACATGA
TAACGGTAATATGCGCAAACTTTTGGCAGAAATTGGTACTAAATTGGCCTCCGGTATACCACTATCTGACTGTTTAAGAG
AACATCCTAAATACTTTGATGATTTGTATTGTGACTTAGTCGCCTCTGGTGAACAATCTGGTGCTTTAGAAACCATCTAC
GGCCGAATTGCTACGTACAAAGAAAAAGCAGAAGCGTTAAAAGCAAAAATTAAGAAAGCCATGACCTATCCTATCTCAGT
CTTAGTCATTGCTGCCATCGTCACCTCAATTTTACTTATTTTTGTAGTGCCTGTTTTCCAAGATATTTTCAATGGATTTG
GTGCTGAACTACCTGCATTTACCTTATTAGTTATTGCCATATCAGAATTTATGCAGGCGTATTGGTATATGGGTCTAGGG
GCTATATTTGCCTTTATATATCTATTTAAGCGAGTTCATCGAAGCAATCAAAAATTTAGAGATAACGTTGATAAAGCTAT
CTTGAAATTGCCTGTTTTTGGCGATTTATTGGAAAAAGCTGCTGTTGCTCGCTATGCACGAACTTTATCAACTACCTTTG
CTGCCGGGGTACCGCTGATAGATGCTTTGGAATCTGCCGCAGGAGCCTCTGGCAATGCCGTTTTTCGTGATGCCATTTTA
GAAATTCGTAATGAGGTAGCCTCAGGTATGCAAATGAACTTAGCGATGCGTAACTCTAGCATTTTCCCTGATATGGTTAT
TCAAATGGTAGCAATAGGTGAGGAGTCAGGTGCAGTAGATGATATGCTATCTAAGGTAGCTAATGTGTATGAAGCAGAAG
TAGACAATGCTGTTGATAACTTAACCGCATTATTAGAGCCTATGATTATGGCTTTCCTTGGTGTAGTAATTGGTGGGCTT
ATCATTGCAATGTACTTACCTATCTTCCAAATTGGCTCTATTGTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(286-407)

Type II secretion system (T2SS), protein F (T2SSF)

(82-204)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A4P6P546

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

61.425

98.072

0.602
  pilC Acinetobacter baumannii D1279779

55.392

98.313

0.545
  pilC Acinetobacter baylyi ADP1

54.902

98.313

0.54
  pilC Legionella pneumophila strain ERS1305867

52.709

97.831

0.516
  pilC Vibrio cholerae strain A1552

44.472

98.072

0.436
  pilG Neisseria meningitidis 44/76-A

44.03

96.867

0.427
  pilG Neisseria gonorrhoeae MS11

43.781

96.867

0.424
  pilC Vibrio campbellii strain DS40M4

42.611

97.831

0.417

Multiple sequence alignment