Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   EKH72_RS03130 Genome accession   NZ_CP034565
Coordinates   587697..588920 (-) Length   407 a.a.
NCBI ID   WP_005479682.1    Uniprot ID   Q87LT6
Organism   Vibrio parahaemolyticus strain D3112     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 582697..593920
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EKH72_RS03090 rpsP 582740..582988 (+) 249 WP_005379962.1 30S ribosomal protein S16 -
  EKH72_RS03095 rimM 583017..583565 (+) 549 WP_005462552.1 ribosome maturation factor RimM -
  EKH72_RS03100 trmD 583593..584336 (+) 744 WP_005462562.1 tRNA (guanosine(37)-N1)-methyltransferase TrmD -
  EKH72_RS03105 rplS 584378..584731 (+) 354 WP_005462554.1 50S ribosomal protein L19 -
  EKH72_RS03110 yacG 585117..585311 (-) 195 WP_005462546.1 DNA gyrase inhibitor YacG -
  EKH72_RS03115 zapD 585380..586120 (-) 741 WP_015297254.1 cell division protein ZapD -
  EKH72_RS03120 coaE 586148..586762 (-) 615 WP_129829844.1 dephospho-CoA kinase -
  EKH72_RS03125 pilD 586763..587632 (-) 870 WP_129829845.1 A24 family peptidase Machinery gene
  EKH72_RS03130 pilC 587697..588920 (-) 1224 WP_005479682.1 type II secretion system F family protein Machinery gene
  EKH72_RS03135 pilB 588945..590630 (-) 1686 WP_129829846.1 type IV-A pilus assembly ATPase PilB Machinery gene
  EKH72_RS03140 pilA 590631..591044 (-) 414 WP_029831396.1 pilin Machinery gene
  EKH72_RS03145 nadC 591307..592194 (-) 888 WP_015297249.1 carboxylating nicotinate-nucleotide diphosphorylase -
  EKH72_RS03150 ampD 592287..592838 (+) 552 WP_129830503.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -

Sequence

Protein


Download         Length: 407 a.a.        Molecular weight: 45220.39 Da        Isoelectric Point: 10.4368

>NTDB_id=331929 EKH72_RS03130 WP_005479682.1 587697..588920(-) (pilC) [Vibrio parahaemolyticus strain D3112]
MKTTTPQLKNFRWKGVNSSGKKTSGQTLAMSEIEVRERLDAQHIKIKKLKKSSISFLTKLSHRVKGKDITVFTRQISTML
VTGVPLVQALKLVSDNHKKAEMKSILMSVTRAVEAGTPMSKAMRTASNHFDPLYTDLIATGEQSGNLAEVFERLATYREK
NEQLRAKVIKALIYPAMVILVALGVSFIMLTKVIPEFEKMFVGFGAELPWFTRQVLDLSAWTQNWSPFIALGSISVFISA
RVLSKRSDSFRLMLNRSVLKFPVLGAVLSKAAIAKFSRTLATSFTAGIPILTSLKTTSKTSGNMHYQLAIEEVYRDTAAG
MPMYVAMRNCNVFPELVLQMVMIGEESGRLDDMLNKVATIYEFEVDNTVDNLSKILEPLIIVFLGIVVGGLVTAMYLPIF
NLMSVLG

Nucleotide


Download         Length: 1224 bp        

>NTDB_id=331929 EKH72_RS03130 WP_005479682.1 587697..588920(-) (pilC) [Vibrio parahaemolyticus strain D3112]
ATGAAAACTACGACACCACAATTAAAGAACTTCCGTTGGAAAGGCGTCAACAGCTCAGGGAAAAAGACCTCTGGTCAAAC
TCTGGCAATGAGCGAAATCGAAGTACGAGAGCGCTTAGATGCCCAGCACATCAAAATCAAAAAGCTAAAGAAAAGCAGCA
TCTCTTTTTTGACCAAACTTAGCCATCGCGTAAAAGGAAAAGACATCACGGTTTTCACTCGTCAAATCTCAACGATGCTA
GTAACTGGTGTGCCGCTAGTACAAGCATTGAAATTGGTCTCTGATAACCACAAAAAGGCGGAAATGAAATCCATTTTGAT
GAGTGTAACTCGGGCTGTTGAAGCGGGTACGCCGATGTCCAAAGCGATGCGTACCGCCAGTAACCATTTTGACCCGCTGT
ATACCGACTTGATTGCGACAGGAGAACAATCGGGCAACTTAGCCGAAGTGTTCGAACGCTTAGCCACCTACAGGGAAAAA
AATGAGCAGCTTAGAGCCAAAGTGATCAAGGCTCTAATATATCCAGCCATGGTTATTCTAGTTGCACTGGGAGTATCGTT
TATCATGCTAACCAAGGTCATTCCCGAGTTTGAAAAAATGTTTGTTGGCTTTGGTGCTGAATTGCCATGGTTTACAAGGC
AAGTCTTAGATCTTTCCGCTTGGACACAAAACTGGAGCCCATTTATCGCACTTGGCTCCATCAGTGTATTTATCTCCGCG
AGAGTACTCTCTAAGCGTTCAGATTCTTTTCGTTTAATGCTTAACCGATCTGTACTTAAGTTTCCAGTCCTTGGGGCGGT
TTTATCGAAAGCCGCTATCGCAAAATTTAGTCGAACACTCGCTACGAGCTTTACGGCGGGCATTCCGATTCTAACCAGTT
TAAAAACCACCTCGAAAACGTCCGGAAATATGCATTATCAATTGGCTATCGAAGAGGTTTATCGCGATACCGCAGCAGGA
ATGCCGATGTATGTTGCAATGCGCAATTGCAACGTGTTCCCTGAGTTGGTGCTGCAAATGGTTATGATCGGCGAAGAATC
CGGCCGACTTGACGATATGCTCAATAAAGTCGCCACAATTTACGAGTTTGAAGTGGACAACACCGTCGATAACCTCAGTA
AAATCTTAGAACCATTAATTATCGTTTTTTTAGGTATCGTTGTCGGTGGCTTGGTCACAGCAATGTACTTGCCAATCTTT
AACTTAATGAGTGTATTGGGCTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(276-398)

Type II secretion system (T2SS), protein F (T2SSF)

(72-195)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q87LT6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Vibrio campbellii strain DS40M4

85.995

100

0.86
  pilC Vibrio cholerae strain A1552

74.321

99.509

0.74
  pilC Acinetobacter baylyi ADP1

42.75

98.28

0.42
  pilC Acinetobacter baumannii D1279779

41.439

99.017

0.41
  pilG Neisseria gonorrhoeae MS11

40.494

99.509

0.403
  pilG Neisseria meningitidis 44/76-A

40.247

99.509

0.4
  pilC Pseudomonas stutzeri DSM 10701

40.909

97.297

0.398
  pilC Legionella pneumophila strain ERS1305867

39.803

100

0.398

Multiple sequence alignment