Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   EK374_RS17895 Genome accession   NZ_CP034683
Coordinates   3960671..3961891 (+) Length   406 a.a.
NCBI ID   WP_127025895.1    Uniprot ID   -
Organism   Rheinheimera mangrovi strain LHK132     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3955671..3966891
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EK374_RS17870 - 3956090..3956608 (+) 519 WP_127025890.1 TIGR02281 family clan AA aspartic protease -
  EK374_RS17875 nadC 3956657..3957526 (+) 870 WP_127025891.1 carboxylating nicotinate-nucleotide diphosphorylase -
  EK374_RS17880 - 3957643..3958296 (+) 654 WP_127025892.1 DUF1294 domain-containing protein -
  EK374_RS17885 - 3958466..3958849 (+) 384 WP_127025893.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  EK374_RS17890 pilB 3958943..3960640 (+) 1698 WP_127025894.1 type IV-A pilus assembly ATPase PilB Machinery gene
  EK374_RS17895 pilC 3960671..3961891 (+) 1221 WP_127025895.1 type II secretion system F family protein Machinery gene
  EK374_RS17900 pilD 3961891..3962772 (+) 882 WP_127025896.1 A24 family peptidase Machinery gene
  EK374_RS17905 coaE 3962794..3963417 (+) 624 WP_127025897.1 dephospho-CoA kinase -
  EK374_RS17910 zapD 3963456..3964202 (+) 747 WP_127025898.1 cell division protein ZapD -
  EK374_RS17915 yacG 3964320..3964550 (+) 231 WP_127025899.1 DNA gyrase inhibitor YacG -
  EK374_RS17920 mutT 3964618..3965022 (-) 405 WP_127025900.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 406 a.a.        Molecular weight: 43728.34 Da        Isoelectric Point: 9.2637

>NTDB_id=332464 EK374_RS17895 WP_127025895.1 3960671..3961891(+) (pilC) [Rheinheimera mangrovi strain LHK132]
MAEVKLKQLDIFLWKGINRRGKTSNGEIRAGSVAEAKALLRQQGFTPSSVKKKSKPMAFTQPSIKSSDISVVTRQIATML
GAGVPLVQSIDLIASGASNETLRQLMAKISIKVQGGTPLSEVLREHKDHFDELYCDLVKSGEQSGALDRIFDRIAIYKEK
AEALKSKIKKAMFYPIAVVVVAGIVTSILLIFVVPQFAEIFGNMGAELPAFTLFVLGISEFMQAYWYYALGAIIAAVFAM
SKSYKNSQSFKVAADHAILRLPIIGMILNKAAVARYARTLSTTFAAGVPLIEALESAAGAAGNEKYRAAILSVREEVAAG
NQMHLAMKNTASFPEMVNQMVAIGEESGALDSMLSKVATIYEQEVDDAVDGLTALLEPLIMAVLGVLIGGLIIAMYLPIF
QLGSIV

Nucleotide


Download         Length: 1221 bp        

>NTDB_id=332464 EK374_RS17895 WP_127025895.1 3960671..3961891(+) (pilC) [Rheinheimera mangrovi strain LHK132]
ATGGCCGAAGTAAAACTTAAGCAGCTGGATATCTTCCTGTGGAAAGGCATAAACAGGAGGGGTAAAACCAGCAATGGTGA
GATCAGGGCAGGCTCAGTCGCGGAAGCCAAGGCTTTGCTGCGTCAACAAGGCTTTACTCCTTCTTCAGTGAAGAAAAAAA
GCAAACCCATGGCCTTTACTCAGCCTTCGATTAAGTCGTCCGATATTTCCGTCGTCACCCGACAAATAGCCACTATGCTG
GGCGCTGGCGTGCCTTTGGTACAAAGCATTGATCTGATTGCCAGCGGTGCCAGTAACGAAACCTTACGCCAGTTGATGGC
TAAAATATCAATCAAAGTACAAGGCGGAACGCCTTTATCCGAAGTCCTGAGGGAACACAAAGATCATTTTGATGAGTTGT
ACTGCGATTTGGTGAAGTCCGGTGAACAGTCCGGCGCTTTAGACAGAATTTTTGACCGCATAGCCATTTACAAAGAAAAA
GCCGAAGCACTGAAATCTAAAATTAAAAAAGCCATGTTTTATCCTATAGCTGTGGTTGTAGTCGCAGGTATAGTGACCTC
TATCTTATTAATTTTTGTGGTACCACAGTTCGCTGAAATTTTTGGCAATATGGGCGCAGAGCTGCCAGCTTTTACCCTGT
TTGTGCTGGGCATTTCGGAGTTTATGCAAGCCTACTGGTATTACGCTCTTGGCGCCATCATCGCCGCCGTATTTGCTATG
TCTAAATCTTATAAAAACAGCCAGAGCTTTAAAGTAGCAGCCGATCATGCAATTTTAAGACTACCCATTATAGGTATGAT
TTTAAATAAAGCTGCTGTAGCACGCTACGCCCGCACTTTATCCACCACCTTTGCCGCTGGTGTGCCTTTGATTGAAGCTT
TAGAATCTGCAGCCGGAGCGGCAGGCAACGAGAAATACCGGGCTGCCATTTTGTCAGTGCGTGAAGAAGTAGCAGCAGGT
AATCAAATGCATCTGGCAATGAAAAATACCGCATCTTTTCCTGAGATGGTCAATCAAATGGTAGCAATAGGCGAAGAGTC
AGGTGCGCTGGACAGTATGCTGTCAAAAGTCGCCACTATCTATGAACAGGAAGTGGACGATGCTGTAGATGGTTTAACTG
CCTTACTTGAGCCCTTGATTATGGCGGTGTTAGGTGTCTTAATAGGCGGACTTATTATTGCGATGTATCTTCCGATCTTC
CAACTGGGCAGTATTGTGTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(277-398)

Type II secretion system (T2SS), protein F (T2SSF)

(73-195)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

55.172

100

0.552
  pilC Legionella pneumophila strain ERS1305867

54.408

97.783

0.532
  pilC Acinetobacter baumannii D1279779

52.206

100

0.525
  pilC Acinetobacter baylyi ADP1

50.245

100

0.505
  pilC Vibrio cholerae strain A1552

46.366

98.276

0.456
  pilC Vibrio campbellii strain DS40M4

42.786

99.015

0.424
  pilG Neisseria meningitidis 44/76-A

42.784

95.567

0.409
  pilG Neisseria gonorrhoeae MS11

42.526

95.567

0.406
  pilC Thermus thermophilus HB27

38.462

99.261

0.382

Multiple sequence alignment