Detailed information NTG0332889

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: EMK97_RS14225
• Genome accession: NZ_CP034759
• Coordinates: 3193063..3193965 (-)
• Length: 300 a.a.
• NCBI ID: WP_130603284.1
• Uniprot ID: A0A4P6PAV8
• Organism: Litorilituus sediminis strain JCM 17549
• Function: type IV pilus biogenesis and function (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3188063..3198965

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
EMK97_RS14200 (EMK97_14200)	secA	3188079..3190778 (+)	2700	WP_130603274.1	preprotein translocase subunit SecA	-
EMK97_RS14205 (EMK97_14205)	mutT	3190859..3191281 (+)	423	WP_130603276.1	8-oxo-dGTP diphosphatase MutT	-
EMK97_RS14210 (EMK97_14210)	yacG	3191289..3191525 (-)	237	WP_130603278.1	DNA gyrase inhibitor YacG	-
EMK97_RS14215 (EMK97_14215)	zapD	3191585..3192331 (-)	747	WP_130603280.1	cell division protein ZapD	-
EMK97_RS14220 (EMK97_14220)	coaE	3192442..3193050 (-)	609	WP_130603282.1	dephospho-CoA kinase	-
EMK97_RS14225 (EMK97_14225)	pilD	3193063..3193965 (-)	903	WP_130603284.1	A24 family peptidase	Machinery gene
EMK97_RS14230 (EMK97_14230)	pilC	3194046..3195293 (-)	1248	WP_130603286.1	type II secretion system F family protein	Machinery gene
EMK97_RS14235 (EMK97_14235)	pilB	3195422..3197122 (-)	1701	WP_130603288.1	type IV-A pilus assembly ATPase PilB	Machinery gene
EMK97_RS19370 (EMK97_14240)	-	3197341..3197826 (-)	486	WP_211342248.1	pilin	-

Sequence

Protein

• Length: 300 a.a.
• Molecular weight: 34114.45 Da
• Isoelectric Point: 6.3236

>NTDB_id=332889 EMK97_RS14225 WP_130603284.1 3193063..3193965(-) (pilD) [Litorilituus sediminis strain JCM 17549]
MFNFSFLNDITELFQQSPAYFYVSIALLSLCIGSFLNVVIYRLPKMMEYTWYHECRDFLYDEVKDIKPKEMQEVTLSKPD
STCPSCGHKIRYYENIPVLSWLFLRGKCSQCKTKISARYPIIEATTTVLSLVIAQHFGVSAQTFWLLILTWCLIALTMID
FDHMLLPDQITLPLLWLGLLLNINETFVPLQDAVIGAAAGYMSLFSVFWLFKLLTGKEGMGFGDFKLLAVFGAWAGWQLL
PLLILMASVVGAIIGIALMVFNNHKKEQAIPFGPYIAIAGWITILWGESIWSWYLLQLGG

Nucleotide

• Length: 903 bp

>NTDB_id=332889 EMK97_RS14225 WP_130603284.1 3193063..3193965(-) (pilD) [Litorilituus sediminis strain JCM 17549]
GTGTTTAATTTCTCGTTTTTAAATGACATTACTGAACTCTTTCAACAGTCACCCGCCTATTTTTATGTTTCAATTGCCTT
ACTTTCCCTTTGCATCGGTAGTTTCTTAAACGTGGTTATCTATCGTTTACCGAAAATGATGGAATATACCTGGTACCATG
AATGCCGCGACTTTTTATATGATGAAGTTAAAGATATTAAGCCTAAAGAAATGCAAGAAGTCACCCTCTCTAAGCCAGAT
TCAACTTGCCCAAGCTGTGGACATAAAATCAGATATTACGAAAACATTCCTGTATTAAGCTGGCTATTTTTAAGAGGTAA
ATGTAGCCAATGTAAGACTAAGATTTCAGCGCGCTACCCCATAATAGAAGCAACAACCACTGTTTTAAGCCTTGTTATCG
CTCAACATTTTGGTGTTAGTGCACAAACATTTTGGTTGCTGATTTTAACTTGGTGTTTAATTGCGCTTACTATGATTGAT
TTTGATCATATGTTGCTACCTGATCAAATCACCTTACCGCTATTATGGCTTGGTCTATTACTAAACATTAATGAAACCTT
TGTCCCCTTGCAAGATGCAGTAATTGGTGCCGCAGCTGGTTATATGAGTTTATTTAGCGTTTTTTGGCTATTTAAATTAC
TGACAGGCAAAGAAGGTATGGGCTTTGGTGATTTTAAACTTCTTGCTGTATTTGGTGCTTGGGCAGGCTGGCAATTACTG
CCTTTACTTATTTTAATGGCCTCAGTTGTCGGCGCTATAATAGGTATCGCGCTTATGGTTTTCAACAATCATAAAAAAGA
GCAAGCAATTCCTTTTGGTCCTTATATAGCCATTGCTGGGTGGATAACCATTCTTTGGGGTGAAAGCATTTGGTCTTGGT
ATTTGCTCCAACTTGGCGGCTAA

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (27-137)

• Type IV leader peptidase family (Peptidase_A24) (147-256)

3D structure

• AlphaFold DB: A0A4P6PAV8

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio campbellii strain DS40M4	54.483	96.667	0.527
pilD	Vibrio cholerae strain A1552	55.634	94.667	0.527
pilD	Acinetobacter baumannii D1279779	52.249	96.333	0.503
pilD	Acinetobacter nosocomialis M2	51.903	96.333	0.5
pilD	Neisseria gonorrhoeae MS11	48.718	91	0.443