Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   EK374_RS17890 Genome accession   NZ_CP034683
Coordinates   3958943..3960640 (+) Length   565 a.a.
NCBI ID   WP_127025894.1    Uniprot ID   -
Organism   Rheinheimera mangrovi strain LHK132     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3953943..3965640
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EK374_RS17860 ampE 3954576..3955463 (-) 888 WP_127025888.1 beta-lactamase regulator AmpE -
  EK374_RS17865 ampD 3955464..3956012 (-) 549 WP_233280278.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  EK374_RS17870 - 3956090..3956608 (+) 519 WP_127025890.1 TIGR02281 family clan AA aspartic protease -
  EK374_RS17875 nadC 3956657..3957526 (+) 870 WP_127025891.1 carboxylating nicotinate-nucleotide diphosphorylase -
  EK374_RS17880 - 3957643..3958296 (+) 654 WP_127025892.1 DUF1294 domain-containing protein -
  EK374_RS17885 - 3958466..3958849 (+) 384 WP_127025893.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  EK374_RS17890 pilB 3958943..3960640 (+) 1698 WP_127025894.1 type IV-A pilus assembly ATPase PilB Machinery gene
  EK374_RS17895 pilC 3960671..3961891 (+) 1221 WP_127025895.1 type II secretion system F family protein Machinery gene
  EK374_RS17900 pilD 3961891..3962772 (+) 882 WP_127025896.1 A24 family peptidase Machinery gene
  EK374_RS17905 coaE 3962794..3963417 (+) 624 WP_127025897.1 dephospho-CoA kinase -
  EK374_RS17910 zapD 3963456..3964202 (+) 747 WP_127025898.1 cell division protein ZapD -
  EK374_RS17915 yacG 3964320..3964550 (+) 231 WP_127025899.1 DNA gyrase inhibitor YacG -
  EK374_RS17920 mutT 3964618..3965022 (-) 405 WP_127025900.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 565 a.a.        Molecular weight: 62115.57 Da        Isoelectric Point: 6.6705

>NTDB_id=332463 EK374_RS17890 WP_127025894.1 3958943..3960640(+) (pilB) [Rheinheimera mangrovi strain LHK132]
MALNPSSILLKRLHQANLVDPQITAKLLHEKSHLYQTIADLLIGEKLIQSKVLADAAASFTLHSSMDLDFFDINAIPEQL
RNEKLIRKHHILPLSKRHKTLFLAVVDPTDMTSVEDFEFNTGLNAEMIVVEFDKLHKAIDKLFETTATDSFTDTQWDLAK
MGLAGGEEEDTKTVGSDSEDQPIISFINKMLHDAIRKGASDLHFEPYEKSYRIRFRIDGILHEVASPPVALSTRLSARLK
VMSRLDIAEKRVPQDGRIKLKLSQKKSIDFRVSTLPTLWGEKIVMRILDSDSAMLGIDILGYEAEQKQLYLDALAQPQGM
ILVTGPTGSGKTVSLYTGLNILNTEETNISTAEDPVEINLPGINQVQVNPRAGLTFPSALKAFLRQDPDVIMVGEIRDIE
TAEIAIKAAQTGHLVLSTLHTNSAPETLTRLLNMGVPAYNVASSVTLIIAQRLARRLCNHCKAAEQLPEQEALKQGFSAT
QLSQLKLFKPVGCDHCTGGYKGRLGIYEVMPITAQIAAKIMAGANSLDIAAQAQIEGFPNLRQSALKKAAAGFTSLAEVN
RVTNA

Nucleotide


Download         Length: 1698 bp        

>NTDB_id=332463 EK374_RS17890 WP_127025894.1 3958943..3960640(+) (pilB) [Rheinheimera mangrovi strain LHK132]
ATGGCATTAAACCCAAGCTCAATTTTACTAAAACGTTTGCACCAGGCGAATCTGGTCGACCCACAGATCACCGCTAAACT
GCTGCATGAAAAAAGTCATCTGTATCAAACCATTGCTGATCTGCTGATAGGTGAAAAACTGATCCAGTCAAAAGTCCTGG
CAGATGCAGCCGCCTCTTTTACCTTACACAGCTCCATGGATTTAGACTTTTTTGATATCAATGCCATTCCAGAGCAGCTA
CGTAATGAAAAGCTCATTCGCAAACACCATATTCTGCCGCTGAGTAAAAGGCACAAAACCTTATTTCTGGCGGTAGTCGA
TCCAACAGATATGACGTCTGTGGAAGATTTTGAATTTAATACCGGCCTGAACGCCGAAATGATAGTGGTCGAGTTTGATA
AGCTCCACAAAGCCATCGACAAATTATTTGAAACTACGGCCACCGACAGCTTTACCGACACCCAATGGGATTTAGCCAAA
ATGGGTTTGGCGGGCGGTGAAGAAGAAGACACTAAAACTGTGGGGTCGGACAGTGAAGATCAGCCTATTATTTCTTTTAT
TAACAAGATGCTGCACGACGCTATCCGTAAGGGGGCTTCGGATCTGCATTTTGAGCCTTACGAAAAAAGCTACCGTATCC
GTTTTCGGATTGATGGTATTTTGCATGAAGTCGCATCACCCCCAGTCGCTTTGTCTACCCGTTTGTCCGCCCGTTTAAAA
GTAATGTCGAGATTAGACATAGCTGAAAAGCGTGTACCGCAAGATGGACGCATTAAACTTAAACTGTCGCAAAAAAAATC
GATCGATTTTCGGGTCAGTACCTTGCCGACCTTATGGGGCGAAAAAATAGTGATGCGGATCCTCGACTCTGACAGCGCCA
TGTTAGGCATTGATATTCTGGGTTATGAGGCCGAACAAAAGCAGCTGTATCTGGACGCTTTAGCCCAACCGCAAGGCATG
ATTCTGGTGACAGGCCCGACAGGCTCAGGCAAAACTGTCTCTTTGTATACAGGGCTGAATATTCTCAATACCGAAGAAAC
CAATATATCCACAGCTGAAGATCCGGTTGAAATCAATCTGCCAGGTATTAATCAGGTGCAGGTCAATCCAAGAGCCGGCC
TGACCTTCCCTTCGGCTTTAAAAGCTTTTTTGCGTCAGGACCCTGATGTCATTATGGTGGGCGAAATCCGGGATATTGAA
ACCGCTGAAATCGCTATCAAAGCAGCGCAGACCGGCCATTTAGTCTTAAGCACTTTACACACCAACTCAGCTCCTGAAAC
CTTAACCCGCTTGCTGAACATGGGCGTGCCAGCCTACAACGTCGCAAGTTCTGTCACATTGATTATCGCTCAGCGGCTGG
CACGGCGCTTATGTAATCACTGCAAAGCGGCTGAACAGTTACCCGAACAGGAAGCGTTAAAACAAGGATTTTCTGCCACG
CAGCTCAGTCAACTCAAACTGTTTAAACCTGTGGGTTGTGATCATTGTACAGGGGGTTATAAAGGCCGTTTGGGTATTTA
TGAAGTGATGCCGATCACGGCGCAGATTGCAGCTAAGATTATGGCAGGAGCTAATTCGCTGGATATTGCCGCTCAGGCCC
AGATTGAAGGATTCCCAAACTTACGCCAGTCGGCTTTAAAAAAAGCAGCTGCTGGCTTCACCAGCCTTGCCGAAGTGAAC
CGGGTGACTAACGCCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(189-456)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(47-146)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

55.376

98.761

0.547
  pilB Acinetobacter baumannii D1279779

58.476

92.92

0.543
  pilB Legionella pneumophila strain ERS1305867

56.401

95.398

0.538
  pilB Vibrio cholerae strain A1552

55.865

89.027

0.497
  pilF Neisseria gonorrhoeae MS11

50

99.469

0.497
  pilB Vibrio parahaemolyticus RIMD 2210633

52.533

94.336

0.496
  pilB Vibrio campbellii strain DS40M4

52.166

93.982

0.49
  pilF Thermus thermophilus HB27

37.071

97.876

0.363

Multiple sequence alignment